The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Effect of Salicylic Acid Formulations on Induced Plant Defense against Cassava Anthracnose Disease

  • Sangpueak, Rungthip (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Phansak, Piyaporn (Division of Biology, Faculty of Science, Nakhon Phanom University) ;
  • Thumanu, Kanjana (Synchrotron Light Research Institute) ;
  • Siriwong, Supatcharee (Synchrotron Light Research Institute) ;
  • Wongkaew, Sopone (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Buensanteai, Natthiya (School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology)
  • Received : 2021.02.02
  • Accepted : 2021.06.29
  • Published : 2021.08.01
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Abstract

This study was to investigate defense mechanisms on cassava induced by salicylic acid formulation (SA) against anthracnose disease. Our results indicated that the SA could reduce anthracnose severity in cassava plants up to 33.3% under the greenhouse condition. The 𝛽-1,3-glucanase and chitinase enzyme activities were significantly increased at 24 hours after inoculation (HAI) and decrease at 48 HAI after Colletotrichum gloeosporioides challenge inoculation, respectively, for cassava treated with SA formulation. Synchrotron radiation-based Fourier-transform infrared microspectroscopy spectra revealed changes of the C=H stretching vibration (3,000-2,800 cm-1), pectin (1,740-1,700 cm-1), amide I protein (1,700-1,600 cm-1), amide II protein (1,600-1,500 cm-1), lignin (1,515 cm-1) as well as mainly C-O-C of polysaccharides (1,300-1,100 cm-1) in the leaf epidermal and mesophyll tissues treated with SA formulations, compared to those treated with fungicide carbendazim and distilled water after the challenged inoculation with C. gloeosporioides. The results indicate that biochemical changes in cassava leaf treated with SA played an important role in the enhancement of structural and chemical defense mechanisms leading to reduced anthracnose severity.

Keywords

Acknowledgement

The authors wish to express grateful the research assistant of Plant Pathology and Biopesticide Laboratory, Suranaree University of Technology, for the technical assistance, and Synchrotron Light Research Institute (Public Organization) for providing the SR-FTIR instruments and beam times. This work was supported by the Research and Researcher for Industries (RRi), Thailand Research Funds [grant number PHD59I0084] to Miss Rungthip Sangpueak.

