DOI QR코드

DOI QR Code

Role of oyster mushroom as an alternative fermentation source in the growth of white-spotted flower chafer(Protaetia brevitarsis seulensis)

사료 발효원으로 느타리버섯 종균이 흰점박이꽃무지 생육에 미치는 영향

  • Mi-Hye Kim (Osangkinsect Co., Ltd.) ;
  • Jang-Woo Park (Osangkinsect Co., Ltd.) ;
  • Mi-Jung Kim (Osangkinsect Co., Ltd.) ;
  • Jung-Joon Park (Department of Plant Medicine, Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2021.11.03
  • Accepted : 2021.11.23
  • Published : 2021.12.31

Abstract

Effective microorganisms (EM), which are sources of fermentation byproducts in herbal medicine, were compared with oyster mushrooms(OM) to identify alternative larval feeds for white-spotted flower chafer (Protaetia brevitarsis seulensis). The nutritional composition of each fermented feed was analyzed. The content of crude protein, crude fat, and fiber was higher in the OM fermented feed, except for crude ash. No difference in nutritional content of larvae based on fermented feed was observed. A comparative weekly analysis of the live weights of larvae was based on the fermented feed used. The average weight was significantly higher in the feeds using EM and OM from third week of observation. The larval survival rate in the presence of fermented feed was 96.7% compared with 9.8% with non-fermented feed. The results suggest that fermented food source is essential for the growth of white-spotted flower chafer, and OM was a stable alternative to EM as a fermentation source for the survival of white-spotted flower chafer.

흰점박이꽃무지(Protaetia brevitarsis seulensis) 유충의 대체사료인 한약재 부산물 발효원인 유용미생물(Effective microorganism, EM)과 느타리버섯 종균(Oyster mushroom fungi, OM)을 비교하였다. 각 발효된 사료의 영양성분을 비교한 결과, 조회분을 제외한 조단백, 조지방, 조섬유 함량이 OM 발효사료에서 높게 나타났다. 각 발효된 사료로 사육된 흰점박이꽃무지 유충의 영양성분을 비교한 결과 차이가 관찰되지 않았다. 각 발효사료별 흰점박이꽃무지 유충의 생체중을 주별 비교 분석한 결과, 3주차 관찰시기부터 EM과 OM을 이용한 사료에서 흰점박이꽃무지 유충 평균중량이 유의하게 높았다. 유충 사육 시 생존율은 발효사료의 경우 동일하게 96.7%이나, 비발효사료의 경우 9.8%로 매우 낮았다. 본 실험결과, 흰점박이꽃무지의 생육에 먹이 원의 발효는 꼭 필요했으며, OM은 EM을 대체할 수 있는 흰점박이꽃무지 대체사료의 발효원으로 더 안정적이었다.

Keywords

Acknowledgement

본 연구는 의령군 항노화 기술개발·업화 지원사업(세부과제명: 흰점박이꽃무지를 활용한 고령친화식품원료 기술개발)의 지원에 의해 이루어졌습니다.

