Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Changes in hematological parameters and plasma components of olive flounder, Paralichthys olivaceus exposed to acute microplastics

넙치, Paralichthys olivaceus의 미세플라스틱 급성 노출에 따른 혈액성상 및 혈장성분의 변화

  • Kim, So-Hee (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • Kim, Ga-Hyun (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • Kim, Ji-Su (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • Kim, Jun-Hwan (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • Jeon, Yu-Hyeon (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Cho, Jae-Hwang (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Seok-Ryel (Department of Smart Fisheries Resources, Kongju National University) ;
  • Kim, Dae-Hee (West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 김소희 (선문대학교 수산생명의학과) ;
  • 김가현 (선문대학교 수산생명의학과) ;
  • 김지수 (선문대학교 수산생명의학과) ;
  • 김준환 (선문대학교 수산생명의학과) ;
  • 전유현 (국립수산과학원 서해수산연구소) ;
  • 조재황 (국립수산과학원 서해수산연구소) ;
  • 김석렬 (공주대학교 스마트수산자원학과) ;
  • 김대희 (국립수산과학원 서해수산연구소)
  • Received : 2021.08.06
  • Accepted : 2021.09.10
  • Published : 2021.09.30
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Abstract

Juvenile olive flounder, Paralichthys olivaceus (mean weight 66.7±7.1 g; mean length 19.2±0.9 cm) in a bio-floc environment were exposed to microplastic (PE: polyethylene, size 40-48 ㎛) at 0, 4, 20, 100, 500 and 2,500 mg L-1 for 96 hours. No P. olivaceus deaths were observed following microplastic exposure. In the plasma components, calcium was significantly decreased whereas there was no significant change with magnesium following microplastic exposure. Glucose was significantly decreased with over 100mg L-1 at 48 hours and 20mg L-1 at 96 hours. Cholesterol was significantly decreased with over 20mg L-1 after 48 hours, whereas there was no significant change in the total protein content. In enzymatic plasma components, the AST(Aspartate aminotransferase) was significant decreased by microplastic exposure. The results of this study indicate that acute exposure to microplastic induces blood physiological changes in P. olivaceus.

본 실험에서 미세플라스틱의 노출은 넙치의 혈액학적 성상인 hemoglobin 및 hematocrit 수치의 유의적인 감소를 유발하였다. 또한 미세플라스틱의 노출은 넙치 혈장 무기성분인 calcium, 유기성분인 glucose 및 cholesterol, 효소성분인 AST의 유의적인 변화를 나타냈다. 본 연구에서 바이오플락 환경에서 미세플라스틱의 노출이 어류의 혈액생리에 영향을 주며 독성으로 작용하는 것을 확인할 수 있었다. 향후, 일반 해수와 바이오플락 환경으로 각각 양성한 넙치를 이용하여 사육환경의 변화에 의한 미세플라스틱 노출영향의 차이에 대해서도 추가적인 연구가 이루어져야 할 것이다. 본 연구는 다양한 어종에서 종별 미세플라스틱의 농도 연구에서 미세플라스틱 노출에 따른 종별 독성영향비교평가를 위한 기본 자료로 활용될 수 있을 것이다. 하지만, 바이오플락 환경에서 미세플라스틱의 응집은 미세플라스틱의 독성에 영향을 미치는 중요한 요소이기 때문에(Choi et al. 2020), 향후 연구에서 철저한 모니터링과 함께 다양한 미세플라스틱 독성에 미치는 요소에 대한 추가연구가 필요할 것이다.

Keywords

Acknowledgement

이 논문은 2021년 국립수산과학원 '바이오플락을 이용한 해수양식 기술개발(대하, 넙치) (R2021014)'의 지원으로 수행된 연구입니다.

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