Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240

  • Le, Huong Giang (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kim, Min Jae (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Jeon, Hye Sung (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Yoo, Ji Yeon (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kang, Yun Ji (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kim, Tae Jin (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University) ;
  • Kim, Jeong Hwan (Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University)
  • Received : 2022.01.12
  • Accepted : 2022.02.22
  • Published : 2022.03.28
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Abstract

Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria-Escherichia coli shuttle vectors.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1A2C1008267). Le HG, Kim MJ, and Kim TJ were supported by full-time graduate student scholarship from Gyeongsang National University. Jeon HS, Yoo JY, and Kang YJ were supported by BK21 Four program from MOE, Korea.

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