대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)

  • 제34권3호
  • /
  • Pages.15-28
  • /
  • 2021
  • /
  • 1229-4292(pISSN)
  • /
  • 2508-3619(eISSN)
대한한방부인과학회 (The Society of Korean Medicine Obstetrics and Gynecology)
권호 표지
DOI QR코드

DOI QR Code

맥문동 물 추출물의 선천면역 활성과 염증억제 효과

The Effects of Liriopis Tuber Water Extract on Innate Immune Activation and Anti-Inflammation

  • 강누리 (경희대학교 대학원 임상한의학과) ;
  • 황덕상 (경희대학교 한의과대학 부인과교실) ;
  • 이진무 (경희대학교 한의과대학 부인과교실) ;
  • 이창훈 (경희대학교 한의과대학 부인과교실) ;
  • 장준복 (경희대학교 한의과대학 부인과교실)
  • Kang, Nu-Ri (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Hwang, Deok-Sang (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Jin-Moo (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Chang-Hoon (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University) ;
  • Jang, Jun-Bock (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University)
  • 투고 : 2021.07.15
  • 심사 : 2021.08.27
  • 발행 : 2021.08.27
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: This study was designed to examine the anti-cancer activity by innate immunomodulating and anti-inflammatory effects of liriopis tuber water extract (LPE). Methods: Cell cytotoxicity was tested with 4T1 mouse mammary carcinoma cells, spleen cells, macrophage, and RAW264.7 cells. To investigate innate immunomodulating effects of LPE on macrophage, we measured tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). To investigate innate immunomodulating effects of LPE on RAW264.7 cell, we measured TNF-α, interleukin-6 (IL-6). In addition, TNF-α and nitric oxide (NO) induced by lipopolysaccharide (LPS) were measured after treating with LPE to observe innate immunomodulating effect of LPE on RAW264.7 cell. Also, mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis. Results: In an in vitro cytotoxicity analysis, LPE affected tumor cell growth above specific concentration. As compared with the control group, the production of TNF-α, IL-12, and IL-10 were increased in macrophage. As compared with the control group, the production of TNF-α and IL-6 were increased in RAW 264.7 cell. The expression of TNF-α and NO induced by LPS after treating LPE was decreased. In addition, treatment of RAW 264.7 cell with LPE increased the phosphorylation levels of p-extracellular signal-regulated kinase (p-ERK), p-Jun N-terminal kinase (p-JNK), and p-p38. Conclusions: LPE might have impact on the anti-cancer effect by activation of innate immune system and inflammation control.

