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찹쌀풀 첨가량에 따른 김치의 발효 중 대사산물 및 전분 구조 변화

Understanding the targeted metabolites and molecular structure of starch in Kimchi according to glutinous rice paste content during fermentation

  • 정두연 (경북대학교 식품외식산업학과) ;
  • 이주헌 (신한대학교 식품조리과학부) ;
  • 정현정 (전남대학교 식품영양과학부)
  • Jeong, Duyun (Department of Food and Food Service Industry, Kyungpook National University) ;
  • Lee, Ju Hun (Division of Food Science and Culinary Arts, Shinhan University) ;
  • Chung, Hyun-Jung (Division of Food and Nutrition, Chonnam National University)
  • 투고 : 2021.06.23
  • 심사 : 2021.07.23
  • 발행 : 2021.10.31

초록

본 연구에서는 찹쌀풀 첨가량에 따른 김치를 제조하여 저장기간에 따라 김치의 품질 특성, 당과 관련된 대사산물, 전분 구조 변화를 분석하였다. 찹쌀풀 함량이 높을수록 김치의 발효과정이 가속화하여 pH의 감소와 총균수의 증가 속도가 빠른 경향을 보여 상대적으로 빠르게 김치의 적숙기에 도달하였다. 이러한 결과는 찹쌀풀의 전분을 빠르게 가수분해하여 많은 양의 전분 유래대사산물인 glucose, maltose, mannitol을 생성하였으며, 반대로 fructose와 sorbitol 함량은 낮은 경향을 나타냈다. 김치발효 중 찹쌀풀 함량이 높을수록 아밀로펙틴 긴 사슬은 감소한 반면 짧은 사슬의 비율은 증가하였다. 결과적으로 김치 제조 시 첨가되는 찹쌀풀의 함량에 따라 전분 구조의 차이를 나타냈으며 이에 따른 대사산물과 이화학적 특성에 영향을 미치는 것을 확인하였다.

This study identified and quantified the sugar-related metabolites and analyzed the structural features of starch in kimchi added with various concentrations of glutinous rice paste during fermentation. The pH values and total bacterial counts indicated that the fermentation of kimchi with 10% glutinous rice paste progressed more quickly than that of kimchi with other rice paste concentrations. The addition of glutinous rice pastes to kimchi resulted in increased glucose and decreased fructose contents during fermentation compared to the control kimchi. Maltose content increased significantly with increasing glutinous rice paste concentrations. The average chain length distribution of starch in kimchi decreased with increasing proportions of short chains as fermentation progressed. However, the average chain length distribution of starch increased with increasing glutinous rice paste concentration in kimchi. Our results confirmed that the sugar-related metabolites and starch structural changes were highly influenced by the added starch paste concentration.

키워드

과제정보

본 논문은 2019 정부(미래창조과학부)의 재원으로 한국연구재단의 지원을 받아 수행된 중견연구사업(NRF-2019R1A2C109017111)이며 이에 감사드립니다.

