The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea

  • Kwon, Oh-Kyu (Department of Molecular Biology, Sejong University) ;
  • Jeong, A-Ram (Department of Molecular Biology, Sejong University) ;
  • Jeong, Yong-Jik (Department of Bioresources Engineering, Sejong University) ;
  • Kim, Young-Ah (Department of Bioresources Engineering, Sejong University) ;
  • Shim, Jaekyung (Department of Molecular Biology, Sejong University) ;
  • Jang, Yoon Jeong (Department of Plant Science and Technology, Chung-Ang University) ;
  • Lee, Gung Pyo (Department of Plant Science and Technology, Chung-Ang University) ;
  • Park, Chang-Jin (Department of Molecular Biology, Sejong University)
  • 투고 : 2021.02.05
  • 심사 : 2021.05.24
  • 발행 : 2021.08.01
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초록

Alternaria leaf blight is one of the most common diseases in watermelon worldwide. In Korea, however, the Alternaria species causing the watermelon leaf blight have not been investigated thoroughly. A total of 16 Alternaria isolates was recovered from diseased watermelon leaves with leaf blight symptoms, which were collected from 14 fields in Korea. Analysis of internal transcribed spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and RNA polymerase II second largest subunit (RPB2) were not competent to differentiate the Alternaria isolates. On the contrary, analysis of amplicon size of the histone H3 (HIS3) gene successfully differentiated the isolates into three Alternaria subgroups, and further sequence analysis of them identified three Alternaria spp. Alternaria tenuissima, A. gaisen, and A. alternata. Representative Alternaria isolates from three species induced dark brown leaf spot lesions on detached watermelon leaves, indicating that A. tenuissima, A. gaisen, and A. alternata are all causal agents of Alternaria leaf blight. Our results indicate that the Alternaria species associated watermelon leaf blight in Korea is more complex than reported previously. This is the first report regarding the population structure of Alternaria species causing watermelon leaf blight in Korea.

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과제정보

This study was carried out with the support of the ''Cooperative Research Program for Agriculture Science & Technology Development [Project No. PJ01421303 (C.J.P) and No. PJ01421302 (G.P.L)]'' Rural Development Administration, Republic of Korea.

