The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
권호 표지
DOI QR코드

DOI QR Code

Establishment of Efficient Callus Induction System with Picloram Hormone in Lily Plants

Picloram을 이용한 효율적인 백합 캘러스 유도 체계 확립

  • 김종보 (건국대학교 생명공학과)
  • Received : 2021.08.03
  • Accepted : 2021.08.09
  • Published : 2021.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Lily is one of the most important 5 cut flowers in international flower market and lilies are distributed in Asia, Eurasia and North America. To develop a new lily cultivar, in addition to hybridization, mutation and selection methods, biotechnological techniques including tissue culture are also required. Establishment of tissue culture system is one of the requirement for the breeding program in Lily. Among many fields of plant tissue culture, establishment of regeneration system via embryogenic calluses are studied in many crops. In this study, research was carried out to decide the proper concentration of picloram which is used for the induction of embryogenic calluses. As a result, 3 different types of callused were observed after 3-4 weeks. They were CEC (compact embryogenic callus), FEC (friable embryogenic callus) and white callus type. 1.0 mg /l of picloram showed the best result for the production of embryogenic callus, however, due to its higher rate of browning in this concentration, 0.75 mg/l of picloram was selected as a proper concentration of picloram for the induction of CEC and FEC in Lily. These results can be contributed to the establishment of both regeneration system and mass propagation in lily in the future.

나리는 세계 5대 절화에 속하는 주요 절화 중의 하나이며, 아시아, 유라시아 및 북아메리카 등 다양한 지역에 서식한다. 나리 신품종개발을 위해서는 교배, 돌연변이 및 선발육종 기술 외에 조직배양 기술을 포함하는 생명공학기술 도입을 통한 신품종 육성이 필요한데 조직배양 체계확립은 나리 육종체계에 있어서 필수적인 요소 중 하나이다. 조직배양의 많은 분야 중 배발생캘러스를 이용하여 식물체 재분화 증식 체계확립이 많은 작물에서 연구 중인데 본 연구에서도 배발생캘러스 유도에 사용되는 오옥신 중 picloram을 다양한 농도로 처리하여 최적의 배발생캘러스 유도 농도를 선정하고자 실험을 수행하였다. 실험 결과, 3-4주 후부터 CEC (compact embryogenic callus), FEC (friable embryogenic callus) 및 백색캘러스 이렇게 3가지 형태 캘러스가 발생했는데 1.0 mg/l 처리구에서 CEC와 FEC 모두 높은 효율을 나타내었으나 갈변율이 높은 관계로 0.75 mg/l를 나리 배발생캘러스 유도에 적합한 최적 농도로 선정하였다. 이러한 연구결과는 향후 배발생캘러스를 이용한 나리 식물체 재분화 체계 확립 그리고 우량품종 증식체계 확립에 기여할 것이다.

Keywords

Acknowledgement

이 논문은 2018학년도 건국대학교의 연구년 교원 지원에 의하여 연구되었음.

References

  1. Seo JN, Nam HH, Lee SG, and Kanh HD, "Effect of plant growth regulators on bulblets regeneration of Lilium cernum," Korean Journal of Agricultural and Life Science 43(6): 29-33, 2009.
  2. Royal Horticultural Society (RHS), "The royal horticultural society colour chart," Royal Horticultural Society, London, U.K, 2001.
  3. De Jong PC, "Some notes on the evolution of lilies," lily year book North American Lily Society, 1974.
  4. Hwang YJ, Lucidos GJ, Ahn BJ, Ahn HG and Lim KB, "Crossing affinity of Oriental hybrid 'Siberia' and Korean native lily species," Flower Research Journal 20(4): 167-171, 2012. http://dx.doi.org/10.11623/frj.2012.20.4.167
  5. Ministry of Food, Agriculture, Forestry and Fisheries,"'Statistics of floriculture cultivation in 2015," 2016.
  6. Chandler SF and Lu CY, "Biotechnology in ornamental horticulture," In Vitro Cellular Developmental-Biology Plant 41: 591-601, 2005. http://dx.doi.org/10.1079/IVP2005.681
  7. Han IS, and Kim JB, "Establishment of a regeneration system for the production of Calla plants (Zantedeschia spp.) via embryogenic callus culture," Journal of Plant Biotechnology 46:32-36, 2019. https://doi.org/10.5010.JPB.2019.46.1.032 https://doi.org/10.5010/JPB.2019.46.1.032
  8. Kim J. B., Raemakers C. J. J. M., Jacobsen E., and Visser R. G. F, "Efficient somatic embryogenesis in Alstroemeria," Plant Cell Tissue Organ Cult. 86: 233-238, 2006. http://dx.doi.org/10.1007/s11240-006-9110-6
  9. Roh HS, and Kim JB, "Establishment of proliferation and regeneration system of PLBs in Phalaenopsis by treatments of a variety of types of medium, sucrose concentrations and anti-browning agents," Journal of Plant Biotechnology 41: 223-228, 2014. http://dx.doi.org/10.5010/JPB.2014.41.4.223
  10. Jeong JH, "Effect of scale position, chilling duration and IBA on bulblet formation in scale cuttings of Lilium hansoni," Flower Research Journal 23: 33-41, 1998.
  11. Joshi SK, and Dhar U, "In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya," Acta Physiological Plantarum 31: 833-838, 2009. http://dx.doi.org/10.1007/s11738.009.0299.y
  12. Sener O, Can E, Arslan M, and Celis N, "Effects of genotype and picloram concentrations on callus induction and plant regeneration from immature inflorescence of Spring Barley cultivars (Hordeum vulgare L.)," Biotechnology and Biotechnological Equipment 22(4): 915-920, 2008. https://dx.doi.org/10.1080/13102818.2008.10817578
  13. Murashige T, and Skoog F, "Revised medium for rapid growth and bioassays with tobacco tissue culture," Physiologia Plantarium 15: 473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Yang HR, Lee SH, and Kim JB, "Establishment of efficient Alstroemeria callus induction syste, using node culture and various hormones." The Journal of the Convergence on Culture Technology 5(1): 413-416, 2019. http://dx.doi.org/10.17703/JCCT.2019.5.1.413
  15. Park MW, Ryu SH, Lee YY, Song JM, Kim JH, Ahn YH, and Baw KW, "Callus induction and in vitro plant regeneration of Polygonatum stenophyllum Maxim," Journal of Plant Biotechnology 45: 266-272, 2018. https://dx.doi.org/10.5010/JPB.2018.45.3.266
  16. Lee YS and Ko JA, "Effect of plant growth regulators on in vitro micropropagation of colored calla lily (Zantedeschia spp.)," Korean Journal of Plant Resources 18(1): 154-160, 2005.
  17. Ko WH, Su CC, Chen CL, and Chao CP, "Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid," Plant Cell Tissue and Organ Culture 96(2): 137-141, 2009. http://dx.doi.org/10.1007/s11240.008.9469.7
  18. Roh HS, Lee SI, Kang YI, Kim MS, and Kim JB, "Effects of ascorbic acid, citric acid and silver nitrate on the growth of in vitro lily plantlets and reduction of browning," Journal of Plant Biotechnology 40: 224-230, 2013. http://dx.doi.org/10.5010.JPB.2013.40.4.224 https://doi.org/10.5010.JPB.2013.40.4.224