Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Distribution and Characterization of Vibrio vulnificus Isolated in Coastal Areas of Chungcheongnam-do Province

충청남도 해안지역의 비브리오 패혈증균 분포 및 분리주의 특성 분석

  • Lee, Hyunah (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Ko, Young-Eun (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Choi, Jihye (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Lee, Dayeon (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Yeo, Seoungsoon (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Park, Jongjin (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Lee, Miyoung (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Choi, Jinha (Chungcheongnam-do Institute of Health and Environment Research) ;
  • Park, Junhyuk (Chungcheongnam-do Institute of Health and Environment Research)
  • Received : 2021.09.16
  • Accepted : 2021.10.19
  • Published : 2021.10.31
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Abstract

Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.

Keywords

Acknowledgement

이 논문은 보건환경 연구 및 시책추진 지원의 유전자 분석비용을 활용하였고, 2021년 충남도청 지식동아리 「4차 보건혁명」 회원들이 "도정 발전 및 정책 아이디어 발굴에 기여할 수 있는 과제"의 일환으로 수행되었습니다.

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