The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Investigation of the effect of Hibiscus sabdariffa L. extracts on tight-junction related genes in human keratinocyte HaCaT cells

히비스커스 추출물이 인간 각질 형성 세포의 밀착 연접 관련 유전자 발현에 미치는 영향 연구

  • Jung, Haesoo (Department of Biochemistry, Chungnam National University) ;
  • Kim, Eunmi (Department of Predictive Toxicology, Korea Institute of Toxicology) ;
  • Han, Hyosang (Department of Health Administration, Joongbu University) ;
  • Kim, Keekwang (Department of Biochemistry, Chungnam National University)
  • 정해수 (충남대학교 생화학과) ;
  • 김은미 (안전성평가연구소 예측독성연구본부) ;
  • 한효상 (중부대학교 보건행정학과) ;
  • 김기광 (충남대학교 생화학과)
  • Received : 2021.08.10
  • Accepted : 2021.09.25
  • Published : 2021.09.30
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Abstract

Objectives : Hibiscus (Hibiscus sabdariffa L.) is rich in antioxidants such as flavonoids and anthocyanins and is known to have anti-inflammatory activity and anti-aging function of the skin, but there is no study on its effect on the skin barrier. This study aim to investigate the positive effect on the skin barrier by confirming the effect of water extracts of Hibiscus sabdariffa L. (WEHS) on the tight junction-related gene expression. Methods : The antioxidant efficacy of WEHS was investigated through ABTS and DPPH assays, and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium was performed to examine the effect on cell viability. quantitative Reverse transcription polymerase chain reaction and immunoblot analysis were performed to confirm the effect of WEHS on the expression of tight junction-related genes, and immunofluorescence microscopy was used to confirm the movement of Claudin 1 protein into tight junctions. Results : WEHS showed strong antioxidant activity and induced an increase in both mRNA and protein expression levels of Claudin 1 among tight junction-related genes. The strong localization of Claudine 1 protein increased by WEHS to the tight junction was confirmed by immunofluorescence microscopy. Conclusions : Hibiscus was confirmed through this study to show antioxidant activity and the function of promoting the expression of the tight junction Claudin 1 gene, suggesting that Hibiscus can be used as a material for the prevention and treatment of skin moisturizing and atopy, which have an important influence on tight junction.

Keywords

Acknowledgement

본 논문은 2018년도 정부(교육부)의 재원으로 한국연구재단-이공학개인기초연구사업(기본연구지원사업)의 지원을 받아 수행된 연구임(No. 2018R1D1A1B07045689).

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