Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Identification of 19 Species of Poisonous Plants from Jeju Island and Construction of a Database Using DNA-barcoding

DNA-barcoding을 이용한 제주도 자생 독성 식물 19종의 종 식별 및 데이터베이스 구축

  • Kwon, Eunchae (Forensic DNA Division, National Forensic Service) ;
  • Kim, Joo-Young (Forensic DNA Division, National Forensic Service) ;
  • Chang, Miwha (Forensic DNA Division, National Forensic Service) ;
  • Lee, Minji (Forensic DNA Division, National Forensic Service) ;
  • Moon, Seohyun (Forensic DNA Division, National Forensic Service) ;
  • Lee, Won-Hae (Forensic DNA Division, National Forensic Service)
  • 권은채 (국립과학수사연구원 유전자과) ;
  • 김주영 (국립과학수사연구원 유전자과) ;
  • 장미화 (국립과학수사연구원 유전자과) ;
  • 이민지 (국립과학수사연구원 유전자과) ;
  • 문서현 (국립과학수사연구원 유전자과) ;
  • 이원해 (국립과학수사연구원 유전자과)
  • Received : 2021.12.09
  • Accepted : 2022.03.25
  • Published : 2022.04.01
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Abstract

Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.

독성 식물로 인한 식중독 사고는 매년 발생하고 있으며, 일부 독성 식물은 식용 식물로 오인 섭취되어 식중독을 유발하기 때문에 독성 식물의 정확한 종 식별이 요구되고 있다. 이러한 요구에 따라 감정기관에서는 독성 식물의 종 식별에 적합한 DNA 바코드를 찾아 신속하고 정확한 종 식별에 활용할 수 있는 데이터베이스를 구축하는 것이 필요한 실정이다. 따라서 본 연구는 다양한 독성 식물에서 DNA 바코드 구간의 염기서열을 확보하고, 각각에 적합한 DNA 바코드를 확인하여 데이터베이스를 구축하고자 하였으며, 기초 연구로써 제주도에 자생하는 독성식물 19종을 선정하여 7개의 DNA 바코드 (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA)를 이용한 종식별을 수행하였다. 종 식별 결과 trnL-trnF 바코드와 ITS1-ITS4 바코드가 PCR 증폭 및 염기서열 획득에 가장 용이하였으며, 두 개의 바코드를 조합하여 사용하면 19종 중 18종의 식물에서 단일 종 식별이 가능하였다. 따라서 미지의 독성 식물에 대한 감정이 의뢰되었을 때 trnL-trnF 바코드와 ITS1-ITS4 바코드를 조합하여 사용하면 신속한 종 식별에 도움이 될 것으로 사료된다. 본 연구에서 제시된 독성식물 19종의 염기서열 및 DNA 바코드 데이터베이스는 더욱 신속하고 정확한 독성 식물의 종 식별 감정에 도움이 될 것이다.

Keywords

Acknowledgement

이 논문은 행정안전부 주관 국립과학수사연구원 중장기과학 수사감정기법연구개발(R&D)사업의 지원을 받아 수행한 연구임(NFS2022DNA01).

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