The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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First Molecular Characterization of Colletotrichum sp. and Fusarium sp. Isolated from Mangrove in Mexico and the Antagonist Effect of Trichoderma harzianum as an Effective Biocontrol Agent

  • Received : 2021.05.05
  • Accepted : 2021.08.24
  • Published : 2021.10.01
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Abstract

The aim of this study was to characterize potential fungal species affecting mangrove species in Mexico. The phytopathogens were identified based on morphological and molecular characteristics using internal transcribed spacer (ITS1/ITS4) primers then sequenced and compared with the other related sequences in GenBank (NCBI). Three fungal species were identified as Colletotrichum queenslandicum (Weir and Johnst, 2012) from black mangrove (Avicennia germinans); Colletotrichum ti (Weir and Johnst, 2012) from white mangrove (Laguncularia racemosa) and buttonwood mangrove (Conocarpus erectus); Fusarium equiseti (Corda) from red mangrove (Rhizophora mangle). In addition, C. ti and F. equiseti were identified from mango Mangifera indica L. sampled close by the mangrove area. This study provides first evidence of anthracnose on four mangrove species caused by Colletotrichum and Fusarium species in the "Términos" coastal lagoon in Campeche State southern Mexico. This is the first time that C. queenslandicum and C. ti are reported in Mexico. F. equiseti has not been reported affecting M. indica and R. mangle until the present work. Little is known regarding fungal diseases affecting mangroves in Mexico. These ecosystems are protected by Mexican laws and may be threatened by these pathogenic fungus. This is the first report of the effect of Trichoderma harzianum TRICHO-SIN as an effective biological control against of Colletotrichum and Fusarium species.

Keywords

Acknowledgement

We are thankful to the Facultad de Ciencias Naturales, Universidad Autonoma de Ciudad del Carmen (UNACAR). Also thankful to the Laboratorio de Ecofisiologia de Organismos Acuaticos de la Facultad de Ciencias del Mar, Universidad Autonoma de Sinaloa (UAS) and the Laboratorio de Inmunogenetica y Biologia Molecular de la Facultad de Quimica (UAS) for their support during this investigation.

