CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
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Optimization of the whole extract of Zarawand Mudaharaj (Aristolochia rotunda L.) root by Response Surface Methodology (RSM)

  • Ansari, MD Zakir (Dept. of Ilmul Saidla (Unani Pharmacy), Sufia Unani Medical College Hoapital & Research Centre) ;
  • Sofi, Ghulamuddin (Dept. of Ilmul Advia (Pharmacology), National Institute of Unani Medicine) ;
  • Hamiduddin, Hamiduddin (Dept. of Ilmul Saidla (Unani Pharmacy), National Institute of Unani Medicine) ;
  • Ahmad, Haqeeq (Dept. of Ilmul Advia (Pharmacology), National Institute of Unani Medicine) ;
  • Basri, Rabia (Dept. of Amraz-e-Niswan wa Ilmul Qabalat (Gynae and Obstetrics), National Institute of Unani Medicine) ;
  • Alam, Abrar (Dept. of Ilmul Advia (Pharmacology), Sufia Unani Medical College Hospital & Research Centre)
  • Received : 2021.06.05
  • Accepted : 2021.08.25
  • Published : 2021.08.31
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Abstract

The chemical constitution of a drug has been accepted as an important basis for pharmacological action in Unani medicine. Various dosage forms have been developed on this concept, such as decoctions (Joshanda), infusions (Khesanda), extract (Rub / Usara), and syrup. Zarawand Mudaharaj (ZM.) / Aristolochia rotunda L. root was subjected to extraction process using Soxhlet's apparatus by using Response Surface Methodology (RSM) to design the number of random runs of the extracts with variation in the factors of temperature, the concentration of ethanol in water, time for extraction, for optimizing and maximizing the yield concentration. The data obtained, was analyzed with regression equation and ANOVA two-way summary to interpret the interaction of the factors for yield maximization. Minitab version 18 was used to design and analyze data. Validation of the optimum conditions for maximum yield of the whole extract of ZM. Root was carried out by re-run of the extract using the optimized conditions. The maximum yield percentage thus obtained using RSM was 20.87% whereas using these optimum conditions 21.35 % yield was obtained thereby validating the method. The association between the response functions and the process variables was identified by a three-factor recorded Box-Behnken design. In the present study RSM is used because itis a cheap and affordable method to optimize maximum yield percentage which may be reliably used by researchers. The study set in the surface conditions for ZM. root extraction by the Soxhlet apparatus for maximizing the yield percentage.

Keywords

Acknowledgement

We express our thanks to Director NIUM for providing the necessary facilities for Research work.

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