References

  1. Alonso-Simon, A., Garcia-Angulo, P., Melida, H., Encina, A., Alvarez, J. M. and Acebes, J. L. 2011. The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors. Plant Signal. Behav. 6:1104-1110. https://doi.org/10.4161/psb.6.8.15793
  2. Baker, M. J., Trevisan, J., Bassan, P., Bhargava, R., Butler, H. J., Dorling, K. M., Fielden, P. R., Fogarty, S. W., Fullwood, N. J., Heys, K. A., Hughes, C., Lasch, P., Martin-Hirsch, P. L., Obinaju, B., Sockalingum, G. D., Sule-Suso, J., Strong, R. J., Walsh, M. J., Wood, B. R., Gardner, P. and Martin, F. L. 2014. Using Fourier transform IR spectroscopy to analyze biological materials. Nat. Protoc. 9:1771-1791. https://doi.org/10.1038/nprot.2014.110
  3. El-kereamy, A., El-sharkawy, I., Ramamoorthy, R., Taheri, A., Errampalli, D., Kumar, P. and Jayasankar, S. 2011. Prunus domestica pathogenesis-related protein-5 activates the defense response pathway and enhances the resistance to fungal infection. PLoS ONE 6:e17973. https://doi.org/10.1371/journal.pone.0017973
  4. Gao, Q. M., Zhu, S., Kachroo, P. and Kachroo, A. 2015. Signal regulators of systemic acquired resistance. Front. Plant Sci. 6:228. https://doi.org/10.3389/fpls.2015.00228
  5. Gogbeu, S. J., Sekou, D., Kouakou, K. J., Dogbo, D. O. and Bekro, Y.-A. 2015. Improvement of cassava resistance to Colletotrichum gloeosporioides by salicylic acid, phosphorous acid and fungicide Sumi 8. Int. J. Curr. Microbiol. App. Sci. 4:854-865.
  6. Gupta, P., Ravi, I. and Sharma, V. 2012. Induction of β-1,3-glucanase and chitinase activity in the defense response of Eruca sativa plants against the fungal pathogen Alternaria brassicicola. J. Plant Interact. 8:155-161. https://doi.org/10.1080/17429145.2012.679705
  7. Jayaraj, J., Muthukrishnan, S., Liang, G. H. and Velazhahan, R. 2004. Jasmonic acid and salicylic acid induce accumulation of β-1,3-glucanase and thaumatin-like proteins in wheat and enhance resistance against Stagonospora nodorum. Biol. Plant. 48:425-430. https://doi.org/10.1023/B:BIOP.0000041097.03177.2d
  8. Jendoubi, W., Harbaoui, K. and Hamada, W. 2015. Salicylic acidinduced resistance against Fusarium oxysporum f. sp. radicislycopercisi in hydroponic grown tomato plants. J. New Sci. 21:985-995.
  9. Koo, Y. M., Heo, A. Y. and Choi, H. W. 2020. Salicylic acid as a safe plant protector and growth regulator. Plant Pathol. J. 36:1-10. https://doi.org/10.5423/PPJ.RW.12.2019.0295
  10. Liu, X., Shi, T., Li, B., Cai, J., Li, C. and Huang, G. 2019. Colletotrichum species associated with cassava anthracnose in China. J. Phytopathol. 167:1-9. https://doi.org/10.1111/jph.12765
  11. Lu, H. 2009. Dissection of salicylic acid-mediated defense signaling networks. Plant Signal. Behav. 4:713-717. https://doi.org/10.4161/psb.4.8.9173
  12. Lu, H., Greenberg, J. T. and Holuigue, L. 2016. Editorial: salicylic acid signaling networks. Front. Plant Sci. 7:238.
  13. Nair, A. B. and Umamaheswaran, K. 2016. Enzymatic responses to SriLankan cassava mosaic virus infection in cassava plants after grafting. Int. J. Appl. Pure Sci. Agric. 2:165-170.
  14. Ong, S. and Cruz, F. C. S. 2016. Effect of exogenous application of salicylic acid on the severity of tomato leaf curl disease. J. Int. Soc. Southeast Asian Agric. Sci. 22:137-145.
  15. Prakongkha, I., Sompong, M., Wongkaew, S., Athinuwat, D. and Buensanteai, N. 2013. Foliar application of systemic acquired resistance (SAR) inducers for controlling grape anthracnose caused by Sphaceloma ampelinum de Bary in Thailand. Afr. J. Biotechnol. 12:5148-5156. https://doi.org/10.5897/AJB12.2650
  16. Sangpueak, R., Phansak, P. and Buensanteai, N. 2018. Morphological and molecular identification of Colletotrichum species associated with cassava anthracnose in Thailand. J. Phytopathol. 166:129-142 https://doi.org/10.1111/jph.12669
  17. Santos, I. S., Da Cunha, M., Machado, O. L. T. and Gomes, V. M. 2004. A chitinase from Adenanthera pavonina L. seeds: purification, characterisation and immunolocalisation. Plant Sci. 167:1203-1210. https://doi.org/10.1016/j.plantsci.2004.04.021
  18. Thakur, M. and Sohal, B. S. 2013. Role of elicitors in inducing resistance in plants against pathogen infection: a review. ISRN Biochem. 2013:762412. https://doi.org/10.1155/2013/762412
  19. Thanh, T. L., Thumanu, K., Wongkaew, S., Boonkerd, N., Teaumroong, N., Phansak, P. and Buensanteai, N. 2017. Salicylic acid-induced accumulation of biochemical components associated with resistance against Xanthomonas oryzae pv. oryzae in rice. J. Plant Interact. 12:108-120. https://doi.org/10.1080/17429145.2017.1291859
  20. Wang, H., Kashyap, Y. and Sawhney, K. 2016. From synchrotron radiation to lab source: advanced speckle-based X-ray imaging using abrasive paper. Sci. Rep. 6:20476. https://doi.org/10.1038/srep20476
  21. War, A. R., Paulraj, M. G., War, M. Y. and Ignacimuthu, S. 2011. Role of salicylic acid in induction of plant defense system in chickpea (Cicer arietinum L.). Plant Signal. Behav. 6:1787-1792. https://doi.org/10.4161/psb.6.11.17685
  22. William, M. N. M., Mbega, E. R. and Mabagala, R. B. 2012. An outbreak of anthracnose caused by Colletotrichum gloesporioides f. sp. manihotis in Cassava in North Western Tanzania. Am. J. Plant Sci. 3:596-598. https://doi.org/10.4236/ajps.2012.35072
  23. Zur, I., Golebiowska, G., Dubas, E., Golemiec, E., Matusikova, I., Libantova, J. and Moravcikova, J. 2013. β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale. Biologia 68:241-248. https://doi.org/10.2478/s11756-013-0001-0