References

  1. Adame, M. F. and Lovelock, C. E. 2011. Carbon and nutrient exchange of mangrove forests with the coastal ocean. Hydrobiologia 663:23-50. https://doi.org/10.1007/s10750-010-0554-7
  2. Ali, M. I., Yasser, M. M., Mousa, A. S. and Abdel Khalek, M. 2012. Optimization of factors affecting proliferation and flourishment of Trichoderma harzianum in Egyptian soil. J. Basic Appl. Mycol. 3:41-48.
  3. Aljanabi, S. M. and Martinez, I. 1997. Universal and rapid saltextraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 25:4692-4693. https://doi.org/10.1093/nar/25.22.4692
  4. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
  5. Bao, H., Wu, Y., Unger, D., Du, J., Herbeck, L. S. and Zhang, J. 2013. Impact of the conversion of mangroves into aquaculture ponds on the sedimentary organic matter composition in a tidal flat estuary (Hainan Island, China). Cont. Shelf Res. 57:82-91. https://doi.org/10.1016/j.csr.2012.06.016
  6. Bayen, S. 2012. Occurrence, bioavailability and toxic effects of trace metals and organic contaminants in mangrove ecosystems: a review. Environ. Int. 48:84-101. https://doi.org/10.1016/j.envint.2012.07.008
  7. Benitez, T., Rincon, A. M., Limon, M. C. and Codon, A. C. 2004. Biocontrol mechanisms of Trichoderma strains. Int. Microbiol. 7:249-260.
  8. Betancourt Resendes, I., Velazquez Monreal, J. J., Montero Castro, J. C., Fernandez Pavia, S. P., Lozoya Saldana, H. and Rodriguez Alvarado, G. 2012. Fusarium mexicanum causal agent of mango malformation in Jalisco, Mexico. Rev. Mex. Fitopatol. 30:115-127.
  9. Cai, L., Hyde, K. D., Taylor, P. W. J., Weir, B. S., Waller, J., Abang, M. M., Zhang, J. Z., Yang, Y. L., Phoulivong, S., Liu, Z. Y., Prihastuti, H., Shivas, R. G., McKenzie, E. H. C. and Johnston, P. R. 2009. A polyphasic approach for studying Colletotrichum. Fungal Divers. 39:183-204.
  10. Cannon, P. F., Damm, U., Johnston, P. R. and Weir, B. S. 2012. Colletotrichum: current status and future directions. Stud. Mycol. 73:181-213. https://doi.org/10.3114/sim0014
  11. Darriba, D., Taboada, G. L., Doallo, R. and Posada, D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9:772.
  12. Duke, N. C., Meynecke, J.-O., Dittmann, S., Ellison, A. M., Anger, K., Berger, U., Cannicci, S., Diele, K., Ewel, K. C., Field, C. D., Koedam, N., Lee, S. Y., Marchand, C., Nordhaus, I. and Dahdouh-Guebas, F. 2007. A world without mangroves? Science 317:41-42.
  13. Flores-de-Santiago, F., Kovacs, J. M. and, Flores-Verdugo, F. 2012. Seasonal changes in leaf chlorophyll a content and morphology in a sub-tropical mangrove forest of the Mexican Pacific. Mar. Ecol. Prog. Ser. 444:57-68. https://doi.org/10.3354/meps09474
  14. Food and Agriculture Organization of the United Nations. 2007. The World's Mangroves 1980-2005: A Thematic Study Prepared in the Framework of the Global Forest Resources Assessment 2005. FAO Forestry Paper 153. Food and Agriculture Organization of the United Nations, Rome, Italy. 77 pp.
  15. Guigon-Lopez, C., Guerrero-Prieto, V., Vargas-Albores, F., Carvajal-Millan, E., Avila-Quezada, G. D., Bravo-Luna, L., Ruocco, M., Lanzuise, S., Woo, S. and Lorito, M. 2010. Molecular identification of Trichoderma spp. strains, in vitro growth rate and antagonism against plant pathogen fungi. Rev. Mex. Fitopatol. 28:87-96.
  16. Gveroska, B. and Ziberoski, J. 2011. Trichoderma harzianum as a biocontrol agent against alternaria alternata on tobacco. Appl. Technol. Innov. 7:67-76. https://doi.org/10.15208/ati.2012.9
  17. Harman, G. E. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology 96:190-194. https://doi.org/10.1094/PHYTO-96-0190
  18. Hector, A. and Bagchi, R. 2007. Biodiversity and ecosystem multifunctionality. Nature 448:188-190. https://doi.org/10.1038/nature05947
  19. Hermosa, R., Rubio, M. B., Cardoza, R. E., Nicolas, C., Monte, E. and Gutierrez, S. 2013. The contribution of Trichoderma to balancing the costs of plant growth and defense. Int. Microbiol. 16:69-80.
  20. Howell, C. R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis. 87:4-10. https://doi.org/10.1094/PDIS.2003.87.1.4
  21. Isaac, M. R., Leyva-Mir, S. G., Sahagun-Gastellanos, J., CamaraCorreia, K., Tovar-Pedraza, J. M. and Rodriguez-Perez, J. E. 2018. Occurrence, identification, and pathogenicity of Fusarium spp. associated with tomato wilt in Mexico. Not. Bot. Horti. Agrobo. 46:484-493. https://doi.org/10.15835/nbha46211095
