Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-Obesity Effect of Pinus densiflora Leaf Extracts

솔잎(Pinus densiflora leaf) 추출물의 항비만효과

  • Choi, Min Yeong (Department of Medicinal Plant Resources, Andong National University) ;
  • Shin, Byel (PINOGEN Co., Ltd) ;
  • Yu, Ju Hyeong (Department of Medicinal Plant Resources, Andong National University) ;
  • Yeo, Joo Ho (Department of Medicinal Plant Resources, Andong National University) ;
  • Lee, Jae Won (Department of Medicinal Plant Resources, Andong National University) ;
  • Geum, Na Gyeong (Department of Medicinal Plant Resources, Andong National University) ;
  • An, Mi-Yun (National Forest Seed and Variety Center) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
  • 최민영 (국립안동대학교 생약자원학과) ;
  • 신별 (농업회사법인 (주)피노젠) ;
  • 유주형 (국립안동대학교 생약자원학과) ;
  • 여주호 (국립안동대학교 생약자원학과) ;
  • 이재원 (국립안동대학교 생약자원학과) ;
  • 금나경 (국립안동대학교 생약자원학과) ;
  • 안미연 (국립산림품종관리센터) ;
  • 정진부 (국립안동대학교 생약자원학과)
  • Received : 2022.02.11
  • Accepted : 2022.03.08
  • Published : 2022.04.01
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Abstract

In this study, we measured the inhibitory activity of Pinus densiflora leaf (PDL) against excessive lipid accumulation in mouse preadipocyte, 3T3-L1 cells to investigate whether PDL exerts anti-obesity activity. Lipid accumulation and the protein level were measured using Oil red O staining assay and Western blot analysis, respectively. We observed that PDL inhibited excessive lipid accumulation and decreased the expression of CEBPα, PPARγ and perilipin-1 related to lipid accumulation in 3T3-L1 cells. Therefore, considering these results, PDL can be used as a potential agent for anti-obesity.

본 연구에서는 솔잎 추출물(PDL)의 항비만활성을 검증하기 위해, PDL이 3T3-L1에서 DMI/insulin에 의해 유도되는 과도한 지질 형성을 억제하는지 평가하였다. 본 결과를 미루어 볼 때, PDL은 지방세포의 adipogenesis과 관련된 PPARγ, CEBPα 그리고 perilipin-1의 발현을 억제하여 과도한 지질형성을 억제하는 것으로 판단되며, PDL은 향후 비만 예방 및 치료를 위한 잠재적 소재로 활용이 가능할 것으로 판단된다. 그러나 본 연구는 PDL이 항비만 활성이 있는지 검증한 초기 단계 연구로 추가적인 기전연구와 동물기반 전임상 항비만활성 검증이 필요하다.

Keywords

Acknowledgement

본 연구는 한국연구재단 이공분야 중점연구소지원사업(NRF-2018R1A6A1A03024862)의 지원에 의해 이루어진 결과로 이에 감사드립니다.

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