대한의생명과학회지 (Biomedical Science Letters)

  • 제27권2호
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  • Pages.51-58
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  • 2021
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
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Korean Red Ginseng and Portulaca oleracea Extracts Attenuate Lipopolysaccharide-induced Inflammation via Downregulation of Nuclear Factor Kappa-B and the Mitogen-activated Protein Kinase Signaling Pathway in Macrophage Cell Line RAW 264.7

  • Ullah, HM Arif (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Tae-Hwan (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Saba, Evelyn (Department of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah-Arid Agriculture University) ;
  • Kim, Sung Dae (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee, Man Hee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University)
  • 투고 : 2021.04.30
  • 심사 : 2021.06.04
  • 발행 : 2021.06.30
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초록

Korean red ginseng (Panax ginseng Meyer) is a well-known traditional medicine, with numerous biological functions in the body. Portulaca oleracea (P. ole) belongs to the Portulacaceae family and has bioactive potential as a traditional medicine. This study aimed to determine the anti-inflammatory effects of Korean red ginseng extract (RGE) and P. ole extract on lipopolysaccharide (LPS)-treated RAW 264.7 cells. The combination of RGE (50 ㎍/mL) and P. ole (6.25 ㎍/mL) extracts significantly suppressed LPS-induced nitric oxide synthesis. The expression of proinflammatory mediators, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and proinflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α, were markedly decreased by the combined treatment with RGE (50 ㎍/mL) and P. ole (6.25 ㎍/mL). Moreover, iNOS and COX-2 protein expression levels were also significantly reduced in the combined treatment compared to the LPS-stimulated group. In addition, the nuclear translocation of phosphorylated nuclear factor kappa-B was suppressed by the treatment with RGE and P. ole. Moreover, the mitogen-activated protein kinase pathway was also partially inhibited by the combination treatment with RGE and P. ole. Our results demonstrate that the treatment mixture with RGE and P. ole could be used as functional food and therapeutic herbal medicine in various inflammatory diseases.

키워드

과제정보

This study was funded by the National Research Foundation of Korea (2018R1D1A1A09083797). The authors would like to thank Professor Man Hee Rhee for his supervision and support, H M Arif Ullah for the manuscript writing and final review and editing and Yuan Yee Lee for technical support.

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