Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Antioxidant and Anti-inflammatory activity of Saccharomycopsis fibuligera fermented Lindera obtusiloba flower extract

Saccharomycopsis fibuligera 발효 생강나무 꽃 추출물의 항산화 및 항염효과

  • Park, Sang-Nam (Department of Clinical Laboratory Science, KyungDong University) ;
  • Yoon, Joong-Soo (Department of Clinical Laboratory Science, KyungDong University) ;
  • Lee, Ok-Hee (Department of health management, KyungDong University)
  • 박상남 (경동대학교 임상병리학과) ;
  • 윤중수 (경동대학교 임상병리학과) ;
  • 이옥희 (경동대학교 보건관리학과)
  • Received : 2021.10.13
  • Accepted : 2021.11.20
  • Published : 2021.11.28
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Abstract

In this study, the antioxidant activity and anti-inflammatory activity of Lindera obtusiloba flower fermentation extracts were identified. To evulate antioxidant activity and antioxidant concentration, polyphenol concentration measurements, flavonoid concentrations measurements, DPPH experiments, and ABTS experiments were conducted. In Flavonoids, DPPH and ABTS assay, antioxidant activity were increased after fermentation. At this time, the flavonoid concentration was 5.0%, the DPPH experiment showed 33.27% and the ABTS experiment showed 29.82% antioxidant increase. In the anti-inflammatory experiment, we conducted a cytotoxicity experiment and an anti-inflammatory experiment for LPS-induced inflammation. Cytotoxicity showed low cytotoxicity in both control and fermentation groups, but lower cytotoxicity in fermentation groups. In the case of NO production inhibition, fermented Lindera obtusiloba flowers showed an increase in anti-inflammatory activity by more than 50% compared to the control group, showing that they can be used as a cosmetic ingredient with anti-inflammatory function.

본 연구에서는 생강나무 꽃 발효 추출물의 항산화능 및 항염능을 확인하였다. 항산화능 측정을 위해 DPPH 실험, ABTS 실험을 실시하였다. 동시에 폴리페놀 농도 측정, 플라보노이드 농도 측정을 통해 항산화 물질의 정량을 실시하였다. 플라보노이드, DPPH, ABTS에서는 발효 후의 항산화능이 증가한 것을 확인하였다. 이 때 플라보노이드 농도는 5.0%, DPPH 실험에서는 33.27%, ABTS 실험에서는 29.82%의 항산화능 개선 효과가 나타났다. 항염능 실험에서는 세포 독성 실험과 LPS로 유도된 염증에 대한 항염능을 알아보기 위한 NO 생성 억제능 실험을 실시하였다. 세포독성의 경우 대조군과 발효군 모두 낮은 세포독성을 보였지만 발효군에서 더 낮은 세포독성을 보였다. NO 생성 억제능의 경우 발효군이 대조군에 비해 50% 이상의 항염능 증가를 보여 발효 생강나무꽃이 기존 생강나무 꽃에 비해 더 개선된 화장품 원료로서 사용가능함을 보였다.

