BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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SET7-mediated TIP60 methylation is essential for DNA double-strand break repair

  • Song Hyun, Kim (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • Junyoung, Park (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • Jin Woo, Park (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • Ja Young, Hahm (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • Seobin, Yoon (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • In Jun, Hwang (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • Keun Pil, Kim (Department of Life Science, College of Natural Sciences, Chung-Ang University) ;
  • Sang-Beom, Seo (Department of Life Science, College of Natural Sciences, Chung-Ang University)
  • 투고 : 2022.05.03
  • 심사 : 2022.07.05
  • 발행 : 2022.11.30
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초록

The repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) is crucial for maintaining genomic integrity and is involved in numerous fundamental biological processes. Post-translational modifications by proteins play an important role in regulating DNA repair. Here, we report that the methyltransferase SET7 regulates HR-mediated DSB repair by methylating TIP60, a histone acetyltransferase and tumor suppressor involved in gene expression and protein stability. We show that SET7 targets TIP60 for methylation at K137, which facilitates DSB repair by promoting HR and determines cell viability against DNA damage. Interestingly, TIP60 demethylation is catalyzed by LSD1, which affects HR efficiency. Taken together, our findings reveal the importance of TIP60 methylation status by SET7 and LSD1 in the DSB repair pathway.

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과제정보

This research was supported by the National Research Foundation of Korea (NRF) grant from the Ministry of Science, ICT & Future Planning [NRF-2021R1A2C1013553] and was supported by the Chung-Ang University Research Scholarship Grants in 2020.

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