DOI QR코드

DOI QR Code

Physicochemical, Antibacterial Properties, and Compatibility of ZnO-NP/Chitosan/β-Glycerophosphate Composite Hydrogels

  • Huang, Pingping (The Affiliated Hospital of Qingdao University) ;
  • Su, Wen (School of Stomatology of Qingdao University) ;
  • Han, Rui (The Affiliated Hospital of Qingdao University) ;
  • Lin, Hao (School of Stomatology of Qingdao University) ;
  • Yang, Jing (School of Stomatology of Qingdao University) ;
  • Xu, Libin (School of Stomatology of Qingdao University) ;
  • Ma, Lei (The Affiliated Hospital of Qingdao University)
  • 투고 : 2021.11.12
  • 심사 : 2022.01.05
  • 발행 : 2022.04.28

초록

In this study we aimed to develop novel ZnO-NP/chitosan/β-glycerophosphate (ZnO-NP/CS/β-GP) antibacterial hydrogels for biomedical applications. According to the mass fraction ratio of ZnO-NPs to chitosan, mixtures of 1, 3, and 5% ZnO-NPs/CS/β-GP were prepared. Using the test-tube inversion method, scanning electron microscopy and Fourier-transform infrared spectroscopy, the influence of ZnO-NPs on gelation time, chemical composition, and cross-sectional microstructures were evaluated. Adding ZnO-NPs significantly improved the hydrogel's antibacterial activity as determined by bacteriostatic zone and colony counting. The hydrogel's bacteriostatic mechanism was investigated using live/dead fluorescent staining and scanning electron microscopy. In addition, crystal violet staining and MTT assay demonstrated that ZnO-NPs/CS/β-GP exhibited good antibacterial activity in inhibiting the formation of biofilms and eradicating existing biofilms. CCK-8 and live/dead cell staining methods revealed that the cell viability of gingival fibroblasts (L929) cocultured with hydrogel in each group was above 90% after 24, 48, and 72 h. These results suggest that ZnO-NPs improve the temperature sensitivity and bacteriostatic performance of chitosan/β-glycerophosphate (CS/β-GP), which could be injected into the periodontal pocket in solution form and quickly transformed into hydrogel adhesion on the gingiva, allowing for a straightforward and convenient procedure. In conclusion, ZnO-NP/CS/β-GP thermosensitive hydrogels could be expected to be utilized as adjuvant drugs for clinical prevention and treatment of peri-implant inflammation.

키워드

과제정보

This work was supported by a grant from the Qingdao Livelihood Science and Technology Project (Project No. 19-6-1-33-nsh).