키워드

참고문헌

  1. Oriental Gynecology Textbook Compilation Committee. Korean Medicine Obstetric & Gynecology I. Seoul:Jeongdam. 2007:282-310.
  2. Kim JH, et al. Review on Clinical Study of Oriental Medical Treatment for Cancer in Korea. J Acupunct Res. 2012;29(3):19-28.
  3. Jiang MH, Zhu L, Jiang JG. Immunoregulatory actions of polysaccharides from Chinese herbal medicine. Expert Opin Ther Targets. 2010;14(12):1367-402. https://doi.org/10.1517/14728222.2010.531010
  4. Bae SK, et al. Modulatory Effects of Cytokine Release in Immune Response of RAW 264.7 and TK-1. J Int Korean Med. 2018;39(6):1244-55. https://doi.org/10.22246/jikm.2018.39.6.1244
  5. Song YC, et al. A Potential Herbal Adjuvant Combined With a Peptide-Based Vaccine Acts Against HPV-Related Tumors Through Enhancing Effector and Memory T-Cell Immune Responses. Frontiers in immunology. 2020;11(62):1-15. https://doi.org/10.3389/fimmu.2020.00001
  6. Heo J. Dongeuibogam. Gyeongnam: Dongeuibogam Publishing Co. 2004:2149.
  7. National College of Korean Medicine Textbook Compilation Committee. Herbology. Seoul:Youglimsa. 2008:644-5.
  8. Im JG, et al. Dietary Effect of Liriopis Tuber Water Extracts on the Level of Blood Glucose and Serum Cholesterol in Streptozotocin-Induced Diabetic Rat. J East Asian Soc Dietary Life. 2005; 15(1):20-8.
  9. Lee SK, Park JH, Kim YT. A Study on the Antioxidation and Antimicrobial Effect of "Megmoondong(Liriope platyphylla Wang et Tang)" Water Extracts. Korean J Food & Nutr. 2009;22(2):279-85.
  10. Roh SS, et al. Studies of Anti-inflammation of Liriopis Tuber to Autoimmunune Diabetes in NOD Mice. Korean J. Oriental Physiology & Pathology. 2008;22(4):766-70.
  11. An JY. The Effect of Liriopis Tuber on Liver Function Activity. Department of Pharmacy, Graduate School, Taegu-Hyosung Catholic University. 2000.
  12. Kang YG, Lee TH. Liriopis Tuber Improves Stress-Induced Memorial Impairments in Rats. Kor J Herbology. 2006;21(2):63-75.
  13. Baek NI, et al. Cytotoxicity of Steroid-Saponins from the Tuber of Liriope Platyphylla W.T. Agricultural Chemistry and Biotechnology. 1998;41(5):390-4.
  14. Li H, et al. DT-13, a Saponin Monomer of Dwarf Lilyturf Tuber, Induces Autophagy and Potentiates Anti-Cancer Effect of Nutrient Deprivation. Eur J Pharmacol. 2016;781:164-72. https://doi.org/10.1016/j.ejphar.2016.04.016
  15. Li S, et al. The Saponin Monomer of Dwarf Lilyturf Tuber, DT-13, Reduces Human Breast Cancer Cell Adhesion and Migration during Hypoxia via Regulation of Tissue Factor. Biol Pharm Bull. 2010;33(7):1192-8. https://doi.org/10.1248/bpb.33.1192
  16. Renping Z, et al. The Saponin Monomer of Dwarf Lilyturf Tuber, DT-13, Inhibits Angiogenesis Under Hypoxia and Normoxia via Multi-Targeting Activity. Oncol Rep. 2013;29(4):1379-86. https://doi.org/10.3892/or.2013.2272
  17. Renping Z, et al. DT-13, a Saponin of Dwarf Lilyturf Tuber, Exhibits Anti-Cancer Activity by Down-Regulating C-C Chemokine Receptor Type 5 and Vascular Endothelial Growth Factor in MDA-MB-435 Cells. Chin J Nat Med. 2014;12(1):24-9. https://doi.org/10.1016/S1875-5364(14)60005-4
  18. Hongzhi D, et al. DT-13 Inhibits Cancer Cell Migration by Regulating NMIIA Indirectly in the Tumor Microenvironment. Oncol Rep. 2016;36(2):721-8. https://doi.org/10.3892/or.2016.4890
  19. Adlercreutz H. Phytoestrogens: Epidemiology and a Possible Role in Cancer Protection. Environmental Health Perspectives. 1995;103 Suppl 7(Suppl 7):103-12.
  20. Yamamoto, et al. Soy, Isoflavones, and Breast Cancer Risk in Japan. J of the National Cancer Institute. 2003;95(12):906-13. https://doi.org/10.1093/jnci/95.12.906
  21. Suh DS, et al. Apoptotic Effect of NV-196, an Isoflavone Derivative, in Epithelial Ovarian Cancer Cells. Korean J Obstet Gynecol. 2011;54(2):86-92. https://doi.org/10.5468/KJOG.2011.54.2.86
