The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of ethanol extract from Achyranthis Radix on hair growth

우슬의 에탄올 추출물이 모발 성장에 미치는 영향

  • Lee, Mi-Ja (Department of Public Health Graduate School, Daegu Haany University) ;
  • Choi, Moon-Yeol (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Yoo Jin (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Yoo, Wang Keun (Department of Health Science, Daegu Haany University)
  • 이미자 (대구한의대학교 일반대학원 보건학과) ;
  • 최문열 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김유진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 유왕근 (대구한의대학교 보건학부)
  • Received : 2021.06.14
  • Accepted : 2021.07.25
  • Published : 2021.07.30
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Abstract

Objective : As more and more people are interested in appearance in modern society, the increasing number of hair loss population can have an important impact on psychological and social problems such as depression and inappropriate interpersonal symptoms. Therefore, much research is being done on treatments for alopecia using herbal extracts with relatively few side effects. This study was investigated about the effect of Achyranthis Radix (AR) extract with ethanol solvent on hair growth. Methods : We determined the promoting efficacy of AR-ethanol extract compared with minoxidil (MNXD) on the growth of human hair dermal papilla cells (HDPCs). Cell viability was measured by MTT assay and cell proliferation was confirmed by cell cycle analysis from flow cytometry in HDPCs. Also, we monitored the safe concentration range through MTT assay. And protein expression of hair growth-related genes (insulin-like growth factor 1 (IGF-1), Wnt3a, Protein kinase B (Akt), Extracellular signal-regulated kinase (Erk)) was monitored by western blot. Results : On cell cycle analysis, the G2/M phase was higher than that of the DW group in AR ethanol extract group at 0.05 and 0.1 mg/㎖. All protein expression levels of HDPCs were increased in AR ethanol extract groups and the MNXD group, compared to the DW group, respectively. Conclusion : As mentioned above, AR extract increased cell proliferation and the protein expression of IGF-1, Wnt3a, Akt, Erk in HDPCs. These results suggest that AR ethanol extract has promoted hair growth and it might be potential hair growth supplement.

Keywords

References

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