참고문헌

  1. Derks J, Tomasi C. 2015. Peri-implant health and disease. A systematic review of current epidemiology. J. Clin. Periodontol. 42: S158. https://doi.org/10.1111/jcpe.12334
  2. French D, Ofec R, Levin L. 2021. Long term clinical performance of 10871 dental implants with up to 22 years of follow-up: a cohort study in 4247 patients. Clin. Implant Dent. Relat. Res. 23: 289-297. https://doi.org/10.1111/cid.12994
  3. Ryan L Wong, Sarah Hiyari, Aline Yaghsezian, Mina Davar, Yi-Ling Lin, Maria Galvan, et al. 2017. Comparing the healing potential of late-stage periodontitis and peri-implantitis. J. Oral Implantol. 43: 437-445. https://doi.org/10.1563/aaid-joi-D-17-00157
  4. Birang E, Birang R, Narimani T, Alaleh Tolouei, Reza Fekrazad . 2019. Investigation of the antibacterial effect of laser irradiation and chemical agent on human oral biofilms contaminated titanium discs. Photodiagnosis Photodyn. Ther. 25: 259-264. https://doi.org/10.1016/j.pdpdt.2018.12.008
  5. Kato A, Imai K, Ochiai K, Ogata Y. 2015. Prevalence and quantitative analysis of Epstein-Barr virus DNA and Porphyromonas gingivalis associated with Japanese chronic periodontitis patients. Clin. Oral Investig. 19: 1605-1610. https://doi.org/10.1007/s00784-014-1387-y
  6. Lasserre JF, Brecx MC, Toma S. 2018. Oral microbes, biofilms and their role in periodontal and peri-implant diseases. Materials 11: 1802. https://doi.org/10.3390/ma11101802
  7. Koldsland OC, Aass AM. 2020. Supportive treatment following peri-implantitis surgery: An RCT using titanium curettes or chitosan brushes. J. Clin. Periodontol. 47: 1259-1267. https://doi.org/10.1111/jcpe.13357
  8. Zhang T, Kalimuthu S, Rajasekar V. 2021. Biofilm inhibition in oral pathogens by nanodiamonds. Biomater. Sci. 9: 5127-5135. https://doi.org/10.1039/D1BM00608H
  9. Saquib SA, AlQahtani NA, Ahmad I. 2019. Evaluation and comparison of antibacterial efficacy of herbal extracts in combination with antibiotics on periodontal pathobionts: an in vitro microbiological study. Antibiotics (Basel) 8: 89. https://doi.org/10.3390/antibiotics8030089
  10. Eom SH, Kang SK, Lee DS, Myeong JI. 2016. Synergistic antibacterial effect and antibacterial action mode of chitosan-ferulic acid conjugate against methicillin-resistant Staphylococcus aureus. J. Microbiol. Biotechnol. 26: 784-789. https://doi.org/10.4014/jmb.1511.11046
  11. Yan W, Zhang Q, Zhang CL. 2012. Characterisation and cooperative antimicrobial properties of chitosan/nano-ZnO composite nanofibrous membranes. Food Chem. 132: 419-427. https://doi.org/10.1016/j.foodchem.2011.11.015
  12. Xinhui Zhang, Balarabe B Ismail, Huan Cheng, Tony Z Jin, Mengyan Qian, Saifanassour Ali Arabi, et al. 2021. Emerging chitosanessential oil films and coatings for food preservation- A review of advances and applications. Carbohydr. Polym. 273: 118616. https://doi.org/10.1016/j.carbpol.2021.118616
  13. S V Otari, S H Pawar, Sanjay K S Patel, Raushan K Singh, Sang-Yong Kim, Jai Hyo Lee, et al. 2017. Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles: characterization, antimicrobial activity, and toxicity studies. J. Microbiol. Biotechnol. 27: 731-738. https://doi.org/10.4014/jmb.1610.10019
  14. Fonseca-Garcia A, Caicedo C, Jimenez-Regalado EJ. 2021.Effects of poloxamer content and storage time of biodegradable Starch-Chitosan films on its thermal, structural, mechanical, and morphological properties. Polymers (Basel) 13: 2341. https://doi.org/10.3390/polym13142341
  15. Hoomaan Joz Majidi, Amir Babaei, Zahra Arab Bafrani, Dina Shahrampour, Erfan Zabihi, Seid Mahdi Jafari. 2019. Investigating the best strategy to diminish the toxicity and enhance the antibacterial activity of graphene oxide by chitosan addition. Carbohydr. Polym. 225: 115220-. https://doi.org/10.1016/j.carbpol.2019.115220
  16. Wang Wenjun, Qin Peng, Shi Peiru, Wu Ziman, Wu Pinyun, Yu Lin. 2021. Antibacterial effect of chitosan-modified Fe3O4 nanozymes on Acinetobacter baumannii. J. Microbiol. Biotechnol. 32: 263-267.
  17. Isadora Martini Garcia , AbdulRahman A Balhaddad, Maria S Ibrahim, Michael D Weir, Hockin H K Xu, Fabricio Mezzomo Collares, et al. 2020. Antibacterial response of oral microcosm biofilm to nano-zinc oxide in adhesive resin. Dent. Mater. 37: e182-e193.
