Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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The effects of sesame oil and different doses of estradiol on testicular structure, sperm parameters, and chromatin integrity in old mice

  • Mohammadzadeh, Masoomeh (Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences) ;
  • Pourentezari, Majid (Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences) ;
  • Zare-Zardini, Hadi (Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences) ;
  • Nabi, Ali (Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences) ;
  • Esmailabad, Saeed Ghasemi (Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences) ;
  • Khodadadian, Ali (Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences) ;
  • Talebi, Ali Reza (Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences)
  • Received : 2020.01.14
  • Accepted : 2020.04.25
  • Published : 2021.03.31
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Abstract

Objective: Studies of the effects of estrogens on the male reproductive system have emphasized the role of these hormones in male fertility. Sesame oil has many phytoestrogenic compounds and may improve male fertility. This study investigated the effects of sesame oil and different concentrations of estrogen on sperm parameters and DNA integrity in male mice. Methods: Twenty old NMRI (The Naval Medical Research Institute) male mice (40 weeks; weight, 30-35 g) were treated with sesame oil or different concentrations of estrogen (estradiol, 1 and 10 μL/kg/day) or received no treatment (controls). After 35 days, sperm parameters and DNA integrity were assessed and analyzed. Results: Sperm count, progressive motility, and morphology were decreased in the group that received 10 μL/kg of estradiol. A remarkably lower percentage of DNA fragmentation and protamine deficiency were detected in the group that received 1 μL/kg of estradiol. In the groups that received sesame oil and 1 μL/kg of estradiol, the numbers of spermatogonia and Leydig cells were higher than in controls. The combination of sesame oil and 1 μL/kg of estradiol led to improved sperm parameters and chromatin and testicular structure. Conclusion: Based on this study, consumption of sesame oil and a low concentration of estradiol may improve testicular function in older mice.

Keywords

Acknowledgement

The authors would like to thank Yazd Reproductive Science Institute for financial support of the current study.

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