Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Screening for resistance of Brassicaceae plant resources to clover cyst nematode

클로버씨스트선충에 대한 배추과 유전자원의 저항성 스크리닝

  • Ko, Hyoung-Rai (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Park, Eunhyeong (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Eun-Hwa (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Park, Se-Keun (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kang, Heonil (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Park, Byeongyong (Crop Protection Division, National Institute of Agricultural Sciences)
  • 고형래 (국립농업과학원 작물보호과) ;
  • 박은형 (국립농업과학원 작물보호과) ;
  • 김은화 (국립농업과학원 작물보호과) ;
  • 박세근 (국립농업과학원 작물보호과) ;
  • 강헌일 (국립농업과학원 작물보호과) ;
  • 박병용 (국립농업과학원 작물보호과)
  • Received : 2021.08.19
  • Accepted : 2021.09.06
  • Published : 2021.09.30
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Abstract

Chinese cabbage fields have been damaged by cyst nematodes in highland of Gangwon province in Korea since the year 2011, and clover cyst nematode (CCN) is one of the most problematic cyst nematodes in the crop. To investigate the plant resources for breeding new Chinese cabbage cultivar, which is resistant to CCN, screening for resistance of fifty-seven Brassicaceae plant resources to CCN was conducted. Among the plant resources, fifty-four plant resources (Brassica rapa subsp. pekinensis, B. rapa, Brassica sp., B. juncea, B. carinata, B. rapa subsp. nipposinica, B. rapa subsp. narinosa, B. rapa var. perviridis, B. rapa var. perviridis, B. napus var. napobrassica, and Eruca sativa) were very susceptible to CCN and the number of females on roots was more than 300. Two plant resources (B. carinata and B. tournefortii) with more than 100 females on roots were susceptible to CCN. However, African mustard (B. tournefortii, Korean Genebank accession no. IT218058) was resistant to CCN because of the small number of females (4±1.8) on roots. This study showed that African mustard (IT218058) was valuable as a breeding material for Chinese cabbage, which is resistant to CCN.

2017년 이후 강원도 고랭지배추는 클로버씨스트선충에 의한 피해를 받아 왔다. 저항성 배추 품종 재배는 씨스트선충 피해를 줄일 수 있는 가장 경제적인 방제 방법이다. 본 연구에서는 클로버씨스트선충 저항성 배추 신품종 육성을 위한 육종 소재 탐색을 위해 배추류 유전자원 총 57자원을 대상으로 클로버씨스트선충에 대한 저항성 검정을 수행하였다. 배추(Brassica rapa subsp. pekinensis), 순무(B. rapa), Brassica sp., 갓(B. juncea), 겨자류(B. carinata, B. tournefortii), 경수채(B. rapa subsp. nipposinica), 다채(B. rapasubsp. narinosa), 평지(B. rapa var. perviridis), 루타바가(B. napus var. napobrassica), 로켓샐러드(Eruca sativa) 54자원은 뿌리에 클로버씨스트선충 암컷이 300개 이상 증식되어 매우 감수성인 것으로 나타났다. 겨자류 2자원(B. carinata, B. tournefortii)도 클로버씨스트선충 암컷이 각각 144개, 110개 증식되어 감수성인 것으로 나타났다. 반면, 겨자류 중에서 African mustard (B. tournefortii, 씨앗은행 관리번호 IT218058)는 클로버씨스트선충 암컷이 평균 4±1.8로 증식되어 저항성인 배추류 유전자원인 것으로 나타났다. 본 연구에서 선발된 African mustard (IT218058)는 클로버씨스트선충 저항성 배추 품종 육성을 위한 육종 소재로 활용 가능할 것으로 판단된다.

Keywords

Acknowledgement

This research was supported by a grant (Project No. PJ01333202) from Rural Development Administration, Republic of Korea.

