Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Qualitative Analysis of Proteins in Two Snake Venoms, Gloydius Blomhoffii and Agkistrodon Acutus

  • Ha, Su-Jeong (East West Cancer Center, Daejeon Korean Medicine Hospital, Daejeon University) ;
  • Choi, Yeo-Ok (Bio Research Institute of Biotechnology) ;
  • Kwag, Eun-Bin (East West Cancer Center, Daejeon Korean Medicine Hospital, Daejeon University) ;
  • Kim, Soo-Dam (East West Cancer Center, Daejeon Korean Medicine Hospital, Daejeon University) ;
  • Yoo, Hwa-seung (East West Cancer Center, Seoul Korean Medicine Hospital, Daejeon University) ;
  • Kang, In-Cheol (Department of Biological Science and BioChip Research Center, Hoseo University) ;
  • Park, So-Jung (East West Cancer Center, Daejeon Korean Medicine Hospital, Daejeon University)
  • Received : 2021.10.06
  • Accepted : 2022.02.08
  • Published : 2022.03.31
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Abstract

Objectives: Snake venom is a complex mixture of various pharmacologically active substances, such as small proteins, peptides, and organic and mineral components. This paper aims to identify and analyse the proteins in common venomous snakes, such as Gloydius blomhoffii (G. blomhoffii) and Agkistrodon acutus (A. acutus), in Korea. Methods: We used mass spectrometry, electrophoresis, N-terminal sequencing and in-gel digestion to analyse the proteins in these two snake venoms. Results: We identified eight proteins in G. blomhoffii venom and four proteins in A. acutus venom. The proteins detected in G. blomhoffii and A. acutus venoms were phospholipase A2, snake venom metalloproteinase and cysteine-rich secretory protein. Snake C-type lectin (snaclec) was unique to A. acutus venom. Conclusion: These data will contribute to the current knowledge of proteins present in the venoms of viper snakes and provide useful information for investigating their therapeutic potential.

Keywords

Acknowledgement

This research was supported by research grants from Daejeon University (2018).

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