References

  1. Anon. 1995. Effective Microorganisms. EM Application Manual for the Asia-Pacific Natural Agricultural Network (APNAN) Countries. Future Tech Today Inc. Eugene, OR. http://www.futuretechtoday.net/em/app.htm
  2. AOAC. 2000. Official Methods of Analysis of AOAC International. 17th ed. Association of Analytical Communities. Gaithersburg, MD, pp. 1-26.
  3. Bukkens SGF. 1997. The nutritional value of edible insects. Ecol. Food Nutr. 36:287-319. https://doi.org/10.1080/03670244.1997.9991521
  4. Cho DH, YM Cho and JI Lee. 2003. Fruitbody formation of Cordyceps militaris in Allomyrina dichotoma Linnaeus. Korean J. Plant Res. 16:1-7.
  5. Cho PS. 1969. Illustrated Encyclopedia of Fauna & Flora of Korea. Vol. 10. Insecta (2). Samhwa Publ. Seoul. pp. 686-693.
  6. Chung MY, EY Gwon, JS Hwang, TW Goo and EY Yun. 2013. Analysis of general composition and harmful material of Protaetia brevitarsis. J. Life Sci. 23:664-668. https://doi.org/10.5352/JLS.2013.23.5.664
  7. Condor AF, P Gonzalez and C Lakre. 2007. Effective microorganisms: Myth or reality? Rev. Peru. Biol. 14:315-319. https://doi.org/10.15381/rpb.v14i2.1837
  8. El-Enshasy HA. 2007. Filamentous fungal cultures - Process characteristics, products, and applications. pp. 225-261. In: Bioprocessing for Value-Added Products from Renewable Resources: New Technologies and Applications(Yang ST ed.). Elsevier. Netherlands.
  9. FAO. 2013. The State of Food Insecurity in the World, 2013: The Multiple Dimensions of Food Security. Food and Agriculture Organization of the United States. Washington, D.C.
  10. Ghorai S, SP Banik, D Verma, S Chowdhury, S Mukherjee and S Khowala. 2011. Fungal biotechnology in food and feed processing. pp. 603-615. In: Comprehensive Biotechnology(2nd Edition)(Moo-Young M ed.). Elsevier. Netherlands.
  11. Higa T and JF Parr. 1994 Beneficial and Effective Microorganisms for a Sustainable Agriculture and Environment. Japan International Nature Farming Research Center. Nagano, Japan. p. 7. Retrieved 14 August 2016.
  12. Hwang SY, YB Kim, SH Lee and CY Yun. 2005. Preventive effect of a chafer, Protaetia brevitarsis extract on carbon tetrachloride-induced liver injuries in rats. Korean J. Orient. Physiol. Pathol. 19:1337-1343.
  13. Jun JH, BS Ko, JH Kim, SP Nam, YR Um, SM Hong, HS Hwang and SM Park. 2009. The comparison of growth and quality characteristics during the storage of Pleurotus ostreatus cultivated in the remnants of medicinal herb extracts. J. Korean Soc. Food Sci. Nutr. 38:211-216.
  14. Kan F, J Sun and L Tian. 2009. Isolation and proliferation effect of polysaccharide extracted from grub on the immunocytes of mice in vitro. Nanjing Nongye Daxue Xuebao 32:161-164.
  15. Kang IJ, HK Kim, CK Chung, SJ Kim and SH Oh. 2000. Effects of Protaetia orientalis (Gory et Perchlon) larva on the lipid metabolism in ethanol administered rats. J. Korean Soc. Food Sci. Nutr. 29:479-484.
  16. Kang MG, CG Kang, HK Lee, EK Kim, JS Kim, OS Kwon, HK Lee, HJ Kang, CH Kim and HS Jang. 2012. Effects of fermented aloe vera mixed diet on larval growth of Protaetia brevitarsis seulensis (Kolbe) (coleopteran: Cetoniidae) and protective effects of its extract against CCl4 -induced hepatotoxicity in Sprague-Dawley rats. Entomol. Res. 42:111-121. https://doi.org/10.1111/j.1748-5967.2012.00444.x
  17. Kang SJ, CW Park, SC Han, YK Yi and YG Kim. 2005. A grub (Protaetia brevitarsis seulensis) rearing technique using cellulose-digesting bacteria and natural recycling of rearing byproduct to an organic fertilizer. Korean J. Appl. Entomol. 44:189-197.
  18. Kim CH, JS Lee, MS Go and KT Park. 2002. Ecological characteristics of Protaetia orientalis submarmorea (Burmeister) (Coloptera: Cetoniidae). Korean J. Appl. Entomol. 41:43-47.
  19. Kim HG and KH Kang. 2005. Bionomical characteristic of Protaetia brevitarsis. Korean J. Appl. Entomol. 44:139-144.
  20. Kim HG and KH Kang. 2006. Imago's flight and larval activities of Protaetia brevitarsis (Coleoptera: Scarabaedia) and Allomyrina dichotoma (Coleoptera: Dynastinae). Korean J. Appl. Entomol. 45:139-143.
