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Sustainable water extraction of anthocyanins in aronia (Aronia melanocarpa L.) using conventional and ultrasonic-assisted method

  • Jang, Youngbin (Major of Food & Nutrition, Division of Applied Food System, Seoul Women's University) ;
  • Koh, Eunmi (Major of Food & Nutrition, Division of Applied Food System, Seoul Women's University)
  • Received : 2021.08.16
  • Accepted : 2021.09.28
  • Published : 2021.10.31

Abstract

The demand for sustainable extraction of bioactive compounds from food matrices has been increasing. Water extraction of anthocyanins in aronia was investigated using conventional and ultrasonic-assisted methods. The optimum extraction conditions for the conventional method included a sample-to-water ratio of 1:40 g/mL, extraction temperature 71℃, and extraction time of 39 min. The optimized conditions for ultrasonic-assisted extraction were a sample-to-water ratio of 1:40 g/mL, extraction temperature 80℃, extraction time of 20 min, and an amplitude of 87.2 ㎛. The anthocyanin contents of the two extracts were 155.32 and 158.02 mg/100 g fresh weight, respectively. The major anthocyanins were cyanidin 3-galactoside (65% of the total) and cyanidin 3-arabinoside (30% of the total). The contents of individual anthocyanins and phenolic acids were not significantly different between the two optimized extracts.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT). (No. 2020R1A2C1004117) This work was supported by a research grant from Seoul Women's University (2021-0372).

