Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Anti-neuroinflammatory Effects of Hwanggeumjakyak-tang on Lipopolysaccharide-induced Brain Injury Model in vivo and in vitro

지질다당류로 유발한 염증성 뇌손상 동물모델에 대한 황금작약탕의 억제효과 연구

  • Kim, Jong-gyu (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Im, Ji-sung (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • An, Sung-Hu (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, College of Korean Medicine, Wonkwang University) ;
  • Song, Yung-sun (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 김종규 (원광대학교 한의과대학 한방재활의학교실) ;
  • 임지성 (원광대학교 한의과대학 한방재활의학교실) ;
  • 안성후 (원광대학교 한의과대학 광주한방병원 한방재활의학과) ;
  • 송용선 (원광대학교 한의학전문대학원 제3의학과)
  • Received : 2021.09.06
  • Accepted : 2021.09.27
  • Published : 2021.10.31
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Abstract

Objectives Hwanggeumjakyak-tang (HJT) has traditionally been used to treat gastrointestinal inflammatory diseases; however, its protective effects against neuronal inflammation are still undiscovered. Methods We investigated the anti-neuroinflammatory effects of HJT water extract on lipopolysaccharide (LPS)-stimulated BV2 mouse microglia cells. BV2 cells were treated with LPS (1 ㎍/mL) 1 hour prior to the addition of HJT. We measured cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and nitrite production using the Griess assay. We performed a reverse transcription-polymerase chain reaction assay to measure messenger RNA expression of inflammatory cytokines including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Western blot analysis was performed to determine protein expression of mitogen-activated protein kinases (MAPKs) and inhibitor of nuclear factor kappa B (NF-κB)α. Results HJT inhibited excessive nitrite release in LPS-stimulated BV2 cells and also significantly inhibited inflammatory cytokines such as IL-1β, IL-6, and TNF-α in LPS-stimulated BV2 cells. Moreover, HJT significantly suppressed LPS-induced MAPK and NF-κB activation and inhibited the elevation of IL-1β, IL-6, and TNF-α in the brain of LPS-injected mice. Conclusions Our study highlights the anti-neuroinflammatory effects of HJT via MAPK and NF-κB deactivation.

Keywords

References

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