The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Anti-inflammatory Effects of Ojeok-san in LPS-induced Inflammatory Rat Model

LPS유도 염증 동물모델에서 오적산의 항염증 효과

  • Kim, Joo-Hee (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Sangji University) ;
  • Lee, Dong Hyuk (College of Korean Medicine, Sangji University) ;
  • Kim, Ji Hye (National Development Institute of Korean Medicine) ;
  • Jung, Sung Eun (National Development Institute of Korean Medicine) ;
  • Ham, Seong Ho (National Development Institute of Korean Medicine) ;
  • Yang, Woong Mo (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University) ;
  • Kwon, Bo-in (Department of Pathology, College of Korean Medicine, Sangji University)
  • 김주희 (상지대학교 한의과대학 침구의학교실) ;
  • 이동혁 (상지대학교 한의과대학 한의기초과학교실) ;
  • 김지혜 (한국한의약진흥원) ;
  • 정성은 (한국한의약진흥원) ;
  • 함성호 (한국한의약진흥원) ;
  • 양웅모 (경희대학교 한의과대학 융합한의학교실) ;
  • 권보인 (상지대학교 한의과대학 병리학교실)
  • Received : 2021.01.29
  • Accepted : 2021.04.13
  • Published : 2021.06.01
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Abstract

Objectives: The aim of this study is to investigate the anti-inflammatory effects of Ojeok-san and compare the therapeutic effects according to its formation. Methods: We evaluated the anti-inflammatory effects of Ojeok-san using lipopolysaccharide (LPS) induced inflammatory animal model. Male SD rats were administered intra-orally with two different formulation types of Ojeok-san according to prescribed dosage. One hour later, to induce inflammatory responses, subsequent intra-peritoneal injection of LPS was conducted. After 5 hours later, serum TNF-α, IL-1β, IL-6 and PGE2 levels were measured by ELISA to assess the alteration of pro-inflammatory markers. Results: In our experiment, regardless of its formation, administration of Ojeok-san decreased TNF-𝛼, IL-1𝛽, IL-6 and PGE2 level in serum. Furthermore, LPS-induced toxicity of liver and kidney was not detected by Ojeok-san administration. Conclusions: The anti-inflammatory effect of Ojeok-san was shown in LPS-induced inflammatory model by decreasing pro-inflammatory markers, and there would be no significant difference in therapeutic effect between two formulation types of Ojeok-san.

Keywords

Acknowledgement

This work was supported by a grant from Pharmacological Standardization Project of Traditional Korean Medicine by Ministry of Health and welfare (MOHW) and JeollaNamdo, Republic of Korea.

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