References

  1. Albuquerque BR, Pinela J, Barros L, Oliveria MBPP, Ferreira ICFR. Anthocyanin-rich extract of jabuticaba epicarp as a natural colorant: Optimization of heat- and ultrasound-assisted extractions and application in a bakery product. Food Chem. 316: 126364 (2020) https://doi.org/10.1016/j.foodchem.2020.126364
  2. Arancibia-Avila P, Namiesnik J, Toledo F, Werner E, Martinez-Ayala AL, Rocha-Guzman NE, Gallegos-Infante JA, Gorinstein S. The influence of different time durations of thermal processing on berries quality. Food Control. 26: 587-593 (2012) https://doi.org/10.1016/j.foodcont.2012.01.036
  3. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem. 239: 70-76 (1996) https://doi.org/10.1006/abio.1996.0292
  4. Borowska S, Brzoska MM. Chokeberries (Aronia melanocarpa) and their products as a possible means for the prevention and treatment of noncommunicable diseases and unfavorable health effects due to exposure to xenobiotics. Compr. Rev. Food Sci. Food Saf. 15: 982-1017 (2016) https://doi.org/10.1111/1541-4337.12221
  5. Boyles MJ, Wrolstad RE. Anthocyanin composition of red raspberry juice: Influences of cultivar, processing, and environmental factors. J. Food Sci. 58: 1135-1141 (1993) https://doi.org/10.1111/j.1365-2621.1993.tb06132.x
  6. Cacace JE, Mazza G. Mass transfer process during extraction of phenolic compounds from milled berries. J. Food Eng. 59: 379-389 (2003) https://doi.org/10.1016/S0260-8774(02)00497-1
  7. Cai Z, Qu Z, Lan Y, Zhao S, Ma X, Wan Q, Jing P, Li P. Conventional, ultrasound-assisted, and accelerated-solvent extractions of anthocyanins from purple sweet potatoes. Food Chem. 197: 266-272 (2016) https://doi.org/10.1016/j.foodchem.2015.10.110
  8. Chowdhury P, Viraraghavan T. Sonochemical degradation of chlorinated organic compounds, phenolic compounds and organic dyesa review. Sci. Total Environ. 407: 2474-2492 (2009) https://doi.org/10.1016/j.scitotenv.2008.12.031
  9. de Gaulejac NS, Glories Y, Vivas N. Free radical scavenging effect of anthocyanins in red wines. Food Res. Int. 32: 327-333 (1999) https://doi.org/10.1016/S0963-9969(99)00093-9
  10. Espada-Bellido E, Ferreiro-Gonzalez M, Carrera C, Palma M, Barroso CG, Barbero GF. Optimization of the ultrasound-assisted extraction of anthocyanins and total phenolic compounds in mulberry (Morus nigra) pulp. Food Chem. 219: 23-32 (2017) https://doi.org/10.1016/j.foodchem.2016.09.122
  11. Fan G, Han Y, Gu Z, Chen D. Optimizing conditions for anthocyanins extraction from purple sweet potato using response surface methodology (RSM). LWT - Food Sci. Technol. 41: 155-160 (2008) https://doi.org/10.1016/j.lwt.2007.01.019
  12. Gonzalez-de-Peredo AV, Vazquez-Espinosa M, Espada-Bellido E, Ferreiro-Gonzalez M, Carrera C, Palma M, Alvarez JA, Barbero GF, Ayuso J. Optimization of analytical ultrasound-assisted methods for the extraction of total phenolic compounds and anthocyanins from Sloes (Prunus spinosa L.). Agronomy 10: 966 (2020) https://doi.org/10.3390/agronomy10070966
  13. He B, Zheng L, Yue X, Liang J, Jiang J, Gao X, Yue P. Optimization of ultrasound-assisted extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium ashei) wine pomace. Food Chem. 204: 70-76 (2016). https://doi.org/10.1016/j.foodchem.2016.02.094
  14. Heinonen J, Farahmandazad H, Vuorinen A, Kallio H, Yang B, Sainio T. Extraction and purification of anthocyanins from purple-fleshed potato. Food Bioprod. Process. 99: 136-146 (2016) https://doi.org/10.1016/j.fbp.2016.05.004
  15. Hofmann T, Nebehja E, Stefanovits-Banyai E, Albert L. Antioxidant capacity and total phenol content of beech (Fagus sylvatica L.) bark extracts. Ind. Crop Prod. 77: 375-381 (2015) https://doi.org/10.1016/j.indcrop.2015.09.008
  16. Hwang SJ, Yoon WB, Lee O, Cha SJ, Kim JD. Radical-scavenginglinked antioxidant activities of extracts from black chokeberry and blueberry cultivated in Korea. Food Chem. 146: 71-77 (2014) https://doi.org/10.1016/j.foodchem.2013.09.035
  17. Jakobek L, Drenjancevic M, Jukic V, Seruga M. Phenolic acids, flavonols, anthocyanins and antiradical activity of "Nero", "Viking", "Galicianka" and wild chokeberries. Sci. Hortic-Amsterdam. 147: 56-63 (2012) https://doi.org/10.1016/j.scienta.2012.09.006
  18. Kong JM, Chia L, Goh N, Chia T, Brouillard R. Analysis and biological activities of anthocyanins. Phytochemistry 64: 923-933 (2003) https://doi.org/10.1016/S0031-9422(03)00438-2
  19. Ku CS, Mun SP. Optimization of the extraction of anthocyanin from Bokbunja (Rubus coreanus Miq.) marc produced during traditional wine processing and characterization of the extracts. Bioresource Technol. 99: 8325-8330 (2008) https://doi.org/10.1016/j.biortech.2008.03.013
  20. Laleh GH, Frydoonfar H, Heidary R, Jameei R, Zare S. The effect of light, temperature, pH and species on stability of anthocyanin pigments in four Berberis species. Pak. J. Nutr. 5: 90-92 (2006) https://doi.org/10.3923/pjn.2006.90.92
  21. Lee HY. Optimization of cyanidin-3-O-galactoside production from Aronia melanocarpa Elliot from nonthermal ultrasound extraction process by response surface methodology. Appl. Sci. 9: 1203 (2019) https://doi.org/10.3390/app9061203
