한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제49권3호
  • /
  • Pages.391-402
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  • 2021
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Identification and Characterization of Microbial Community in the Coelomic Fluid of Earthworm (Aporrectodea molleri)

  • Yakkou, Lamia (Reserch Team : Lumbricidae, Improving Soil Productivity and Environment (LAPSE), Centre (Eau, Ressources Naturelles, Environnement et Developpement Durable (CERNE2D)) Mohammed V University in Rabat, Ecole Normale Superieure (ENS)) ;
  • Houida, Sofia (Reserch Team : Lumbricidae, Improving Soil Productivity and Environment (LAPSE), Centre (Eau, Ressources Naturelles, Environnement et Developpement Durable (CERNE2D)) Mohammed V University in Rabat, Ecole Normale Superieure (ENS)) ;
  • Dominguez, Jorge (Grupo de Ecoloxia Animal (GEA), Universidade de Vigo) ;
  • Raouane, Mohammed (Reserch Team : Lumbricidae, Improving Soil Productivity and Environment (LAPSE), Centre (Eau, Ressources Naturelles, Environnement et Developpement Durable (CERNE2D)) Mohammed V University in Rabat, Ecole Normale Superieure (ENS)) ;
  • Amghar, Souad (Reserch Team : Lumbricidae, Improving Soil Productivity and Environment (LAPSE), Centre (Eau, Ressources Naturelles, Environnement et Developpement Durable (CERNE2D)) Mohammed V University in Rabat, Ecole Normale Superieure (ENS)) ;
  • Harti, Abdellatif El (Reserch Team : Lumbricidae, Improving Soil Productivity and Environment (LAPSE), Centre (Eau, Ressources Naturelles, Environnement et Developpement Durable (CERNE2D)) Mohammed V University in Rabat, Ecole Normale Superieure (ENS))
  • 투고 : 2021.04.26
  • 심사 : 2021.05.18
  • 발행 : 2021.09.28
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초록

Earthworms play an important role in soil fertilization, interacting continually with microorganisms. This study aims to demonstrate the existence of beneficial microorganisms living in the earthworm's immune system, the coelomic fluid. To achieve this goal, a molecular identification technique was performed, using cytochrome c oxidase I (COI) barcoding to identify abundant endogenic earthworms inhabiting the temperate zone of Rabat, Morocco. Then, 16S rDNA and ITS sequencing techniques were adopted for bacteria and fungi, respectively. Biochemical analysis, showed the ability of bacteria to produce characteristic enzymes and utilize substrates. Qualitative screening of plant growth-promoting traits, including nitrogen fixation, phosphate and potassium solubilization, and indole acetic acid (IAA) production, was also performed. The result of mitochondrial COI barcoding allowed the identification of the earthworm species Aporrectodea molleri. Phenotypic and genotypic studies of the sixteen isolated bacteria and the two isolated fungi showed that they belong to the Pseudomonas, Aeromonas, Bacillus, Buttiauxella, Enterobacter, Pantoea, and Raoultella, and the Penicillium genera, respectively. Most of the isolated bacteria in the coelomic fluid showed the ability to produce β-glucosidase, β-glucosaminidase, Glutamyl-β-naphthylamidase, and aminopeptidase enzymes, utilizing substrates like aliphatic thiol, sorbitol, and fatty acid ester. Furthermore, three bacteria were able to fix nitrogen, solubilize phosphate and potassium, and produce IAA. This initial study demonstrated that despite the immune property of earthworms' coelomic fluid, it harbors beneficial microorganisms. Thus, the presence of resistant microorganisms in the earthworm's immune system highlights a possible selection process at the coelomic fluid level.

키워드

과제정보

The work is carried out with the support of the National Center for Scientific and Technical Research (CNRST) within the framework of the Research Excellence Scholarships Program.

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