Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of Antioxidant Potential and UV Protective Properties of Four Bacterial Pigments

  • Rupali, Koshti (Department of Microbiology, St. Xavier's College (Autonomous)) ;
  • Ashish, Jagtap (Department of Microbiology, St. Xavier's College (Autonomous)) ;
  • Domnic, Noronha (Department of Microbiology, St. Xavier's College (Autonomous)) ;
  • Shivali, Patkar (Department of Microbiology, St. Xavier's College (Autonomous)) ;
  • Jennifer, Nazareth (Department of Microbiology, St. Xavier's College (Autonomous)) ;
  • Ruby, Paulose (Department of Microbiology, St. Xavier's College (Autonomous)) ;
  • Avik, Chakraborty (Radiation Medicine Centre, Bhabha Atomic Research Centre) ;
  • Pampi, Chakraborty (Department of Microbiology, St. Xavier's College (Autonomous))
  • Received : 2022.05.23
  • Accepted : 2022.08.16
  • Published : 2022.09.28
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Abstract

In the present study, four distinctly colored bacterial isolates that show intense pigmentation upon brief ultraviolet (UV) light exposure are chosen. The strains are identified as Micrococcus luteus (Milky yellow), Cryseobacterium pallidum (Yellow), Cryseobacterium spp. (Golden yellow), and Kocuria turfanensis (Pink) based on their morphological and 16S rDNA analysis. Moderate salinity (1.25%), 25-37℃ temperature, and pH of 7.2 are found to be the most favorable conditions of growth and pigment production for all the selected isolates. The pigments are extracted using methanol: chloroform (1:1) and the purity of the pigments are confirmed by high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Further, Fourier transform infrared (FTIR) and UV-Visible spectroscopy indicate their resemblance with carotenoids and flexirubin family. The antioxidant activities of the pigments are estimated, and, all the pigments have shown significant antioxidant efficacy in 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The UV protective property of the pigments is determined by cling-film assay, wherein, at least 25% of UV sensitive Escherichia coli survive with bio-pigments even after 90 seconds of UV exposure compared to control. The pigments also hold a good sun protective factor (SPF) value (1.5-4.9) which is calculated with the Mansur equation. Based on these results, it can be predicted that these bacterial pigments can be further developed into a promising antioxidant and UV-protectant for several biomedical applications.

Keywords

Acknowledgement

The authors would like to thank Dr. Ankan Dutta Chowdhury, Sizouka University for his help in FTIR analysis. The authors also express their sincere gratitude to the Department of Microbiology, St. Xavier's College (Autonomous), Mumbai and Radiation Medicine Centre, BARC, Mumbai for providing laboratory facilities, and their valuable expertise for this work.

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