참고문헌

  1. Akhtar, N., Hafeez, R. and Awan, Z. A. 2014. First report of rice leaf spot by Alternaria gaisen from Pakistan. Plant Dis. 98:1440. https://doi.org/10.1094/PDIS-05-14-0477-PDN
  2. Andersen, B., Kroger, E. and Roberts, R. G. 2001. Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycol. Res. 105:291-299. https://doi.org/10.1017/S0953756201003446
  3. Baudry, A., Morzieres, J. P. and Larue, P. 1993. First report of Japanese pear black spot caused by Alternaria kikuchiana in France. Plant Dis. 77:428.
  4. Berbee, M. L., Pirseyedi, M. and Hubbard, S. 1999. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia 91:964-977. https://doi.org/10.2307/3761627
  5. Bessadat, N., Hamon, B., Bataille-Simoneau, N., Mabrouk, K. and Simoneau, P. 2020. Alternaria telliensis sp. nov., a new species isolated from Solanaceae in Algeria. Phytotaxa 440:89-100. https://doi.org/10.11646/phytotaxa.440.2.1
  6. Blagojevic, J. D., Vukojevic, J. B. and Ivanovic, Z. S. 2020. Occurrence and characterization of Alternaria species associated with leaf spot disease in rapeseed in Serbia. Plant Pathol. 69:883-900. https://doi.org/10.1111/ppa.13168
  7. Burdman, S. and Walcott, R. 2012. Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry. Mol. Plant Pathol. 13:805-815. https://doi.org/10.1111/j.1364-3703.2012.00810.x
  8. Chen, D.-H. and Ronald, P. C. 1999. A rapid DNA minipreparation method suitable for AFLP and other PCR applications. Plant Mol. Biol. Rep. 17:53-57. https://doi.org/10.1023/A:1007585532036
  9. Chopra, B. L., Jhooty, J. S. and Bajaj, K. L. 1974. Biochemical differences between two varieties of watermelon resistant and susceptible to Alternaria cucumerina. J. Phytopathol. 79:47-52. https://doi.org/10.1111/j.1439-0434.1974.tb02688.x
  10. Food and Agriculture Organization of the United Nations. 2018. Food and Agriculture Organization of the United Nations Online Database. URL http://www.fao.org/faostat/en/#data [5 February 2021].
  11. Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61:1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  12. Iacomi-Vasilescu, B., Avenot, H., Bataille-Simoneau, N., Laurent, E., Guenard, M. and Simoneau, P. 2004. In vitro fungicide sensitivity of Alternaria species pathogenic to crucifers and identification of Alternaria brassicicola field isolates highly resistant to both dicarboximides and phenylpyrroles. Crop Prot. 23:481-488. https://doi.org/10.1016/j.cropro.2003.10.003
  13. Ismail, S. I. and Abd Razak, N. F. 2021. First report of Nigrospora sphaerica causing leaf spot on watermelon (Citrullus lanatus) in Malaysia. Plant Dis. 105:488. https://doi.org/10.1094/PDIS-07-20-1491-PDN
  14. Jackson, C. R. and Weber, G. F. 1959. Morphology and taxonomy of alternaria cucumerina. Mycologia 51:401-408. https://doi.org/10.2307/3756059
  15. Kang, J.-C., Crous, P. W. and Schoch, C. L. 2001. Species concepts in the Cylindrocladium floridanum and Cy. spathiphylli complexes (Hypocreaceae) based on multi-allelic sequence data, sexual compatibility and morphology. Syst. Appl. Microbiol. 24:206-217. https://doi.org/10.1078/0723-2020-00026
  16. Kim, W. G., Cho, W. D., Lee, Y. H. and Yu, S. H. 1994. Leaf blight of watermelon caused by Alternaria cucumerina. Korean J. Plant Pathol. 10:245-248.
  17. Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33:1870-1874. https://doi.org/10.1093/molbev/msw054
  18. Lawrence, D. P., Rotondo, F. and Gannibal, P. B. 2016. Biodiversity and taxonomy of the pleomorphic genus Alternaria. Mycol. Prog. 15:3. https://doi.org/10.1007/s11557-015-1144-x
  19. Liu, Y. J., Whelen, S. and Hall, B. D. 1999. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Mol. Biol. Evol. 16:1799-1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  20. Liu, Z. H., LuBin, Zhao, T.-C., Yang, H., Liu, F., Sun, J. and Han, X. Y. 2010. Biological characteristics of the pathogenic fungus causing leaf spot disease of watermelon. J. Shenyang Agric. Univ. 41:161-164. https://doi.org/10.3969/j.issn.1000-1700.2010.02.007
  21. Ma, G., Bao, S., Zhao, J., Sui, Y. and Wu, X. 2021. Morphological and molecular characterization of Alternaria species causing leaf blight on watermelon in China. Plant Dis. 105:60-70. https://doi.org/10.1094/PDIS-01-20-0130-RE
  22. Nishikawa, J. and Nakashima, C. 2019. Morphological and molecular characterization of the strawberry black leaf spot pathogen referred to as the strawberry pathotype of Alternaria alternata. Mycoscience 60:1-9. https://doi.org/10.1016/j.myc.2018.05.003
  23. Noh, J., Kim, J.-H., Lim, J. H., Kim, T. B., Seong, M. H., Jung, G. T., Kim, J. M., Cheong, S.-S., Oh, N. K. and Lee, W.-H. 2014. Occurrence of diseases and case of clinical diagnosis on watermelon in South Korea, 2008-2012. Res. Plant Dis. 20:8-14. https://doi.org/10.5423/RPD.2014.20.1.008
  24. Perveen, S., Akhtar, N. and Nayab, M. 2018. Alternaria gaisen: a new pathogen causing leaf spot of Gerbera jamesonii from Pakistan. Mycopath 16:7-10.
  25. Pryor, B. M. and Michailides, T. J. 2002. Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with alternaria late blight of pistachio. Phytopathology 92:406-416. https://doi.org/10.1094/PHYTO.2002.92.4.406
  26. Simmons, E. G. 2007. Alternaria: an identification manual. CBS Biodiversity Series 6. CBS Fungal Biodiversity Centre, Utrecht, The Netherlands. 775 pp.
  27. Simmons, E. G. and Roberts, R. G. 1993. Alternaria themes and variations (73). Mycotaxon 48:109-140.
  28. Tamura, K. and Nei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10:512-526.
  29. Tian, Y., Qiu, C. D., Zhang, Y. Y. and Liu, Z. Y. 2020. First report of Alternaria gaisen causing leaf blight on wintersweet (Chimonanthus praecox) in China. Plant Dis. 104:977.
  30. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols a guide to methods and applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
  31. Woudenberg, J. H. C., Groenewald, J. Z., Binder, M. and Crous, P. W. 2013. Alternaria redefined. Stud. Mycol. 75:171-212. https://doi.org/10.3114/sim0015
  32. Wu, S., Wang, X., Reddy, U., Sun, H., Bao, K., Gao, L., Mao, L., Patel, T., Ortiz, C., Abburi, V. L., Nimmakayala, P., Branham, S., Wechter, P., Massey, L., Ling, K.-S., Kousik, C., Hammar, S. A., Tadmor, Y., Portnoy, V., Gur, A., Katzir, N., Guner, N., Davis, A., Hernandez, A. G., Wright, C. L., McGregor, C., Jarret, R., Zhang, X., Xu, Y., Wehner, T. C., Grumet, R., Levi, A. and Fei, Z. 2019. Genome of 'Charleston Gray', the principal American watermelon cultivar, and genetic characterization of 1,365 accessions in the U.S. National Plant Germplasm System watermelon collection. Plant Biotechnol. J. 17:2246-2258. https://doi.org/10.1111/pbi.13136
  33. Zhao, J., Bao, S., Ma, G. and Wu, X. 2016a. Characterization of Alternaria species associated with muskmelon foliar diseases in Beijing municipality of China. J. Gen. Plant Pathol. 82:29-32. https://doi.org/10.1007/s10327-015-0631-x
  34. Zhao, J., Bao, S. W., Ma, G. P. and Wu, X. H. 2016b. Characterization of Alternaria species associated with watermelon leaf blight in Beijing municipality of China. J. Plant Pathol. 98:135-138.
  35. Zheng, H. H., Zhao, J., Wang, T. Y. and Wu, X. H. 2015. Characterization of Alternaria species associated with potato foliar diseases in China. Plant Pathol. 64:425-433. https://doi.org/10.1111/ppa.12274
  36. Zhou, X. G. and Everts, K. L. 2008. First report of Alternaria alternata f. sp. cucurbitae causing alternaria leaf spot of melon in the mid-atlantic region of the United States. Plant Dis. 92:652. https://doi.org/10.1094/PDIS-92-4-0652B