참고문헌

  1. AACC. In Approved Methods of the AACC. 11th ed. Methods 76-13.01. American Association of Cereal Chemists, St. Paul, MN, USA (2000)
  2. Aarnikunnas J, Ronnholm K, Palva A. The mannitol dehydrogenase gene (mdh) from Leuconostoc mesenteroides is distinct from other known bacterial mdh genes. Appl. Environ. Microb. 59:665-671 (2002)
  3. Choi YJ, Hwang YS, Hong SW, Lee MA. Quality characteristics of kimchi according to garlic content during fermentation. J. Korean Soc. Food Sci. Nutr. 45: 1638-1648 (2016) https://doi.org/10.3746/JKFN.2016.45.11.1638
  4. Choi H, Islam MS. Antidiabetic effect of Korean traditional Baechu (Chinese cabbage) Kimchi in a type2 diabetes model of rats. J. Med. Food. 12: 292-297 (2009) https://doi.org/10.1089/jmf.2008.0181
  5. Ha JH, Hawer WS, Kim YJ, Nam YJ. Changes of free sugars in kimchi during fermentation. Korean J. Food Sci. Technol. 21: 633-683 (1989)
  6. Hahn YS, Oh JY, Song JE. The study on amylolytic enzyme and protease activities of Kimchi. Korean J. Food Sci. Technol. 34: 269-273 (2002)
  7. Hanashiro I, Abe J, Hizukuri S. A periodic distribution of the chain length of amylopectin as revealed by high-performance anion-exchange chromatography. Carbohyd. Res. 283: 151-159 (1996) https://doi.org/10.1016/0008-6215(95)00408-4
  8. Hu Y, Ketabi A, Buchko A, Ganzle MG. Metabolism of isomalto-oligosaccharides by Lactobacillus reuteri and bifidobacterial. Lett. Appl. Microbiol. 57: 108-114 (2013) https://doi.org/10.1111/lam.12076
  9. Jang MS, Park MO. Effect of glutinous rice paste on the fermentation of Puchukimchi. Korean J. Food Cook Sci. 14: 421-429 (1998)
  10. Jeong DY, Lee JH, Chung HJ. Analysis of targeted metabolites and molecular structure of starch to understand the effect of glutinous rice paste on Kimchi fermentation. Molecules. 23: 3324-3335 (2018) https://doi.org/10.3390/molecules23123324
  11. Jeong SH, Lee SH, Jung JY, Choi EJ, Jeon CO. Microbial succession and metabolite changes during long-term storage of Kimchi. J. Food Sci. 78: M763-M769 (2013)
  12. Jung JY, Lee SH, Lee HJ, Seo HY, Park WS, Jeon CO. Effects of Leuconostoc mesenteroides starter cultures on microbial communities and metabolites during Kimchi fermentation. Int. J. Food Microbiol. 153: 378-387 (2012) https://doi.org/10.1016/j.ijfoodmicro.2011.11.030
  13. Kim D, Lee S, Ro HK. Effects of fermented Dendropanax morbifera extract on the quality of Kimchi during fermentation. J. Korean Soc. Food Sci. Nutr. 48: 1120-1126 (2019) https://doi.org/10.3746/jkfn.2019.48.10.1120
  14. Lee SH, Choi WJ. Effects of medicinal herbs' extracts on the growth of lactic acid bacteria isolated from Kimchi and fermentation of Kimchi. Korean J. Food Sci. Technol. 30: 624-629 (1998)
  15. Lee GC, Han JA. Changes in the contents of total vitamin C and reducing sugars of starchy pastes added Kimchi during fermentation. Korean J. Soc. Food Sci. 14: 201-206 (1998a)
  16. Lee GC, Han JA. Changes in physical and microbial properties of starchy pastes added Kimchi during fermentation. Korean J. Soc. Food Sci. 14: 195-200 (1998b)
  17. Mheen TI, Kwon TW. Effect of temperature and salt concentration on Kimchi fermentation. Korean J. Food Sci. Technol. 16: 443-450 (1984)
  18. Park WP. The quality characteristics of Kimchi as affected by the addition of xanthan gum. J. Korean Soc. Food Sci. Nutr. 31: 423-427 (2002) https://doi.org/10.3746/JKFN.2002.31.3.423
  19. Park SE, Yoo SA, Seo SH, Lee KI, Na CS, Son HS. GC-MS based metabolomics approach of Kimchi for the understanding of Lactobacillus plantarum fermentation characteristics. LWT-Food Sci. Technol. 68: 313-321 (2016) https://doi.org/10.1016/j.lwt.2015.12.046
  20. Ryu JY, Lee HS, Rhee HS. Changes of organic acids and volatile flavor compounds in Kimchis fermented with different ingredients. Korean J. Food Sci. Technol. 16: 167-174 (1984)
  21. Seo SH, Park SE, Kim EJ, Lee KI, Na CS, Son HS. A GC-MS based metabolomics approach to determine the effect of salinity on Kimchi. Food Res. Int. 105: 492-498 (2018) https://doi.org/10.1016/j.foodres.2017.11.069
  22. Yun JY, Jeong JK, Moon SH, Park KY. Effects of brined baechu cabbage and seasoning on fermentation of kimchi. J. Korean Soc. Food Sci. Nutr. 43: 1081-1087 (2014) https://doi.org/10.3746/JKFN.2014.43.7.1081