DOI QR코드

DOI QR Code

Melanin 생성 인자 억제 효과를 통한 Mangifera indica L. Peel의 미백효과 연구

A Study on the Whitening Effect of Mangifera indica L. Peel Extracts through Inhibition of Melanin Synthesis Factor

  • 김효민 (경북대학교 약학대학) ;
  • 유단희 (서원대학교 융복합대학) ;
  • 이인철 (서원대학교 바이오코스메틱학과)
  • Kim, Hyo-Min (College of Pharmacy, Kyungpook National University) ;
  • Yoo, Dan-Hee (College of Fusion and Convergence, Seowon University) ;
  • Lee, In-Chul (Department of Bio-Cosmetic Science, Seowon University)
  • 투고 : 2021.11.01
  • 심사 : 2021.12.12
  • 발행 : 2022.03.28

초록

본 연구의 목적은 애플망고 껍질의 열수 및 70% 에탄올 추출물의 미백 효과를 검증하고자 하였다. 애플망고 껍질의 열수 및 에탄올 추출물의 미백 효과를 측정하기 위해 tyrosinase 저해 활성을 측정한 결과, 최종 농도인 1,000 ㎍/ml에서 열수 추출물은 9%, 에탄올 추출물은 35%의 저해 효과를 보였다. 세포를 통해 미백효과를 측정하고자 멜라노마세포인 B16-F10을 이용해 세포 생존율을 MTT assay를 사용하여 측정하였다. 세포 생존율 측정 결과, 100 ㎍/ml 농도에서 각각 95.64%, 103.36%의 세포 생존율을 나타내었다. 이후 실험은 세포 생존율이 95% 이상 나타난 농도인 100 ㎍/ml 이하의 농도에서 실험을 수행하였다. 미백 효과는 멜라닌 합성에 관여하는 인자의 단백질 및 mRNA 발현을 측정하여 결정하였다. 단백질 발현은 western blot을 이용하여 측정하였으며, 그 결과 MITF, tyrosinase, TRP-1 및 TRP-2에 대한 단백질 발현은 100 ㎍/ml 농도에서 열수 추출물에 의해 59%, 65%, 26%, 18% 감소하였고, 에탄올 추출물에 의해 64%, 40%, 18%, 52% 감소하였다. MITF, Tyrosinase, TRP-1 및 TRP-2의 mRNA 발현은 RT-PCR을 통해 확인하였으며, 그 결과 100 ㎍/ml에서 열수 추출물에 의해 27%, 44%, 40%, 22% 감소하였고, 에탄올 추출물에 의해 9%, 51%, 11%, 52% 감소하였다. 따라서 애플망고 껍질 추출물이 미백 효과가 있음을 확인하였고, 천연물 소재로서의 이용가치가 높을 것으로 사료되어진다.

The purpose of this study was to investigate the whitening effects of hot water (AMPW) and ethanol (AMPE) extracts of Mangifera indica L. peel. To verify the whitening effects, tyrosinase inhibitory activity was measured. 9.51% inhibitory activity, and 35.98% inhibitory activity at 1,000 ㎍/ml. The effects of AMPW and AMPE on cell viability were measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in B16-F10 melanoma cells. Greater than 95% cell viability was observed at 100 ㎍/ml. Thus, subsequent experiments were performed at concentrations less than 100 ㎍/ml. The whitening effects were confirmed by measuring the protein and mRNA expression levels of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1 (TRP-1), and TRP-2, which are factors involved in melanin synthesis. Western blotting and reverse transcription-polymerase chain reaction results confirmed that 100 ㎍/ml AMPW and AMPE showed superior inhibitory effects than the control treatment (alpha-melanocyte stimulating hormone only). Therefore, Mangifera indica L. peel extract had a whitening effect, and thus, has potential as a natural material for use in cosmetics.