  22. Jukes, T. H. and Cantor, C. R. 1969. Evolution of protein molecules. In: Mammalian protein metabolism, ed. by H. N. Munro, pp. 21-132. Academic Press, New York, USA.
  23. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. and Drummond, A. 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647-1649. https://doi.org/10.1093/bioinformatics/bts199
  24. Kovacs, J. M., King, J. M. L., Flores de Santiago, F. and FloresVerdugo, F. 2009. Evaluating the condition of a mangrove forest of the Mexican Pacific based on an estimated leaf area index mapping approach. Environ. Monit. Assess. 157:137-149. https://doi.org/10.1007/s10661-008-0523-z
  25. Kristensen, E., Bouillon, S., Dittmar, T. and Marchand, C. 2008. Organic carbon dynamics in mangrove ecosystems: a review. Aquat. Bot. 89:201-219. https://doi.org/10.1016/j.aquabot.2007.12.005
  26. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35:1547-1549. https://doi.org/10.1093/molbev/msy096
  27. Lee, S. Y., Primavera, J. H., Dahdouh-Guebas, F., McKee, K., Bosire, J. O., Cannicci, S., Diele, K., Fromard, F., Koedam, N., Marchand, C., Mendelssohn, I., Mukherjee, N. and Record, S. 2014. Ecological role and services of tropical mangrove ecosystems: a reassessment. Glob. Ecol. Biogeogr. 23:726-743. https://doi.org/10.1111/geb.12155
  28. Liu, X., Li, B., Cai, J., Zheng, X., Feng, Y. and Huang, G. 2018. Colletotrichum species causing anthracnose of rubber trees in China. Sci. Rep. 8:10435. https://doi.org/10.1038/s41598-018-28166-7
  29. Martinez-Fernandez, E., Martinez-Jaimes, P., Sanchez, G. D., Pena-Chora, G. and Hernandez-Hernandez, V. M. 2015. Fusarium diversity from the roots of sugarcane (Saccharum officinarum) in Morelos State, Mexico. Rev. Mex. Fitopatol. 42:33-43 (in Spanish).
  30. Mora-Aguilera, A., Teliz-Ortiz, D., Mora-Aguilera, G., SanchezGarcia, P. and Javier-Mercado, J. 2003. Progreso temporal de "Escoba de bruja" (Fusarium oxysporum y F. subglutinans) en huertos de mango (Mangifera indica L.) cv. Haden en Michoacan, Mexico. Rev. Mex. Fitopatol. 21:1-12 (in Spanish).
  31. Nunes, C. A. 2012. Biological control of postharvest diseases of fruit. Eur. J. Plant Pathol. 133:181-196. https://doi.org/10.1007/s10658-011-9919-7
  32. Omar, N. H., Mohd, M., Mohamed Nor, N. M. I. and Zakaria, L. 2018. Characterization and pathogenicity of Fusarium species associated with leaf spot of mango (Mangifera indica L.). Microb. Pathog. 114:362-368. https://doi.org/10.1016/j.micpath.2017.12.026
  33. Osorio, J. A., Wingfield, M. J. and Roux, J. 2014. A review of factors associated with decline and death of mangroves, with particular reference to fungal pathogens. 2016. S. Afr. J. Bot. 103:295-301. https://doi.org/10.1016/j.sajb.2014.08.010
  34. Rojo-Baez, I., Alvarez-Rodriguez, B., Garcia-Estrada, R. S., Leon-Felix, J., Sanudo-Barajas, A. and Allende-Molar, R. 2017. Current status of Colletotrichum spp. in Mexico: taxonomy, characterization, pathogenesis and control. Rev. Mex. Fitopatol. 35:549-570.
  35. Silva-Rojas, H. V. and Avila-Quezada, G. D. 2011. Phylogenetic and morphological identification of Colletotrichum boninense: a novel causal agent of anthracnose in avocado. Plant Pathol. 60:899-908. https://doi.org/10.1111/j.1365-3059.2011.02452.x
  36. Tapia-Tussell, R., Cortes-Velazquez, A., Valencia-Yah, T., Navarro, C., Espinosa, E., Moreno, B. and Perez-Brito, D. 2016. First report of Colletotrichum magnum causing anthracnose in papaya in Mexico. Plant Dis. 100:2323.
  37. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680. https://doi.org/10.1093/nar/22.22.4673
  38. Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I. and Perez-Brito, D. 2013. Morphological, pathological and genetic diversity of Colletotrichum species responsible for anthracnose in papaya (Carica papaya L). Eur. J. Plant Pathol. 135:67-79. https://doi.org/10.1007/s10658-012-0065-7
  39. Wanderley Costa, I. P. M., Maia, L. C. and Cavalcanti, M. A. 2012. Diversity of leaf endophytic fungi in mangrove plants of northeast Brazil. Braz. J. Microbiol. 43:1165-1173. https://doi.org/10.1590/S1517-83822012000300044
  40. Weir, B. S., Johnston, P. R. and Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Stud. Mycol. 73:115-180. https://doi.org/10.3114/sim0011