Keywords

References

  1. M. A. Jang & J. M. Lee. (2021). Research on domestic and international industrial trends of functional cosmetics. Journal of the Korean Applied Science and Technology, 38(2), 618-627. DOI : 10.12925/jkocs.2021.38.2.618
  2. Y. Gu, J. Han, C. Jiang & Y. Zhang. (2020). Biomarkers, oxidative stress and autophagy in skin aging. Ageing research reviews, 59, 101036. DOI : 10.1016/j.arr.2020.101036
  3. K. Strnadova, V. Sandera, B. Dvorankova, O. Kodet, M. Duskova, K. Smetana & L. Lacina. (2019). Skin aging: The dermal perspective. Clinics in dermatology, 37(4), 326-335. DOI : 10.1016/j.clindermatol.2019.04.005
  4. S. Zhang & E. Duan. (2018). Fighting against skin aging: the way from bench to bedside. Cell transplantation, 27(5), 729-738. DOI : 10.1177/0963689717725755
  5. T. Schikowski & A. Huls. (2020). Air pollution and skin aging. Current environmental health reports, 7(1), 58-64. DOI : 10.1007/s40572-020-00262-9
  6. L. Eckhart, E. Tschachler & F. Gruber. (2019). Autophagic control of skin aging. Frontiers in cell and developmental biology, 7, 143. DOI : 10.3389/fcell.2019.00143
  7. A. El-Kenawi & B. Ruffell. (2017). Inflammation, ROS, and mutagenesis. Cancer cell, 32(6), 727-729. DOI : 10.1016/j.ccell.2017.11.015
  8. A. Kammeyer & R. M. Luiten. (2015). Oxidation events and skin aging. Ageing research reviews, 21, 16-29. DOI : 10.1016/j.arr.2015.01.001
  9. H. Masaki. (2010). Role of antioxidants in the skin: anti-aging effects. Journal of dermatological science, 58(2), 85-90. DOI : 10.1016/j.jdermsci.2010.03.003.
  10. J. A. Evans & E. J. Johnson. (2010). The role of phytonutrients in skin health. Nutrients, 2(8), 903-928. DOI : 10.3390/nu2080903
  11. H. I. Moon & J. H. Lee. (1997). Volatile Aromatic Components of Ginger (Zingiber officinalis Roscoe) Rhizomes and Japanese Spice Bush (Lindera obtusiloba BL). Korean journal of crop science, 42(1), 7-13.
  12. H. Yun, Y. J. Lee, H. W. Seo, K. J. Park, H. N. Ko, D. S. Cha, J. Kwon, H. Jeon & K. S. Kim. (2012). Effect of Lindera obtusiloba extract on cancer metastasis. The Journal of Korean Oriental Internal Medicine, 33(4), 405-417.
  13. D. H. Won, S. B. Han, J. P. Hwang, S. J. Kim, J. Park & S. N. Park. (2012). Antioxidative Effect and Tyrosinase Inhibitory Activity of Lindera obtusiloba Blume Extracts. Journal of the society of cosmetic scientists of Korea, 38(4), 297-304. DOI : 10.15230/SCSK.2012.38.4.297
  14. H. A. Yu & C. D. Kim. (2017). Applicability of Lindera obtusiloba Flower Extracts as Cosmetic Ingredients. Asian journal of beauty and cosmetology, 15(2), 132-144. DOI : 10.20402/ajbc.2016.0088
  15. J. C. Park, Y. B. Yu, Y & J. H. Lee. (1996). Isolation and structure elucidation of flavonoid glycosides from Lindera obtusiloba BL. Journal of the Korean Society of Food and Nutrition, 25(1), 76-79
  16. R. S. Tubb. (1986). Amylolytic yeasts for commercial applications. Trends in biotechnology, 4(4), 98-104. https://doi.org/10.1016/0167-7799(86)90218-0
  17. Z. Chi, G. Liu, F. Wang, L. Ju & T. Zhang. (2009). Saccharomycopsis fibuligera and its applications in biotechnology. Biotechnology advances, 27(4), 423-431. DOI : 10.1016/j.biotechadv.2009.03.003
  18. A. Pekal & K. Pyrzynska. (2014). Evaluation of aluminium complexation reaction for flavonoid content assay. Food Analytical Methods, 7(9), 1776-1782. DOI : 10.1007/s12161-014-9814-x
  19. M. S. Blois, (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200. https://doi.org/10.1038/1811199a0
  20. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang & C. Rice-Evans. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. DOI : 10.1016/S0891-5849(98)00315-3
  21. D. H. Choi, E. H. Park & M. D. Kim. (2014). Characterization of Starch-Utilizing Yeast Saccharomycopsis fibuligera Isolated from Nuruk. Korean Journal of Microbiology and Biotechnology, 42(4), 407-412. DOI : 10.4014/kjmb.1409.09006
  22. M. Haile & W. H. Kang. (2019). Antioxidant activity, total polyphenol, flavonoid and tannin contents of fermented green coffee beans with selected yeasts. Fermentation, 5(1), 29. DOI : 10.3390/fermentation5010029
  23. C. F. Nathan & J. B. Hibbs Jr. (1991). Role of nitric oxide synthesis in macrophage antimicrobial activity. Current opinion in immunology, 3(1), 65-70. DOI : 10.1016/0952-7915(91)90079-G
  24. H. J. Jang, H. K. Lee, J. T. Bae, J. S. Lee & B. Y. Hwang. (2020). Antioxidant and Anti-i nflammatory Effects of Fermented Blackcurrant Fruit Extracts with Saccharomycopsis fibuligera. Journal of the Society of Cosmetic Scientists of Korea, 46(4), 403-413. DOI : 10.15230/SCSK.2020.46.4.403