Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative detection of peri-implantitis bacteria using real-time PCR

Real-time PCR을 이용한 임플란트주위염 원인균의 정량적 분석

  • Kim, Min-Jung (Department of Dental Hygiene, Graduate School of Gachon University) ;
  • Han, Gyeong-Soon (Department of Dental Hygiene, Graduate School of Gachon University)
  • 김민정 (가천대학교 보건대학원 치위생학과) ;
  • 한경순 (가천대학교 보건대학원 치위생학과)
  • Received : 2021.08.30
  • Accepted : 2021.09.12
  • Published : 2021.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study was conducted to analyze peri-implantitis bacteria and identify their associations with health status and health activities. Methods: Gingival sulcus fluid at the implant's periodontal pockets sampled from the participants were analyzed by multiplex real time PCR. Results: Participants had strains in the order of 100% F. nucleatum, 98.0% E. corrodens, and 96.0% P. micra, and the correlation between C. rectus and E. nodatum was high (p<0.01). Diabetic group (P. gingivalis, P. nigrescens) hypertension (P. nigrescens), group with four or more periodontal pockets (P. gingivalis, T. dentica, P. intermedia, E. nodatum, and C. rectum), smoking (P. micra, E. corrodens), drinking (T. dentola), and scaling groups (C. rectus) were found to have more strains (p<0.05). Conclusions: Representative pathogenic microorganisms detected in periodontal pockets of implants were similar to dental periodontal pockets; however there were differences in the amount and distribution of microorganisms, and they were affected by health status and health behavior.

