Journal of Ginseng Research

  • 제45권3호
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  • Pages.456-463
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  • 2021
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Korean Red Ginseng attenuates ultraviolet-mediated inflammasome activation in keratinocytes

  • Ahn, Huijeong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Han, Byung-Cheol (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Hong, Eui-Ju (College of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University) ;
  • An, Beum-Soo (Department of Biomaterial Science, College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Eunsong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Lee, Seung-Ho (Korea Ginseng Research Institute, Korea Ginseng Corporation) ;
  • Lee, Geun-Shik (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2020.10.13
  • 심사 : 2021.02.09
  • 발행 : 2021.05.01
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초록

Background: Keratinocytes form a physical barrier and act as an innate immune cell in skin. Keratinocytes secrete pro-inflammatory cytokines, such as interleukin (IL)-1β, resulting from inflammasome activation when exposed to ultraviolet (UV) irradiation. Korean Red Ginseng extracts (RGE) have been well-studied as modulators of inflammasome activation in immune cells, such as macrophages. In the study, we elucidated the role of RGE on the UV-mediated inflammasome activation in keratinocytes compared with that in macrophages. Methods: Human skin keratinocyte cells (HaCaT), human epidermal keratinocytes (HEK), human monocyte-like cells (THP-1), and mouse macrophages were treated with RGE or a saponin fraction (SF) or non-saponin fraction (NS) of RGE before and after UV irradiation. The secretion levels of IL-1β, as an indicator of inflammasome activation, were analyzed. Results: The treatment of RGE or SF in macrophages after UV irradiation inhibited IL-1β secretion, but similar treatment in HaCaT cells did not. However, the treatment of RGE or SF in HaCaT cells in the presence of poly I:C, a toll-like receptor (TLR) 3 ligand, before UV exposure elicited the inhibition of the IL-1β secretion. The inhibition was caused by the disruption by RGE or SF of the TLR mediating up-regulation of the pro-IL-1β and NLRP3 genes during the priming step. Conclusion: RGE and its saponins inhibit IL-1β secretion in response to UV exposure in both keratinocytes and macrophages. In particular, RGE treatment interrupted only the priming step in keratinocytes, although it did attenuate both the priming and activation steps in macrophages.

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과제정보

This research was supported by a grant (2019) from the Korean Society of Ginseng funded by the Korean Ginseng Corporation and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2018R1A2B2004097 and NRF-2018R1D1A1B07048337).

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