  18. Xiyue Zhang , Zheng Zhang , Wenyi Wu, Jun Yang, Qing Yang. 2021. Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity. Bioprocess Biosyst. Eng. 44: 1193-1199. https://doi.org/10.1007/s00449-021-02521-x
  19. Riaz A, Lagnika C, Abdin M, Hashim M. 2020. Preparation and characterization of chitosan/gelatin-based active food packaging films containing apple peel nanoparticles. J. Polym. Environ. 28: 411-420. https://doi.org/10.1007/s10924-019-01619-4
  20. Wasupalli GK, Verma D. 2020. Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering. Mater. Sci. Eng. C, Mater. Biol. Appl. 107: 110343. https://doi.org/10.1016/j.msec.2019.110343
  21. Kalita S, Kandimalla R, Sharma KK, Kataki AC. 2016. Amoxicillin functionalized gold nanoparticles reverts MRSA resistance. Mater. Sci. Eng. C, Mater. Biol. Appl. 61: 720-727. https://doi.org/10.1016/j.msec.2015.12.078
  22. Wang L, Li C, Weir MD, Zhang K. 2017. Novel multifunctional dental bonding agent for Class-V restorations to inhibit periodontal biofilms. RSC Adv. 46: 29004-29014. https://doi.org/10.1039/C6RA28711E
  23. Victor R , Santos A , Sousa B. 2020. A review on chitosan's uses as biomaterial: tissue engineering, drug delivery systems and cancer treatment. Materials (Basel). 13: 4995. https://doi.org/10.3390/ma13214995
  24. Choudhury AJ, Gogoi D, Kandimalla R, Kalita S. 2016. Penicillin impregnation on oxygen plasma surface functionalized chitosan/Antheraea assama silk fibroin: Studies of antibacterial activity and antithrombogenic property. Mater. Sci. Eng. C, Mater. Biol. Appl. 60: 475-484. https://doi.org/10.1016/j.msec.2015.11.070
  25. Apatzidou D, Lappin DF, Hamilton G , Riggio MP. 2017. Microbiome of peri-implantitis affected and healthy dental sites in patients with a history of chronic periodontitis. Arch Oral. Biol. 83: 145-152. https://doi.org/10.1016/j.archoralbio.2017.07.007
  26. Javed R, Rais F, Fatima H et al. 2020. Chitosan encapsulated ZnO nanocomposites: fabrication, characterization, and functionalization of bio-dental approaches. Mater. Sci. Eng. C, Mater. Biol. Appl. 116: 111184. https://doi.org/10.1016/j.msec.2020.111184
  27. Zheng Y, Wang W, Zhao J. 2019. Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer. Carbohydr. Polym. 222: 115039. https://doi.org/10.1016/j.carbpol.2019.115039
  28. Xu HH, Simon CG Jr. 2005. Fast setting calcium phosphate-chitosan scaffold: mechanical properties and biocompatibility. Biomaterials 26: 1337-1348. https://doi.org/10.1016/j.biomaterials.2004.04.043
  29. Wang B, Zhang Y, Mao Z. 2014.Toxicity of ZnO nanoparticles to macrophages due to cell uptake and intracellular release of zinc ions. J. Nanosci. Nanotechnol. 14: 5688-5696. https://doi.org/10.1166/jnn.2014.8876
  30. Jayasuriya AC, Aryaei A, Jayatissa AH. 2013. ZnO nanoparticles induced effects on nanomechanical behavior and cell viability of chitosan films. Mater. Sci. Eng. C, Mater. Biol. Appl. 33: 3688-3696. https://doi.org/10.1016/j.msec.2013.04.057
  31. Li Z, Yubao L, Yi Z, Lan W. 2010. In vitro and in vivo evaluation on the bioactivity of ZnO containing nano-hydroxyapatite/chitosan cement. J. Biomed. Mater. Res. A. 93: 269-79.
  32. Careta O, Fornell J, Pellicer E. 2021. ZnO Nanosheet-coated TiZrPdSiNb alloy as a piezoelectric hybrid material for self-stimulating orthopedic implants. Biomedicines 9: 352. https://doi.org/10.3390/biomedicines9040352
  33. Xiyue Zhang, Zheng Zhang , Wenyi Wu , Jun Yang, Qing Yang. 2021.Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity. Bioprocess Biosyst. Eng. 44: 1193-1199. https://doi.org/10.1007/s00449-021-02521-x
  34. Kassem AA, Ismail FA, Naggar VF. 2014.Comparative study to investigate the effect of meloxicam or minocycline HCl in situ gel system on local treatment of periodontal pockets. AAPS PharmSciTech 15: 1021-1028. https://doi.org/10.1208/s12249-014-0118-7
  35. Belibasakis GN, Manoil D. 2021. Microbial community-driven etiopathogenesis of peri-implantitis. J. Dent. Res. 100: 21-28. https://doi.org/10.1177/0022034520949851