References

  1. Barker KR, CC Carter and JN Sasser. 1985. An Advanced Treatise on Meloidogyne Volume II: Methodology. North Carolina State University Graphics. Raleigh, NC.
  2. Choi YE, DG Kim and DR Choi. 1986. Selection of soybean cultivars resistant to soybean cyst nematode, Heterodera glycines. Korean J. Plant Prot. 25:53-61.
  3. Kang H, N Park, D Kim and I Choi. 2021. Screening of soybean varieties resistant to Heterodera sojae. J. Environ. Sci. Int. 30:347-352. https://doi.org/10.5322/JESI.2021.30.4.347
  4. Kim DG and YE Choi. 1983. Studies on the resistance and races of soybean-cyat nematode, Heterodera glycines, in Korea. Korean J. Plant Prot. 22:208-212.
  5. Kim DG and JK Lee. 2001. Resistance of pepper cultivars to two species of root-knot nematodes. Korean J. Appl. Entomol. 40:143-147.
  6. Kim DH, MR Cho, CY Yang, HH Kim, TJ Kang and JB Yoon. 2016. Host range screening of the sugar beet nematode, Heterodera schachtii Schmidt. Korean J. Appl. Entomol. 55:389-403. https://doi.org/10.5656/KSAE.2016.10.0.057
  7. Kim KW, HK Chung, GT Cho, KH Ma, D Chandrabalan, JG Gwag, TS Kim, EG Cho and YJ Park. 2007. PowerCore: a program applying the advanced M strategy with heuristic search for establishing core sets. Bioinformatics 23:2155-2162. https://doi.org/10.1093/bioinformatics/btm313
  8. Ko HR, SH Lee, TG Kang, TH Kim, SS Kim and JK Lee. 2020. Efficacy of soil disinfection machine for fumigants to clover cyst nematode. Res. Plant Dis. 26:72-78. https://doi.org/10.5423/RPD.2020.26.2.72
  9. Kruger DHM, JC Fourie and AP Malan. 2013. Cover crops with biofumigation properties for the suppression of plant-parasitic nematodes: a review. S. Afr. J. Enol. Vitic. 34:287-295.
  10. Kwon SB, DK Park, HS Won, YG Moon, JH Lee, YB Kim, BG Choi, HT Seo, HR Ko, JK Lee and DW Lee. 2018. Spread of cyst nematodes in highland chinese cabbage field in Gangwon-do. Korean J. Appl. Entomol. 57:339-345. https://doi.org/10.5656/KSAE.2018.11.0.037
  11. Lee JK, HR Ko and DW Lee. 2018. Efficacy of some nematicides against clover cyst nematode, Heterodera trifolii in chinese cabbage field of highland area. Korean J. Pestic. Sci. 22:69-77. https://doi.org/10.7585/kjps.2018.22.1.69
  12. Mahajan G, R Singh and BS Chauhan. 2020. Biology of Brassica tournefortii in the northern grains region of Australia. Crop Pasture Sci. 71:268-277. https://doi.org/10.1071/cp19451
  13. Mwamula AO, HR Ko, Y Kim, YH Kim, JK Lee and DW Lee. 2018. Morphological and molecular characterization of Heterodera schachtii and the newly recorded cyst nematode, H. trifolii associated with chinese cabbage in Korea. Plant Pathol. J. 34:297-307. https://doi.org/10.5423/PPJ.OA.12.2017.0262
  14. MAFRA. 2020. Agriculture, Food and Rural Affairs Statistics Yearbook. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea.
  15. Steele AE, H Toxopeus and W Heijbroek. 1983. Susceptibility of plant selections to Heterodera schachtii and a race of H. trifolii parasitic on sugarbeet in the Netherlands. J. Nematol. 15:281-288.
  16. Starr JL, J Bridge and R Cook. 2002. Resistance to plant-parasitic nematodes: history, current use and future potential. pp. 1-22. In: Plant Resistance to Parasitic Nematodes (Starr JL, R Cook and J Bridge, eds.). CABI Publishing. Wallingford, UK.
  17. Subbotin SA, M Mundo-Ocampo and J Baldwin. 2010a. Identification of Heterodera species. pp. 7-41. In: Systematics of Cyst Nematodes (Nematoda: Heteroderinae), 8A. Brill. Leiden, Netherlands.
  18. Subbotin SA, M Mundo-Ocampo and JG Baldwin. 2010b. Identification of Heterodera species. p. 512. In: Systematics of Cyst Nematodes (Nematoda: Heteroderinae), 8B. Brill. Leiden, Netherlands.
  19. Taylor AL and JN Sasser. 1978. Biology, Identification and Control of Root-Knot Nematodes. North Carolina State University Graphics. Raleigh, NC. p. 111.