Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Development and Validation of Dithiocarbamates Fungicide Analytical Method using CS2 Trap Method in Livestock Product

축산물 중 CS2 포집방법을 이용한 Dithiocarbamate계 살균제 분석법 개발 및 검증

  • Jo, Hyeong-Wook (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation) ;
  • Sun, Jung-Hun (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation) ;
  • Heo, Hyo-Min (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation) ;
  • Lee, Sang-Hyeob (School of Applied Biosciences, Kyongbuk National University) ;
  • Kim, Jang-Eok (School of Applied Biosciences, Kyongbuk National University) ;
  • Moon, Joon-Kwan (School of Plant Resources and Landcape Architecture, Hankyong National University)
  • 조형욱 (한경대학교 산학협력단 한살림농식품분석센터) ;
  • 선정훈 (한경대학교 산학협력단 한살림농식품분석센터) ;
  • 허효민 (한경대학교 산학협력단 한살림농식품분석센터) ;
  • 이상협 (경북대학교 응용생명과학부) ;
  • 김장억 (경북대학교 응용생명과학부) ;
  • 문준관 (한경대학교 식물자원조경학부)
  • Received : 2021.06.09
  • Accepted : 2021.06.23
  • Published : 2021.06.30
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Abstract

BACKGROUND: Dithiocarbamte fungicides have been used in crop cultivation for diseases protection and treatment. And cultivated agricultrual products were used as feedstuff, and residual pesticides are likely to be absorbed and transferred to livestock. But the maximum residue limits (MRLs) were not established for dithiocarbate fungicides in livestock products, and thus an analysis method was developed and validated for dithiocarbamate fungicides to establish MRLs. METHODS AND RESULTS: Samples were prepared using CS2 trap method and detected with UV/VIS spectrophotometer. Calibration line (0.1 ~ 10 ㎍/mL) was linear with r2 > 0.99. For validation, the recovery tests were carried out at three fortification levels (MLOQ, 10 MLOQ and 50 MLOQ) from livestock samples (egg, milk, beef, pork, and chicken). The results for mancozeb, propineb, and thiram ranged between 76.8 to 109.6%, 79.4 to 108.8%, and 80.2 to 107.8%, respectively and % RSD (relative standard deviation) values were below 9.5%. Furthermore, inter-laboratory analysis was performed to validate the method. CONCLUSION: All values were corresponded with the criteria ranges requested by both the CODEX (CAC/GL 40-1993, 2003) and MFDS guidelines (2016). This might be used as an official analytical method for determination of dithiocarbamate fungicides at established MRLs and monitoring.

Keywords

Acknowledgement

This research was supported by a grant (19162MFDS564) from Ministry of Food and Drug Safety in 2020.

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