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Effect of the Internal Shape of eDNA Filter Case made by the PBF method Metal 3D Printer on Water Sampling Performance

PBF 방식 금속 3D프린터로 제작된 환경DNA 필터 케이스의 내부 형상이 포집 성능에 미치는 영향

  • Lee, Seung-Min (School of Mechanical Engineering, Gyeongsang National University) ;
  • Park, Se-Hyun (School of Mechanical Engineering, Gyeongsang National University) ;
  • Kwak, Ihn-Sil (Department of Ocean Intergrated Science, Chonnam National University) ;
  • Kim, Hyoung-Ho (School of Mechanical Engineering, Gyeongsang National University) ;
  • Kwak, Tae-Soo (School of Mechanical Engineering, Gyeongsang National University)
  • 이승민 (경상국립대학교 기계공학부) ;
  • 박세현 (경상국립대학교 기계공학부) ;
  • 곽인실 (전남대학교 해양융합과학과) ;
  • 김형호 (경상국립대학교 기계공학부) ;
  • 곽태수 (경상국립대학교 기계공학부)
  • Received : 2021.05.25
  • Accepted : 2021.07.06
  • Published : 2021.08.31

Abstract

This study focuses on designing a filter case using a water pump for application in eDNA filtering systems. Filter cases, channel type and net type were designed based on the flow field and made using a 3D printer for metal. Flow analysis was conducted for each filter case, and the results were consistent with the pressure experiment results. Furthermore, the water sampling performance test showed that the channel-type filter case exhibited a high flow rate and low pressure through the filter. The eDNA extraction experiment showed that the channel type exhibited improved capture ability compared to the net type.

Keywords

Acknowledgement

이 논문은 2020년도 경상국립대학교 교원 연구활성화 지원 사업의 예산지원으로 수행되었음.

References

  1. Ficetola, G. F., Miaud, C., Pompanon, F., and Taberlet, P."Species detection using environmental DNA from water samples," Biology letters, Vol. 4 No. 4, pp. 423-425, 2008. https://doi.org/10.1098/rsbl.2008.0118
  2. Thomsen, P. F., and Willerslev, E., "Environmental DNA-An emerging tool in conservation for monitoring past and present biodiversity," Biological conservation, Vol. 183, pp. 4-18, 2015. https://doi.org/10.1016/j.biocon.2014.11.019
  3. Shaw, J. L., Clarke, L. J., Wedderburn, S. D., Barnes, T. C., Weyrich, L. S., and Cooper, A., "Comparison of environmental DNA metabarcoding and conventional fish survey methods in a river system," Biological Conservation, Vol. 197, pp. 131-138, 2016. https://doi.org/10.1016/j.biocon.2016.03.010
  4. Boussarie, G., Bakker, J., Wangensteen, O. S., Mariani, S., Bonnin, L., Juhel, J. B., and Vigliola, L.,"Environmental DNA illuminates the dark diversity of sharks," Science advances, Vol. 4, No. 5, eaap9661, 2018. https://doi.org/10.1126/sciadv.aap9661
  5. Jo, H. B., Choi., B. H., Park, K. Y., Kim. W. S. and Kwak, I. S., "First Gut Content Analysis of 4th Instar Midge Larvae (Diptera: Chronomidae) In Large-Scale Weirs Using a DNA Meta-Barcoding Approach," International Journal of Environmental Research and Public Health, Vol. 17, pp. 2856, 2020. https://doi.org/10.3390/ijerph17082856
  6. Rees, H. C., Maddison, B. C., Middleditch, D. J., Patmore, J. R., and Gough, K. C.,"The detection of aquatic animal species using environmental DNA-a review of eDNA as a survey tool in ecology". Journal of Applied Ecology, Vol. 51, No. 5, pp. 1450-1459, 2014. https://doi.org/10.1111/1365-2664.12306
  7. Bohmann, K., Evans, A., Gilbert, M. T. P., Carvalho, G. R., Creer, S., Knapp, M., and De Bruyn, M.,"Environmental DNA for wildlife biology and biodiversity monitoring," Trends in ecology and evolution, Vol. 29, No. 6, pp. 358-367, 2014. https://doi.org/10.1016/j.tree.2014.04.003
  8. Lim, N. K., Tay, Y. C., Srivathsan, A., Tan, J. W., Kwik, J. T., Baloglu, B., and Yeo, D. C., "Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities," Royal Society Open Science, Vol. 3, No. 11, rsos.160635, 2016.
  9. Bass, D., Stentiford, G. D., Littlewood, D. T. J., and Hartikainen, H., "Diverse applications of environmental DNA methods in parasitology," Trends in Parasitology, Vol. 31, No. 10, pp. 499-513, 2015. https://doi.org/10.1016/j.pt.2015.06.013
  10. Gunter G., Ute M. and Michael S., "Water Lice and Other Macroinvertebrates in Drinking Water Pipes: Diversity, Abundance and Health Risk," Water, Vol. 13, No. 3, 276, 2021. https://doi.org/10.3390/w13030276
  11. Miya, M., Y. Sato, T. Fukunaga, T. Sado, J.Y. Poulsen, K. Sato and W. Iwasaki., "MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species," Royal Society open science, Vol. 2, No. 7, 150088, 2015. https://doi.org/10.1098/rsos.150088
  12. Osathanunkul, M. and Minamoto, T., "A molecular survey based on eDNA to assess the presence of a clown featherback(Chitala ornata) in a confined environment," PeerJ, 8: e10338. 2020. https://doi.org/10.7717/peerj.10338
  13. Sanches, T.M and A.D. Schreier.,"Optimizing an eDNA protocol for estuarine environments: Balancing sensitivity cost and time," Plos one, Vol. 15 No. 5, e0233522, 2020. https://doi.org/10.1371/journal.pone.0233522
  14. Schabacker, J. C., Amish, S. J., Ellis, B. K., Gardner, B., Miller, D. L., Rutledge, E. A. and Luikart, G., "Increased eDNA detection sensitivity using a novel high-volume water sampling method," Environmental DNA, Vol. 2, No. 2, pp. 244-251, 2020. https://doi.org/10.1002/edn3.63
  15. Choi, K. C. and Cho, J. U.,"A Study on the Efficient Flow Analysis due to Valve Shape", Journal of the Korean Society of Manufacturing Process Engineers, Vol. 19, No. 6, pp. 17-22, 2020. https://doi.org/10.14775/ksmpe.2020.19.06.017