  21. Kim MH, JW Park, MJ Kim and JJ Park. 2019. Effect of herbal medicine by-products on the larval growth of white-spotted flower chafer(Protaetia brevitarsis seulensis). Korean J. Environ. Biol. 37:60-67. https://doi.org/10.11626/KJEB.2019.37.1.060
  22. Kim YS, CG Park, T Kim and JW Choi. 2018. A study on the legal status of insect industry. Korean J. Appl. Entomol. 57:401-408.
  23. Kohlerab R, L Kariukia, C Lamberta and HK Biesalskia. 2019. Protein, amino acid and mineral composition of some edible insects from Thailand. J. Asia-Pac. Entomol. 22:372-378. https://doi.org/10.1016/j.aspen.2019.02.002
  24. Lee HC, SG Hwang, YK Kang, HO Sohn, JY Moon, HB Lim, BH Jeon and DW Lee. 2001. Influence of Protaetia brevitarsis extract on liver damage induced by carbon tetra chloride and ethanol in rat. Korean J. Life Sci. 11:405-414.
  25. Lee JH. 2011. Study on the application of RFID system for the improvement of circulation structure of medicinal herbs. Graduate school of Inje University. Gimhae, Korea. pp. 24-34.
  26. Lee SB, JW Kim, SM Bae, YH Hwang, BJ Lee, KP Hong and CG Park. 2018. Evaluation of spent mushroom substrates as food for white-spotted flower chafer, Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae). Korean J. Appl. Entomol. 57:97-104.
  27. Mohan B. 2008. Evaluation of organic growth promoters on yield of dryland vegetable crops in India. J. Org. Syst. 3:23-36.
  28. NIFDS and NIAS. 2021. Accredited as Food Raw Material for Locusta migratoria. National Institute of Food and Drug Safety Evaluation and National Institute of Agricultural Science. Korea. Press Release at 13 September, 2021. p. 8.
  29. Park HY, SS Park, HW Oh and JI Kim. 1994. General characteristics of the white-spotted flower chafer, Protaetia breviratsis reared in the laboratory. Korean J. Entomol. 24:1-5.
  30. Park JH, SY Kim, M Kang, M Yoon, YI Lee and E Park. 2012. Antioxidant activity and safety evaluation of juice containg Protaetia brevitarsis. J. Korean Soc. Food Sci. Nutr. 41:41-48. https://doi.org/10.3746/jkfn.2012.41.1.041
  31. RDA. 2016. Insect Industry. Human Resource Development. Rural Development Administration. Jeonju, Korea.
  32. RDA. 2017. The Standards and Qualification of Industrial Insects. National Institute of Agricultural Sciences, Rural Development Administration. Jeonju, Korea.
  33. Roupas P, J Keogh, M Noakes, C Margetts and P Taylor. 2012. The role of edible mushrooms in health: Evaluation of the evidence. J. Funct. Food. 4:687-709. https://doi.org/10.1016/j.jff.2012.05.003
  34. SAS Institute. 2010. SAS/STAT(R) 9.22 User's Guide. Cary, NC.
  35. Stanley-Samuelson DW, RA Jurenka, C Cripps, GJ Blomquist and M de Renobales. 1988. Fatty acids in insects: Composition, metabolism, and biological significance. Arch. Insect Biochem. Physiol. 9:1-33.
  36. Stierle AA and DB Stierle. 2000. Bioactive compounds from four Endophytic Penicillium sp. of a Northwest Pacific Yew Tree. pp. 933-977. In: Studies in Natural Products Chemistry-Bioactive Natural Products (V. 24, Part E) (Atta-ur-Rahman ed.). Elsevier. Netherlands.
  37. Suo Z, M Bai, S Li, HD Yang, T Li and D Ma. 2015. A geometric morphometric analysis of the morphological variations among Chinese populations of Protaetia brevitarsis (Coleoptera: Scarabaeidae) with an inference of the invading source of its Xinjiang populations. Acta Entomol. Sin. 58:408-418.
  38. Van Itterbeeck and van Huis. 2012. Environmental manipulation for edible insect procurement, a historical perspective. J. Ethnobiol. Ethnomed. 8:3-10. https://doi.org/10.1186/1746-4269-8-3
  39. Wang L and ST Yang. 2007. Solid state fermentation and its applications. pp. 465-489. In: Bioprocessing for Value-Added Products from Renewable Resources: New Technologies and Applications (Yang ST ed.). Elsevier. Netherlands.
  40. Yoon CH, HS Song, BJ Lee, D Son, SH Jeon and YS Cho. 2016. Effects of feeds on larval development of white-spotted flower chafer, Protaetia brevitarsis seulensis. J. Korean Soc. Int. Agric. 28:541-546. https://doi.org/10.12719/KSIA.2016.28.4.541
  41. Zhang ZL. 1984. Economic Insect Fauna of China. Fascicle. 28. Coleoptera: Larva of Scarabaeidae. Science Press. Beijing.