Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Toxic effect of chlorothalonil, an antifouling agent, on survival and population growth rate of a marine rotifer, Brachionus plicatilis

해산 로티퍼(Brachionus plicatilis)의 생존율 및 개체군 성장률을 이용한 신방오물질(Chlorothalonil)의 독성영향

  • Heo, Seung (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Lee, Ju-Wook (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Choi, Hoon (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Yoon, Sung-Jin (Ulleungdo-Docdo Ocean Science Station, Korea Institute of Ocean Science & Technology) ;
  • Kwon, Ki-Young (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Hwang, Un-Ki (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Park, Yun-Ho (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center)
  • 허승 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 이주욱 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 최훈 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 윤성진 (한국해양과학기술원 울릉도.독도 해양연구기지) ;
  • 권기영 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 황운기 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 박윤호 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터)
  • Received : 2021.05.11
  • Accepted : 2021.09.15
  • Published : 2021.09.30
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Abstract

In this study, the toxicity of chlorothalonil was investigated using survival rate and population growth rate of a marine rotifer, Brachionus plicatilis, typically used in live food in marine aquaculture systems. The survival rate of B. plicatilis was determined after 24 h of exposure to chlorothalonil (0.010-0.156 mg L-1). Population growth rate of B. plicatilis was calculated after 72 h of exposure to chlorothalonil (0.078-1.250 mg L-1). The survival rate and population growth rate of B. plicatilis exposed to chlorothalonil in single-dose toxicity assessment showed concentration-dependent reductions. Survival rates of B. plicatilis exposed to chlorothalonil had the following values: NOEC, 0.020mg L-1; LOEC, 0.039 mg L-1; and EC50, 0.057 mg L-1. Population growth rate of B. plicatilis exposed to chlorothalonil had the following values: NOEC, 0.156 mg L-1; LOEC, 0.313 mg L-1; and EC50, 0.506 mg L-1. When the residual concentration of chlorothalonil in the marine coastal area was more than 0.039 mg L-1, it had a toxic effect on B. plicatilis, a zooplankton. This paper provides toxicity values that can be used as baseline data for organizing environmental standards of chlorothalonil. It also provides insight into toxic effects of chlorothalonil on other non-target organisms.

어류 치어기의 주요 먹이로 생물로 사용되는 해산 로티퍼(Brachionus plicatilis)의 생존율 및 개체군 성장률을 이용하여, 국내 연안에 잔류하고 있는 것으로 알려진 신방오물질인 chlorothalonil의 독성을 평가하고자 하였다. B. plicatilis의 생존율은 0.039 mg L-1 에서 유의하게 감소하기 시작하여, chlorothalonil의 농도가 증가할수록 감소하는 농도의존성을 나타냈다. 생존율의 무영향농도는 0.020 mg L-1, 최소영향농도는 0.039 mg L-1, 반수영향농도는 0.057 mg L-1로 나타났다. 개체군 성장률 또한 생존율과 마찬가지로 0.313mg L-1에서부터 농도의존적으로 감소하는 경향을 나타냈다. 개체군 성장률의 무영향농도는 0.156 mg L-1, 최소영향농도는 0.313mg L-1, 반수영향농도는 0.506mg L-1로 나타났다. 본 연구 결과, 해양 생태계 내에서 신방오물질인 chlorothalonil의 잔류 농도가 0.039 mg L-1 이상일 경우 B. plicatilis에게 독성영향을 줄 것으로 예상되며, 본 연구의 생태독성 시험결과를 바탕으로 해양환경 내 chlorothalonil의 독성을 평가하기 위한 기초연구자료 및 다른 방오물질과의 독성영향을 비교평가할 수 있는 자료로 활용될 수 있을 것이다.

Keywords

Acknowledgement

본 연구는 2021년도 국립수산과학원 경상과제(R2021033) 연구비 지원으로 국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터에서 수행하였다.

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