  22. Liu X, Mu T, Sun H, Zhang M, Chen J. Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology. Food Chem. 141: 3034-3041 (2013) https://doi.org/10.1016/j.foodchem.2013.05.119
  23. Nistor M, Diaconeasa Z, Frond AD, Stirbu I, Socaciu C, Pintea A, Rugina D. Comparative efficiency of different solvents for the anthocyanins extraction from chokeberries and black carrots, to preserve their antioxidant activity. Chem. Pap. 75: 813-822 (2021) https://doi.org/10.1007/s11696-020-01344-6
  24. Oszmianski J, Lachowicz S. Effect of the production of dried fruits and juice from chokeberry (Aronia melanocarpa L.) on the content and antioxidative activity of bioactive compounds. Molecules 21: 1098 (2016) https://doi.org/10.3390/molecules21081098
  25. Patras A, Bruton NP, O'Donnell C, Tiwari BK. Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation. Trends Food Sci. Tech. 21: 3-11 (2010) https://doi.org/10.1016/j.tifs.2009.07.004
  26. Pinelo M, Sineiro J, Nunez MJ. Mass transfer during continuous solid-liquid extraction of antioxidants from grape byproducts. J. Food Eng. 77: 57-63 (2006) https://doi.org/10.1016/j.jfoodeng.2005.06.021
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  28. Rezende YRRS, Nogueira JP, Narain N. Comparison and optimization of conventional and ultrasound assisted extraction for bioactive compounds and antioxidant activity from agro-industrial acerola (Malpighia emarginata DC) residue. LWT-Food Sci. Technol. 85: 158-169 (2017) https://doi.org/10.1016/j.lwt.2017.07.020
  29. Rice-Evans CA, Miller NJ. Antioxidant activities of flavonoids as bioactive components of food. Biochem. Soc. Trans. 24: 790-795 (1996) https://doi.org/10.1042/bst0240790
  30. Rop O, Mlcek J, Jurikova T, Valsikova M, Sochor J, Reznicek V, Kramarova D. Phenolic content, antioxidant capacity, radical oxygen species scavenging and lipid peroxidation inhibiting activities of extracts of five black chokeberry (Aronia melanocarpa (Michx.) Elliot) cultivars. J. Med. Plant Res. 4: 2431-2437 (2010)
  31. Rugina D, Sconta Z, Leopold L, Pintea A, Bunea A, Socaciu C. Antioxidant activities of chokeberry extracts and the cytotoxic action of their anthocyanin fraction on HeLa human cervical tumor cells. J. Med. Food. 15: 700-706 (2012) https://doi.org/10.1089/jmf.2011.0246
  32. Santos HM, Lodeiro C, Capelo-Matinez J-L. The power of ultrasound. In: Ultrasound in chemistry: Analytical application. Capelo-Matinez J (ed). WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany (2008)
  33. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzymol. 299: 152-178 (1999) https://doi.org/10.1016/S0076-6879(99)99017-1
  34. Soria AC, Villamiel M. Effect of ultrasound on the technological properties and bioactivity of food: a review. Trends Food Sci. Tech. 21: 323-331 (2010) https://doi.org/10.1016/j.tifs.2010.04.003
  35. Sun B, Ricardo-da-Silva JM, Spranger I. Critical factors of vanillin assay for catechins and proanthocyanidins. J. Agr. Food Chem. 46: 4267-4274 (1998) https://doi.org/10.1021/jf980366j
  36. Szopa A, Kokotkiewicz A, Kubica P, Banaszczak P, WojtanowskaKrosniak A, Krosniak M, Marzec-Wroblewska U, Badura A, Zagrodzki P, Bucinski A, Luczkiewicz M, Ekiert H. Comparative analysis of different groups of phenolic compounds in fruit and leaf extracts of Aronia sp.: A. melanocarpa, A. arbutifolia, and A. ×prunifolia and their antioxidant activities. Eur. Food Res. Technol. 243: 1645-1657 (2017) https://doi.org/10.1007/s00217-017-2872-8
  37. Taheri R, Connolly BA, Brand MH, Bolling BW. Underutilized chokeberry (Aronia mealnocarpa, Aronia arbutifolia, Aronia prunifolia) accessions are rich sources of anthocyanins, flavonoids, hydroxycinnamic acids, and proanthocyanidins. J. Agr. Food Chem. 61: 8581-8588 (2013) https://doi.org/10.1021/jf402449q
  38. Tian Y, Liimatainen J, Alanne A, Lindstedt A, Liu P, Sinkkonen J, Kallio H, Yang B. Phenolic compounds extracted by acidic aqueous ethanol from berries and leaves of different berry plants. Food Chem. 220, 266-281 (2017) https://doi.org/10.1016/j.foodchem.2016.09.145
  39. Tsai P, Hsieh Y, Huang T. Effect of sugar on anthocyanin degradation and water mobility in a Roselle anthocyanin model system using 17O NMR. J. Agr. Food Chem. 52: 3097-3099 (2004) https://doi.org/10.1021/jf0306587
  40. Wang W, Ma X, Jiang P, Hu L, Zhi Z, Chen J, Ding T, Ye X, Liu D. Characterization of pectin from grapefruit peel: A comparison of ultrasound-assisted and conventional heating extraction. Food Hydrocoll. 61: 730-739 (2016) https://doi.org/10.1016/j.foodhyd.2016.06.019
  41. Wilkes K, Howard LR, Brownmiller C, Prior RL. Changes in chokeberry (Aronia melanocarpa L.) polyphenols during juice processing and storage. J. Agr. Food Chem. 62: 4018-4025 (2014) https://doi.org/10.1021/jf404281n
  42. Wong P, Yusof S, Ghazali HM, Man YBC. Optimization of hot water extraction of roselle juice using response surface methodology: a comparative study with other extraction methods. J. Sci. Food Agr. 83: 1273-1278 (2003) https://doi.org/10.1002/jsfa.1416
  43. Xu Y, Qiu Y, Ren H, Ju D, Jia H. Optimization of ultrasound-assisted aqueous two-phase system extraction of polyphenolic compounds from Aronia melanocarpa pomace by response surface methodology. Prep. Biochem. Biotechnol. 47: 312-321 (2017) https://doi.org/10.1080/10826068.2016.1244684