Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Comparison of Antioxidant, Wrinkle Improvement, and Whitening Efficacies of Extracts from Pinus koraiensis Cone Scale Using Extraction Methods

추출방법에 따른 잣나무(Pinus koraiensis) 구과피 추출물의 항산화, 주름개선 및 미백 효능 비교

  • Chae, Jungwoo (Gyeonggido Forest Environment Research Center) ;
  • Kim, Jeongyou (Gyeonggido Forest Environment Research Center) ;
  • Jo, Huiseon (Gyeonggido Forest Environment Research Center) ;
  • Lee, Jin-young (Division of Cosmetics and Biotechnology, Hoseo University)
  • 채정우 (경기도산림환경연구소) ;
  • 김정유 (경기도산림환경연구소) ;
  • 조희선 (경기도산림환경연구소) ;
  • 이진영 (호서대학교 화장품생명공학부)
  • Received : 2021.03.12
  • Accepted : 2021.06.25
  • Published : 2021.09.30
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Abstract

The present study evaluated the antioxidant activity, skin wrinkle reduction, and whitening activity of Pinus koraiensiscone scale extracts made with three different solvents (ethanol, supercritical fluid, and a mixture of both). Total polyphenol content was 11.03 mg/g GAE in the supercritical fluid extractand 33.79 mg/g GAE in the 70% ethanol extract. Electron donating ability of 1,000 ㎍/mL extract was 13.60% in the supercritical fluid extract, 91.37% in 70% ethanol extract, and 71.62% in mixed solvent extract. SOD-like activities in 100 ㎍/mL extract using supercritical fluid, 70% ethanol, and mixed solvents were 16.49%, 21.84%, and 10.7%, respectively. The ABTS+ radical scavenging activities of 1,000 ㎍/mL extract were 38.19%, 80.23%, and 78.72%for supercritical fluid extract, 70% ethanol extract, and mixed solvent extract, respectively. Tyrosinase inhibitory activities in 1,000 ㎍/mL extract were 24.54%, 36.55%, and 15.23% for supercritical fluid extract, 70% ethanol extract, and mixed solvent extract, respectively. Elastase inhibitory activities in 1,000 ㎍/mL extract were 15.62%, 22.56%, and 26.64% for supercritical fluid extract, 70% ethanol extract, and mixed solvent extract, respectively. Skin astringent activity (81.23% with 5000 ㎍/mL extract) was only detected in the 70% ethanol extract. Supercritical fluid and mixed solvent extracts showed no such activity. Our analysis of Pinus koraiensiscone scale extracts show that the highest aggregate activity was found in the 70% ethanol extract, followed by mixed solvent and supercritical fluid extracts. Therefore, our results oppose the hypothesis stating supercritical extract has the highest total polyphenol content and antioxidant activity.

본 연구에서 잣 구과피의 3가지 추출법(에탄올 추출, 초임계 추출, 에탄올과 초임계 추출물의 혼합)에 따른 항산화 활성, 주름개선 그리고 미백활성의 차이를 확인하였다. 그 결과, 총 폴리페놀 함량은 초임계 추출물이 11.03 mg/g GAE, 70% 에탄올 추출물이 33.79 mg/g GAE이었다. 전자공여능의 경우 1,000 ㎍/mL 농도에서 초임계 추출물은 13.60%, 70% 에탄올 추출물은 91.37%, 혼합물은 71.62%이었다. SOD 유사활성은 100 ㎍/mL 농도에서 초임계 추출물은 16.49%, 에탄올 추출물은 21.84%, 혼합물은 10.7%이었다. ABTS+ radical 소거능은 1,000 ㎍/mL 농도에서 초임계 추출물은 38.19%, 70% 에탄올 추출물은 80.23%, 혼합물은 78.72% 이었다. Tyrosinase 저해활성은 1,000 ㎍/mL 농도에서 초임계 추출물은 24.54%, 70% 에탄올 추출물은 36.55%, 혼합물은 15.23%이었다. Elastase 저해활성 측정은 1,000 ㎍/mL 농도에서 초임계 추출물은 15.62%, 70% 에탄올 추출물은 22.56%, 혼합물은 26.64%이었다. 피부수렴활성 결과는 70% 에탄올 추출물이 5000 ㎍/mL에서 81.23%이었고, 초임계 추출물과 혼합물은 활성을 나타내지 않았다. 본 연구에서 분석한 활성 결과는 잣구과피 70% 에탄올 추출물, 혼합물, 초임계 추출물 순으로 우수하게 측정되어 총 폴리페놀 함량 및 항산화 활성 모두 초임계 추출물이 높을 것으로 판단한 가설과 정반대의 결과를 나타내었다.

Keywords

Acknowledgement

본 연구는 산업단지공단 2020년도 생산기술사업화 지원사업(이전기술사업화)으로 지원받아 수행되었음(과제번호 RCC19010).

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