Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory and Cartilage Protection Effects of Daeganghwal-tang in MIA-induced Osteoarthritis at Rats

대강활탕(大羌活湯)이 흰쥐에서 MIA로 유발된 골관절염에 미치는 항염증 및 연골 보호 효과

  • Kim, Ju-ran (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University) ;
  • Lee, Jung Hee (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University) ;
  • Lee, Hyun-Jong (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University)
  • 김주란 (대구한의대학교 한의과대학 침구의학교실) ;
  • 이정희 (대구한의대학교 한의과대학 침구의학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구의학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구의학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구의학교실)
  • Received : 2021.08.19
  • Accepted : 2021.08.27
  • Published : 2021.08.31
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Abstract

Objectives : The purpose of this study was to evaluate the effects of Daeganghwal-tang on knee cartilage in monosodium iodoacetate(MIA)-induced osteoarthritis rats. Methods : Forty SD rats were randomly divided into five groups(n=8/group): normal group was SD rats group injected with normal saline at left knee joint and administrated orally distilled water, control group was MIA-induced osteoarthritis SD rats group administrated orally distilled water, Indomethacin group was MIA-induced osteoarthritis SD rats group administrated orally indomethacin 2 mg/kg, DGHT(L) group was MIA-induced osteoarthritis SD rats group administrated orally 1280 mg/kg of Daeganghwal-tang, and DGHT(H) group was MIA-induced osteoarthritis SD rats group administrated orally 2560 mg/kg of Daeganghwal-tang. After orally administration of drugs for 4 weeks, gross appearance and histological analysis were used to evaluate the degree of knee cartilage damage. In addition, pro-inflammatory cytokines, bone degrade factor and bone defence factors were analyzed to investigate the anti-inflammatory and cartilage protection effects of Daeganghwal-tang. Also, hematological test, biochemical test, and liver and kidney tissue were analyzed to determine the safety of Daeganghwal-tang. Results : Daeganghwal-tang inhibited the damage of the knee cartilage, and significantly prevented the reduction in cartilage thickness. In addition, the pro-inflammatory cytokines and the bone degrade factor significantly decreased, and the bone defence factors significantly increased. In the safety assessment of Daeganghwal-tang, there were no significant differences among the experimental groups and no abnormal findings were observed. Conclusions : Daeganghwal-tang has anti-inflammatory effect, inhibits cartilage damage, and protects cartilage in MIA-induced osteoarthritis rats.

Keywords

References

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