Keywords

References

  1. Flores-Guillen J, Alvarez-Novoa C, Barbieri G, Martin C, Sanz M. Five-year outcomes of a randomized clinical trial comparing bone-level implants with either submerged or transmucosal healing. J Clin Periodontol 2018;45(1):125-35. https://doi.org/10.1111/jcpe.12832
  2. Zitzmann NU, Berglundh T. Definition and prevalence of peri-implant diseases. J Clin Periodontol 2008;35(s8):286-91. https://doi.org/10.1111/j.1600-051X.2008.01274.x
  3. Salvi GE, Aglietta M, Eick S, Sculean A, Lang NP, Ramseier CA. Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans. Clin Oral Implants Res 2012;23(2):182-90. https://doi.org/10.1111/j.1600-0501.2011.02220.x
  4. Heitz Mayfield LJ. Diagnosis and management of peri-implant diseases. Aust Dent J 2008;53(51):43-8. https://doi.org/10.1111/j.1834-7819.2008.00041.x
  5. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent Jr RL. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25(2):134-44. https://doi.org/10.1111/j.1600-051x.1998.tb02419.x
  6. Stanley CH, Jeffrey LE. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the 'red complex', a prototype polybacterial pathogenic consortium in periodontitis. Periodontol 2000 2005;38(1):72-122. https://doi.org/10.1111/j.1600-0757.2005.00113.x
  7. Nguyen-Hieu T, Borghetti A, Aboudharam G. Peri-implantitis: from diagnosis to therapeutics. J Investig Clin Dent 2012;3(2):79-94. https://doi.org/10.1111/j.2041-1626.2012.00116.x
  8. Ebersole JL, Holt SC, Hansard R, Novak MJ. Microbiologic and immunologic characteristics of periodontal disease in Hispanic americans with type 2 diabetes. J Periodontol 2008;79(4):637-46. https://doi.org/10.1902/jop.2008.070455
  9. Sastrowijoto SH, Hillemans P, Van Steenbergen TJ, Abraham-Inpijn L, De Graaff J. Periodontal condition and microbiology of healthy and diseased periodontal pockets in type 1 diabetes mellitus patients. J Clin Periodontol 1989;16(5):316-22. https://doi.org/10.1111/j.1600-051x.1989.tb01662.x
  10. Moy PK, Medina D, Shetty V, Aghaloo TL. Dental implant failure rates and associated risk factors. Int J Oral Maxillofac Implants 2005;20(4):569-77.
  11. Yakob M, Soder B, Meurman JH, Jogestrand T, Nowak J, Soder PO. Prevotella nigrescens and Porphyromonas gingivalis are associated with signs of carotid atherosclerosis in subjects with and without periodontitis. J Periodontal Res 2011;46(6):749-55. https://doi.org/10.1111/j.1600-0765.2011.01398.x
  12. Lee SM, Lee HW, Lee JY. Porphyromonas gingivalis invasion of human aortic smooth muscle cells. Int J Oral Biol 2008;33(4):163-77.
  13. Hong JY. Periodontitis and systemic disease. J Kyung Hee Univ Med Cent 2017;32(1):1-8.
  14. Al-Emadi A, Bissada N, Farah C, Siegel B, Al-Zaharani M. Systemic diseases among patients with and without alveolar bone loss. Quintessence Int 2006;37(10):761-5.
  15. Lee DY, Lee MS, Kwon YH, Park JB, Herr Y, Chung JH. The influence of adjacent tooth to the microbiology of implant sulcus. J Korean Acad Periodontol 2005;35(3):563-75. https://doi.org/10.5051/jkape.2005.35.3.563
  16. Sousa V, Mardas N, Farias B, Petrie A, Needleman I, Spratt D et al. A systematic review of implant outcomes in treated periodontitis patients. Clin Oral Implants Res 2016;27(7):787-844. https://doi.org/10.1111/clr.12684
  17. Galindo-Moreno P, Fauri M, Avila-Ortiz G, Fernandez-Barbero JE, Cabrera-Leon A, Sanchez-Fernandez E. Influence of alcohol and tobacco habits on peri-implant marginal bone loss: a prospective study. Clin Oral Implants Res 2005;16(5):579-86. https://doi.org/10.1111/j.1600-0501.2005.01148.x
  18. Heitz-Mayfield LJ, Lang NP. Comparative biology of chronic and aggressive periodontitis vs. peri-implantitis. Periodontol 2000 2010;53(1):167-81. https://doi.org/10.1111/j.1600-0757.2010.00348.x
  19. Serino G, Strom C. Peri-implantitis in partially edentulous patients: association with inadequate plaque control. Clin Oral Implants Res 2009;20(2):169-74. https://doi.org/10.1111/j.1600-0501.2008.01627.x
  20. Kumar PS, Mason MR, Brooker MR, O'Brien K. Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants. J Clin Periodontol 2012;39(5):425-33. https://doi.org/10.1111/j.1600-051X.2012.01856.x
  21. Gersdorf H, Meissner A, Pelz K, Krekeler G, Gobel UB. Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. J Clin Microbiol 1993;31(4):941-6. https://doi.org/10.1128/jcm.31.4.941-946.1993
  22. Sakamoto M, Takeuchi Y, Umeda M, Ishikawa I, Benno Y. Rapid detection and quantification of five periodontopathic bacteria by real-time PCR. Microbiol Immunol 2001;45(1):39-44. https://doi.org/10.1111/j.1348-0421.2001.tb01272.x
  23. Froum SJ, Rosen PS. A proposed classification for peri-implantitis. Int J Periodontics Restorative Dent 2012;32(5):533-40.
  24. Jang GW, Kang JB. Oral health survey method 2nd ed. Seoul: Gomoonsa; 2008: 45-8, 73.
  25. Botero JE, Gonzalez, AM, Mercado RA, Olave G, Contreras A. Subgingival microbiota in peri-implant mucosa lesions and adjacent teeth in partially edentulous patients. J Periodontol 2005;76(9):1490-5. https://doi.org/10.1902/jop.2005.76.9.1490
  26. Yun JH, Park, JE, Kim DI, Lee SI, Choi SH, Cho KS et al. Identification of putative periodontal pathogens in Korean chronic periodontitis patients. J Korean Acad Periodontol 2008;38(2):143-52. https://doi.org/10.5051/jkape.2008.38.2.143
  27. Cortelli JR, Cortelli SC, Jordan S, Haraszthy VI, Zambon JJ. Prevalence of periodontal pathogens in Brazilians with aggressive or chronic periodontitis. J Clin Periodontol 2005;32(8):860-6. https://doi.org/10.1111/j.1600-051X.2005.00777.x
  28. Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol 2008;23(3):196-205. https://doi.org/10.1111/j.1399-302X.2007.00411.x
  29. Da Silva-Boghossian CM, do Souto RM, Luiz RR, Colombo AP. Association of red complex, A. actinomycetemcomitans and non-oral bacteria with periodontal diseases. Arch Oral Biol 2011;56(9):899-906. https://doi.org/10.1016/j.archoralbio.2011.02.009
  30. Nishihara T, Koseki T. Microbial etiology of periodontitis. Periodontol 2000 2004;36(1):14-26. https://doi.org/10.1111/j.1600-0757.2004.03671.x
  31. Becker W, Becker BE, Newman MG, Nyman S. Clinical and microbiologic findings that may contribute to dental implant failure. Int J Oral Maxillofac Implants 1990;5(1):31-8.
  32. Kigure T, Saito A, Seida K, Yamada S, Ishihara K, Okuda K. Distribution of Porphyromonas gingivalis and Treponema denticola in human subgingival plaque at different periodontal pocket depths examined by immunohistochemical methods. J Periodontal Res 1995;30(5):332-41. https://doi.org/10.1111/j.1600-0765.1995.tb01284.x.
  33. Sumida S, Ishihara K, Kishi M, Okuda K. Transmission of periodontal disease-associated bacteria from teeth to osseointegrated implant regions. Int J Oral Maxillofac Implants 2002;17(5):696-702.
  34. Moon KH. The influence on the recognition for periodontal care to oral micro-organism changes in dental implant patients[doctoral thesis]. Cheonan: Univ. of Dankook, 2015.
  35. Kim JH, Lee DI, Im SU, Jeon ES, Song KB, Choi YH. Oral health condition according to distribution of periodontopathic bacterial complex. J Korean Acad Oral Health 2020;44(1):41-7. https://doi.org/10.11149/jkaoh.2020.44.1.41
  36. Gmur R, Strub JR, Guggenheim B. Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. J Periodontal Res 1989;24(2):113-20. https://doi.org/10.1111/j.1600-0765.1989.tb00865.x
  37. Haffajee AD, Bogren A, Hasturk H, Feres M, Lopez NJ, Socransky SS. Subgingival microbiota of chronic periodontitis subjects from different geographic locations. J Clin Periodontol 2004;31(11):996-1002. https://doi.org/10.1111/j.1600-051X.2004.00597.x
  38. Park KH, Han GS. The effects of professional tooth cleaning and plaque control instruction on reduction of peri-implantitis. J Den Hyg Sci 2012;12(2):163-70.