  22. Bauman JE, Ferris RL. Integrating Novel Therapeutic Monoclonal Antibodies into the Management of Head and Neck Cancer. Cancer. 2014;120(5):624-32. https://doi.org/10.1002/cncr.28380
  23. Cho JK. Oriental Clinical Oncology. 1st ed. Daejeon:Jumin Publishing Co. 2001:9-15.
  24. Kim DH, et al. Hwangjenaegyeong somunhaeseog-Ha. 1st ed. Seoul:Uiseongdang. 2001:746.
  25. Chang WS. Hwalbeobgiyo. Seoul:Daeseong Publishing Co. 1986:129-30.
  26. Kim IG, et al. Mixture Bombyx mori L. and Liriopis seu Ophiopogonis Tuber effects on T cells in Thymus, Lymph Nodes. Kor J Herbol. 2018;33(5):47-52. https://doi.org/10.6116/kjh.2018.33.5.47
  27. William EP. Fundamental Immunology. 5th ed. USA:Lippincott Williams & Wilkins. 2003:20.
  28. Ha ES, et al. Anti-metastatic Activity of Glycoprotein Fractionated from Acanthopanax Senticosus, Involvement of NK-cell and Macrophage Activation. Arch Pharm Res. 2004;27(2):217-24. https://doi.org/10.1007/BF02980109
  29. Abbas L, et al. Cellular and Molecular Immunology. 5th ed. Netherlands:Elsevier Health Sciences. 2003:247-63.
  30. Kurebayashi J, et al. Medroxyprogesterone Acetate Inhibits Interleukin 6 Secretion from KPL-4 Human Breast Cancer Cells Both in vitro and in vivo: a Possible Mechanism of the Anticachectic Effect. Br J Cancer. 1999;79(3/4):631-6. https://doi.org/10.1038/sj.bjc.6690099
  31. Gee K, et al. Differential Regulation of CD44 Expression by Lipopolysaccharide (LPS) and TNF-alpha in Human Monocytic Cells: Distinct Involvement of C-Jun N-terminal Kinase in LPS-Induced CD44 Expression. J Immunol. 2002;169(10):5660-72. https://doi.org/10.4049/jimmunol.169.10.5660
  32. Guha M, Mackman N. LPS Induction of Gene Expression in Human Monocytes. Cell Signal. 2001;13(2):85-94. https://doi.org/10.1016/S0898-6568(00)00149-2
  33. Takeda K, Kaisho T, Akira S. Toll-Like Receptors. Annu Rev Immunol. 2003;21:335-76. https://doi.org/10.1146/annurev.immunol.21.120601.141126
  34. Paik YH, et al. Toll-Like Receptor 4 Mediates Inflammatory Signaling by Bacterial Lipopolysaccharide in Human Hepatic Stellate Cells. Hepatology. 2003;37(5):979-82. https://doi.org/10.1053/jhep.2003.50224
  35. Lee AK, et al. Inhibition of Lipopolysaccharide-Inducible Nitric Oxide Synthase, TNF-α and COX-2 Expression by Sauchinone Effects on I-κBα Phosphorylation, C/EBP and AP-1 Activation. British journal of pharmacology. 2003;139(1):11-20. https://doi.org/10.1038/sj.bjp.0705231
  36. Delgado AV, McManus AT, Chambers JP. Production of Tumor Necrosis Factor-alpha, Interleukin 1-beta, Interleukin 2, and Interleukin 6 by Rat Leukocyte Subpopulations after Exposure to Substance P. Neuropeptides. 2003;37(6):355-61. https://doi.org/10.1016/j.npep.2003.09.005
  37. Aderem A, Ulevitch RJ. Toll-like Receptors in the Induction of the Innate Immune Response. Nature. 2000;406(6797):782-7. https://doi.org/10.1038/35021228
  38. Rosadini CV, Kagan JC. Early Innate Immune Responses to Bacterial LPS. Curr Opin Immunol. 2017;44:14-9. https://doi.org/10.1016/j.coi.2016.10.005
  39. Fukao T, Koyasu S. PI3K and Negative Regulation of TLR Signaling. Trends Immunol. 2003;24(7):358-63. https://doi.org/10.1016/S1471-4906(03)00139-X
  40. Peroval MY, et al. A Critical Role for MAPK Signaling Pathways in the Transcriptional Regulation of Toll Like Receptors. PLoS One. 2013;8(2):e51243. https://doi.org/10.1371/journal.pone.0051243
  41. Schorey JS, Cooper AM. Macrophage Signaling upon Mycobacterial Infection: the MAP Kinases Lead the Way. Cell Microbiol. 2003;5(3):133-42. https://doi.org/10.1046/j.1462-5822.2003.00263.x
  42. Gaestel M, et al. Protein Kinases as Small Molecule Inhibitor Targets in Inflammation. Curr Med Chem. 2007;14(21):2214-34. https://doi.org/10.2174/092986707781696636
  43. Medzhitov R, Preston-Hurlburt P, Janeway CA. A Human Homologue of the Drosophila Toll Protein Signals Activation of Adaptive Immunity. Nature. 1997;388(6640):394-7. https://doi.org/10.1038/41131