Journal of Ginseng Research

  • 제45권5호
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  • Pages.591-598
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  • 2021
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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The increasing hematopoietic effect of the combined treatment of Korean Red Ginseng and Colla corii asini on cyclophosphamide-induced immunosuppression in mice

  • Lee, Yuan Yee (College of Veterinary Medicine, Kyungpook National University) ;
  • Irfan, Muhammad (College of Veterinary Medicine, Kyungpook National University) ;
  • Quah, Yixian (College of Veterinary Medicine, Kyungpook National University) ;
  • Saba, Evelyn (Department of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University) ;
  • Kim, Sung-Dae (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University) ;
  • Jeong, Myung-Gyun (R&D Headquarters, Korea Ginseng Cooperation) ;
  • Kwak, Yi-Seong (R&D Headquarters, Korea Ginseng Cooperation) ;
  • Rhee, Man Hee (College of Veterinary Medicine, Kyungpook National University)
  • 투고 : 2020.11.26
  • 심사 : 2021.02.17
  • 발행 : 2021.09.30
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초록

Background: Hematopoiesis is the production of blood cells from hematopoietic stem cells (HSCs) that reside in the bone marrow. Cyclophosphamide (CTX) is a chemotherapy drug that suppresses the immune system. Korean Red Ginseng (KRG) and Colla corii asini (CCA) have been traditionally used for boosting the immune system. Methods: HSCs in the bone marrow, and immune cell subtype in splenocytes, PBMCs, and thymocytes were investigated. Serum levels of hematopoietic-related markers were analyzed using ELISA. Protein expression in spleen tissue was analyzed using western blot analysis. Hematoxylin & eosin staining in the femurs of mice were also conducted. Results: The combination of KRG and CCA with a ratio of 3:2 increased HSCs, CD3 and CD8+ T cells in the circulation, and CD3 T cells in the spleen. A ratio of 2:3 (KRG:CCA) increased the thymic regulatory T cells and recovered the CD3 T cells in the spleen and circulation while recovering proteins in the JAK-STAT pathway in the spleen. Overall, blood cell population and differentiating factors vital for cell differentiation were also significantly recovered by all combinations especially in ratios of 3:2 and 2:3. Conclusion: A ratio of 3:2 (KRG:CCA) is the most ideal combination as it recovered the HSC population in the bone marrow of mice.

키워드

과제정보

This research was supported by the Korea Ginseng Corporation.

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