한국치위생학회지 (Journal of Korean society of Dental Hygiene)

  • 제21권5호
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  • Pages.527-533
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  • 2021
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  • 2287-1705(pISSN)
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  • 2288-2294(eISSN)
한국치위생학회 (Korean Society of Dental Hygiene)
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전기분해수소수의 Streptococcus mutans에 대한 항균효과

Antibacterial effect of electrolyzed water on Streptococcus mutans

  • Kim, Ji-Hye (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Youn, Ha-Young (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Kim, Eun-Kyong (Department of Dental Hygiene, College of Science & Technology, Kyungpook National University) ;
  • Lee, Young-Eun (Department of Dental Hygiene, Daegu Health College) ;
  • Jang, Ji-Eon (Department of Dental Hygiene, Suseong University) ;
  • Song, Keun-Bae (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
  • 투고 : 2021.09.01
  • 심사 : 2021.09.20
  • 발행 : 2021.10.30
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초록

연구목적: 본 연구는 대표적인 치아우식 유발 세균인 Streptococcus mutans(S. mutans)에 대한 전기분해 수소수의 항균효과와 치면세균막 억제 효과를 평가하기 위해 실시하였다. 연구방법: BHI 액체배지 상에서 6시간 배양한 S. mutans를 전기분해수소수에 1분 또는 3분 노출시켰다. 전기분해수소수의 증식억제 효과를 확인하기 위해 균주 현탁액을 Mitis Salivarius agar bacitracin(MSB) 배지 상에서 48시간 배양한 후 집락수를 계수하였으며, biofilm formation assay에 따라 crystal violet solution을 30분간 처리하여 치면세균막을 염색하고, 600 nm의 파장에서 흡광도를 측정하여 치면세균막 형성정도를 평가하였다. 연구결과: 전기분해수소수는 S. mutans의 증식과 치면세균막 형성을 유의하게 억제하였으며(p<0.001), 특히 치면세균막 형성의 경우 노출 시간이 증가함에 따라 치면세균막 형성도 유의하게 감소하였다(p<0.05). 결론: 수돗물 전기분해수소수는 S. mutans의 증식과 치면세균막 형성 억제를 통해 치아우식을 효과적으로 예방할 수 있다고 판단된다.

Objectives: Electrolyzed water has been proven to have antibacterial effects against various microorganisms. However, there are only a few studies about effects of electrolyzed water on oral bacteria. The purpose of this study was to examine the antibacterial effect of electrolyzed water on Streptococcus mutans in vitro. Methods: S. mutans KCOM 1054 was treated with electrolyzed water for 1 or 3 minutes and plated on Mitis Salivarius agar with 15% sucrose and bacitracin. After incubation for 48 hours, colony forming units (CFU) were counted, and dental plaque was quantified by crystal violet staining. Results: The growth of S. mutans was significantly inhibited by electrolyzed water (p<0.001). In addition, the dental plaque formation by S. mutans was decreased in a time-dependent manner by exposure to electrolyzed water (p<0.001). Conclusions: Our results suggest that electrolyzed water can effectively prevent dental caries by inhibiting growth of (and the formation of dental plaque by) S. mutans.

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