키워드

참고문헌

  1. Kim DH, Park TS, Kim SG. 2019.Whitening and anti-wrinkle effect of Spirodela polyrhiza extracts. J. Appl Biol. Chem. 62: 391-398. https://doi.org/10.3839/jabc.2019.054
  2. Ha MJ. 2017. An evaluation of the physiological activities of Opuntia humi fusa fruit extract as cosmetic ingredients for acne. Konkuk University. Seoul, Korea.
  3. Lee SM. 2020. A Study of the Pharmacological Activity of Extracts from Thymus magnus and Thymus quinquecostatus on Skin. Sungshin Women's University. Seoul, Korea.
  4. Han NK, Park CM, Gwon JC, Joung MS, Choi JW. 2014. Whitening effect of Fagopyrum tataricum extract. J. Soc. Cosmet. Sci. Korea 40: 179-186. https://doi.org/10.15230/SCSK.2014.40.2.179
  5. Park TS, Kim DH, Son JH. 2015. Whitening effect of Salvia miltorrhiza bunge water extract in human epidermal melanocyte. J. Appl. Biol. Chem. 58: 333-338. https://doi.org/10.3839/jabc.2015.052
  6. Zhoh CK, Lee MH, Kim IY, Shin JY. 2016. Skin improvement effect of mask pack with snail fermented extract. J. Korean Oil Chemists' Soc. 33: 30-40. https://doi.org/10.12925/jkocs.2015.33.1.30
  7. Ann YH, Choi JS. 2006. The whitening effect by water extracts of Bombyx Mori linne. J. Korean Soc. Fashion Beauty 4: 81-86.
  8. Hida T, Kamiya T, Kawakami A, Ogino J, Sohma H, Uhara H, et al. 2020. Elucidation of melanogenesis cascade for identifying pathophysiology and therapeutic approach of pigmentary disorders and melanoma. Int. J. Mol. Sci. 21: 6129. https://doi.org/10.3390/ijms21176129
  9. Jo JB, Kim MU, Lee EH, Kim YJ, Cho EB, Kang IK, et al. 2018. Whitening effect of extracts from Matricaria chamomilla L. with B16F10 melanoma cells. J. Appl. Biol. Chem. 61: 267-273. https://doi.org/10.3839/jabc.2018.038
  10. Gillbro JM, Olsson MJ. 2011. The melanogenesis and mechanisms of skin-lightening agents - existing and new approaches. Int. J. Cosmet. Sci. 33: 210-221. https://doi.org/10.1111/j.1468-2494.2010.00616.x
  11. Hwang HS, Yoo DS, Shim JH. 2016. Whitening effect of banana leaf extract. J. Soc. Cosmet. Sci. Korea 42: 37-43. https://doi.org/10.15230/SCSK.2016.42.1.37
  12. Kim PS. 2011. Whitening effects of Angelica dahurica radix ethanol extract. J. Acad.-Ind. Technol. 12: 4038-4045.
  13. Yoo DH, Joo DH, Lee SY, Lee JY. 2015. Antioxidant effect of Nelumbo nucifera G. leaf extract and inhibition of MITF, TRP-1, TRP-2, and tyrosinase expression in a B16F10 melanoma cell line. J. Life Sci. 25: 1115-1123. https://doi.org/10.5352/JLS.2015.25.10.1115
  14. Jin KS, Oh YN, Park JA, Lee JY, Jin SJ, Hyun SK, et al. 2012. Antioxidant, anti-melanogenic, and anti-inflammatory activities of Zanthoxylum schinifolium extract and its solvent fractions. Korean J. Microbiol. Biotechnol. 40: 371-379. https://doi.org/10.4014/kjmb.1207.07023
  15. Parvez S, Malik KA, Ah Kang S, Kim HY. 2006. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100: 1171-1185. https://doi.org/10.1111/j.1365-2672.2006.02963.x
  16. Jung DH, Shin JH, Cho YY, Son JE. 2015. Development of a two-variable spatial leaf photosynthetic model of irwin mango grown in greenhouse. Protected Horticulture Plant Factory 24: 161-166. https://doi.org/10.12791/KSBEC.2015.24.3.161
  17. An MR, Keum YS, Lee SK. 2015. Comparative analysis of volatile flavor compounds in Taiwan apple mango and Philippines carabao mango. Korean J. Food Sci. Technol. 47: 191-197. https://doi.org/10.9721/KJFST.2015.47.2.191
  18. Naef R, Velluz A, Jaquier A. 2006. The perfume of carabao mangoes (Mangifera indica L.): Identification of uncommon unsaturated fatty acid esters in the SPME of the intact fruit. Eur. Food Res. Technol. 222: 554-558. https://doi.org/10.1007/s00217-005-0132-9
  19. Ji ST, Youm JW, Yoo JY. 2018. A feasibility study on the cultivation of tropical fruit in Korea: Focused on nango. J. Korean Acad.-Ind. Cooperation Soc. 19: 252-263.
  20. Han SH, Kim JS, Teruya T, Teruya Y, Moromizato I, Lim CK. 2016. Comparison of the fruit qualities, the free radical scavenging activities and mangiferin content of the mango, cv. irwin cultivated in Jeju and Okinawa. Korean J. Hortic. Sci. Technol. 34: 634-643.
  21. Jung ES. 2007. Antioxidant and antibacterial activity of extracts from Mangifera indica. Sunchon National University. Sunchun, Korea.
  22. Sivakumar D, Jiang Y, Yahia EM. 2011. Maintaining mango (Mangifera indica L.) fruit quality during the export chain. Food Res. Int. 44: 1254-1263. https://doi.org/10.1016/j.foodres.2010.11.022
  23. Yagi A, Kanbara T, Morinobu N. 1987. Inhibition of mushroom-tyrosinase by aloe extract. Planta Med. 53: 515-517. https://doi.org/10.1055/s-2006-962798
  24. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
  25. Kwak JH, Seo UK, Han YH. 2001. Inhibitory effect of Mugwork extracts on tyrosinase activity. Korean J. Biotechnol. Bioeng. 16: 220-223.
  26. Song HY, Sung NY, Jung PM, Kang MS, Park WJ, Byun EH. 2015. Whitening effect of green tea seed shell ethanol extracts. J. Korean Soc. Food Sci. Nutr. 44: 1470-1475. https://doi.org/10.3746/JKFN.2015.44.10.1470
  27. Kim YE, Yang JW, Lee CH, Kwon EK. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake sing. (Pine Mushroom). J. Korean Soc. Food Sci. Nutr. 38: 555-560. https://doi.org/10.3746/JKFN.2009.38.5.555
  28. Del Marmol V, Beermann F. 1996. Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381: 165-168. https://doi.org/10.1016/0014-5793(96)00109-3
  29. Kim DS. 2003. Sphingosine-1-phosphate decreases melanin synthesis via sustained ERK activation and subsequent MITF degradation. J. Cell Sci. 116: 1699-1706. https://doi.org/10.1242/jcs.00366
  30. Joo DH, Yoo DH, Lee JY. 2019. A study on the whitening effect of Erigeron annuus (L.) Pers. ethanol extract on melanoma cell (B16F10). Microbiol. Biotechnol. Lett. 47: 148-157. https://doi.org/10.4014/mbl.1803.03017
  31. Kim IH, Lee JH. 2018. Antibacterial and whitening activities of Coffea arabica ethanol extract. Korean Chem. Eng. Res. 56: 245-251.