Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Anti-Inflammatory Effect of Pulsed Electromagnetic Field on DNCB-Induced Atopic Dermatitis Using Principal Component Analysis

주성분 분석을 이용한 펄스형 전자기장 자극을 통해 DNCB로 유발된 아토피성 피부염의 개선 효과 분석

  • Lee, Jiyoung (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, Jun-Yong (Department of Biomedical Engineering, Yonsei University) ;
  • Lee, Yerin (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, Ko Eun (Department of Dermatology, Korea University Ansan Hospital, Korea University) ;
  • Lee, Yongheum (Department of Biomedical Engineering, Yonsei University) ;
  • Yang, Sejung (Department of Biomedical Engineering, Yonsei University)
  • 이지영 (연세대학교 보건과학대학 의공학부) ;
  • 김준영 (연세대학교 보건과학대학 의공학부) ;
  • 이예린 (연세대학교 보건과학대학 의공학부) ;
  • 김고은 (고려대학교 안산병원 피부) ;
  • 이용흠 (연세대학교 보건과학대학 의공학부) ;
  • 양세정 (연세대학교 보건과학대학 의공학부)
  • Received : 2021.04.01
  • Accepted : 2021.06.21
  • Published : 2021.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Atopic dermatitis (AD), a chronic inflammatory skin disease, is characterized by itchy and age-dependent lesions. Previous studies have shown that pulsed electromagnetic field (PEMF) significantly improved chronic ulcers and ununited fractures, providing an evidence for the application of PEMF in resolving inflammation caused by AD. This study investigated the anti-inflammatory effect of PEMF on DNCB-induced AD in animal models. Five male hairless mice (6 weeks old) per group were assigned to a normal group, a sham group, and two PEMF groups (15Hz, 75Hz). Mice were treated with 2,4-Dinitrochlorobenzene (DNCB) to induce uniform AD among all groups excluding a normal group. To examine the inflammatory progress and the improvement of AD after the PEMF stimulation, images are taken with various cameras for non-invasive evaluation and the results are expressed using principal component analysis (PCA) for visualization. The results of this study demonstrated that PEMF effectively improved skin lesions without the use of drugs.

Keywords

Acknowledgement

본 연구는 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행하였음(No. 2019R1F1A1058971).

References

  1. Leung DY. Atopic dermatitis: new insights and opportunities for therapeutic intervention. Journal of Allergy and Clinical Immunology. 2000;105(5):860-876. https://doi.org/10.1067/mai.2000.106484
  2. Zeiger RS, Heller S. The development and prediction of atopy in high-risk children: follow-up at age seven years in a prospective randomized study of combined maternal and infant food allergen avoidance. Journal of Allergy and Clinical Immunology. 1995;95(6):1179-1190. https://doi.org/10.1016/S0091-6749(95)70074-9
  3. Gupta RS, Springston EE, Warrier MR, Smith B, Kumar R, Pongracic J, Holl JL. The prevalence, severity, and distribution of childhood food allergy in the United States. Pediatrics. 2011;128(1):e9-e17. https://doi.org/10.1542/peds.2011-0204
  4. Floistrup H, Swartz J, Bergstrom A, Alm JS, Scheynius A, Van Hage M, Waser M, Braun-Fahrlander C, Schram-Bijkerk D, Huber M. Allergic disease and sensitization in Steiner school children. Journal of Allergy and Clinical Immunology. 2006; 117(1):59-66. https://doi.org/10.1016/j.jaci.2005.09.039
  5. Van Cauwenberge P, Bachert C, Passalacqua G, Bousquet J, Canonica G, Durham S, Fokkens W, Howarth P, Lund V, Malling HJ. Consensus statement* on the treatment of allergic rhinitis. Allergy. 2000;55(2):116-134. https://doi.org/10.1034/j.1398-9995.2000.00526.x
  6. Milgrom H, Fick Jr RB, Su JQ, Reimann JD, Bush RK, Watrous ML, Metzger WJ. Treatment of allergic asthma with monoclonal anti-IgE antibody. New England Journal of Medicine. 1999;341(26):1966-1973. https://doi.org/10.1056/NEJM199912233412603
  7. Watson WT, Becker AB, Simons FER. Treatment of allergic rhinitis with intranasal corticosteroids in patients with mild asthma: effect on lower airway responsiveness. Journal of Allergy and Clinical Immunology. 1993;91(1):97-101. https://doi.org/10.1016/0091-6749(93)90301-U
  8. Callaghan MJ, Chang EI, Seiser N, Aarabi S, Ghali S, Kinnucan ER, Simon BJ, Gurtner GC. Pulsed electromagnetic fields accelerate normal and diabetic wound healing by increasing endogenous FGF-2 release. Plastic and reconstructive surgery. 2008;121(1):130-141. https://doi.org/10.1097/01.prs.0000293761.27219.84
  9. Choi HM, Cheing AK, Ng GY, Cheing GL. Effects of pulsed electromagnetic field (PEMF) on the tensile biomechanical properties of diabetic wounds at different phases of healing. PloS one. 2018;13(1):e0191074. https://doi.org/10.1371/journal.pone.0191074
  10. Lee Y, Choi HK, N'deh KPU, Choi Y-J, Fan M, Kim E-K, Chung K-H, An JH. Inhibitory effect of Centella asiatica extract on DNCB-Induced atopic dermatitis in HaCaT cells and BALB/c mice. Nutrients. 2020;12(2):411. https://doi.org/10.3390/nu12020411
  11. Bassett CaL, Mitchell SN, Gaston SR. Pulsing electro-magnetic field treatment in ununited fractures and failed arthrodeses. Jama. 1982;247(5):623-628. https://doi.org/10.1001/jama.1982.03320300027017
  12. Gossling HR, Bernstein RA, Abbott J. Treatment of ununited tibial fractures: a comparison of surgery and pulsed electromagnetic fields (PEMF). Orthopedics. 1992;15(6):711-719. https://doi.org/10.3928/0147-7447-19920601-08
  13. Bassett CaL. Fundamental and practical aspects of therapeutic uses of pulsed electromagnetic fields (PEMFs). Crit Rev Biomed Eng. 1989;17(5):451-529.
  14. Uehara M, Sawai T. A longitudinal study of contact sensitivity in patients with atopic dermatitis. Archives of dermatology. 1989;125(3):366-368. https://doi.org/10.1001/archderm.1989.01670150056006
  15. Matsumoto K, Mizukoshi K, Oyobikawa M, Ohshima H, Tagami H. Establishment of an atopic dermatitis-like skin model in a hairless mouse by repeated elicitation of contact hypersensitivity that enables to conduct functional analyses of the stratum corneum with various non-invasive biophysical instruments. Skin Research and Technology. 2004;10(2):122-129. https://doi.org/10.1111/j.1600-0846.2004.00062.x
  16. Wold S, Esbensen K, Geladi P. Principal component analysis. Chemometrics and intelligent laboratory systems. 1987;2(1-3): 37-52. https://doi.org/10.1016/0169-7439(87)80084-9