BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Adipogenic function of tetranectin mediated by enhancing mitotic clonal expansion via ERK signaling

  • Go, Seulgi (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University) ;
  • Park, Jihyun (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University) ;
  • Rahman, Safikur (Department of Botany, Munshi Singh College, BR Ambedkar Bihar University) ;
  • Jin, Juno (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University) ;
  • Choi, Inho (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University) ;
  • Kim, Jihoe (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University)
  • 투고 : 2021.02.16
  • 심사 : 2021.03.06
  • 발행 : 2021.07.31
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초록

Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling.

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과제정보

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1A6A1A03044512) and Yeungnam University research grants in 2020.

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