Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Analysis of Life Cycle on Spodoptera exigua by Various Temperatures and Larval Susceptibility against Two Pesticides in Quarantine Pest Research Facility

검역해충실험동에서 파밤나방의 온도별 생활사 및 2종 살충제에 대한 유충 감수성 조사

  • Lee, Jieun (Plant Quarantine Technique Center, Animal and Plant Quarantine Agency) ;
  • Park, Youngjin (Plant Quarantine Technique Center, Animal and Plant Quarantine Agency)
  • 이지은 (농림축산검역본부 식물검역기술개발센터) ;
  • 박영진 (농림축산검역본부 식물검역기술개발센터)
  • Received : 2021.01.05
  • Accepted : 2021.02.18
  • Published : 2021.06.01
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Abstract

A Quarantine Pest Research Facility (QPRF) had been build in Animal and Plant Quarantine Agency and designed to ensure alien pests are safety contained inside the laboratory. Functions of QPRF including insect rearing, research, and physical containment facility were verified to check suitability as a research laboratory using the beet armyworm, Spodoptera exigua. As insect rearing and laboratory facility, S. exigua completed its development for 32.2 days at 25℃. Egg hatching, pupation, and adult eclosion showed 80, 86.6, and 90%. Two pesticides, fluxametamide and hydramethylnon showed high susceptibility against S. exigua larvae by spraying and dipping methods. As physical containment facility, male adults, which were artificially released from laboratory, did not capture in delta trap installed sex pheromone lure during winter season. Based on these results, QPRF will be used for study on alien insect species.

농림축산검역본부는 해외해충 연구를 위해 밀폐능력을 갖춘 검역해충실험동을 구축하였다. 연구실험실로써 검역해충실험동의 적합성을 확인하기 위해 파밤나방을 이용하여 해충사육, 연구, 밀폐기능을 검증하였다. 해충사육 기능검증에서 한 세대의 파밤나방 완성에 필요한 온도별 생활사는 25℃에서 32.2일이 소요되었으며, 알 부화율은 80%, 유충 용화율은 86.6%, 번데기 우화율은 90%로 조사되었다. 연구기능 검증에서 살포법과 침지법을 이용하여 2종의 살충제(풀룩사메타마이드와 히드라메칠론)에 대한 고도의 유충 감수성을 확인하였다. 밀폐기능 검증에서는 겨울 동안 수행된 검역해충실험동의 유출시험에서 외부에 설치된 성페로몬 트랩에 파밤나방 수컷성충이 포획되지 않아 성충 유출은 없었다. 본 연구결과를 바탕으로 해외해충에 대한 다양한 연구가 검역해충실험동에서 가능할 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 농림축산검역본부의 농림축산검역검사기술개발사업 "외래 식물해충 안전 연구시스템 개발을 위한 유출 위험도 평가 및 검증(F-1543086-2017-18-01)"에 의해 수행되었다. 또한 본 연구의 기초실험에 도움을 준 성세하 연구원에게 감사드립니다.

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