Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anti-Inflammatory Effects of Abalone (Haliotis discus hannai) Viscera via Inhibition of ROS Production in LPS-Stimulated RAW 264.7 Cells

  • Shin, Tai-Sun (Division of Food and Nutrition, College of Human Ecology, Chonnam National University) ;
  • Choi, Kap Seong (Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University) ;
  • Chun, Jiyeon (Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University) ;
  • Kho, Kang-Hee (Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University) ;
  • Son, Seon Ah (Division of Food and Nutrition, College of Human Ecology, Chonnam National University) ;
  • Shim, Sun-Yup (Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University)
  • Received : 2021.09.07
  • Accepted : 2021.12.11
  • Published : 2022.03.28
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Abstract

Haliotis discus hannai called abalone, is the valuable marine mollusks and the by-products of abalone processing are viscera. Brownish abalone male viscera (AMV), which have not been reported as having anti-inflammatory effects, was extracted with acetone and fractionated by different six acetone/hexane ratios (0, 10, 20, 30, 40, and 100%) using a silica column via in vitro ABTS and DPPH radical and nitric oxide (NO) production assay-guided fractionation. Among the fractions, the acetone/hexane ratio 40%, A40 exhibited the most potent radical scavenging activities and inhibition of lipopolysaccharide (LPS)-induced NO production without cytotoxicity. A40 inhibited LPS-induced intracellular reactive oxygen species (ROS) production in a dose-dependent manner. Western blot analysis revealed that A40 down-regulated the activation of NF-κB, MAPK (ERK 1/2, p-38, and JNK), and inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Moreover, this fraction inhibited the generation of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. These results suggested that AMV containing A40 with anti-inflammatory and anti-oxidantive effects, is the effective therapeutic and functional material for treating inflammatory disorders.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1A2C1013664).

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