Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Influence of β-carotene enhanced transgenic soybean cultivation on the diversity of non-target arthropods in Korea for three years

  • Sung-Dug Oh (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Eunji Bae (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Soo-Yun Park (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong-Kon Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Doh-Won Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kihun Ha (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Minwook Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Yeongjin Son (School of Applied Biosciences, Kyungpook National University) ;
  • Chang Uk Eun (School of Applied Biosciences, Kyungpook National University) ;
  • Young-Kun Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Junho Lee (School of Applied Biosciences, Kyungpook National University) ;
  • Dongmin Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Donguk Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Jongwon Kim (Division of Zoology, Honam National Institute of Biological Resources) ;
  • Sang Jae Suh (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.08.22
  • Accepted : 2022.09.20
  • Published : 2022.12.01
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Abstract

Environmental risk assessment of living modified (LM) crops is essential for their cultivation. In this study, we cultivated β-carotene enhanced transgenic soybean (LM soybean) and non-LM soybean (Gwangan) in living modified organism (LMO) isolated fields, and investigated changes in the insect fauna using three types of collection methods for three years. In total, 331,483 individual insects and arachnids, representing 82 families in 14 orders, were captured during the study. Totals of 166,518 and 164,965 individual insects and arachnids were collected from LM soybean and Gwangan, respectively. Throughout the study, although there were differences between the investigation year, region, and methods, there were no significant differences between the population densities of insect pests, natural enemies, and other insects on LM soybean and non-LM soybean. Also, there were no statistically significant differences between varieties in the results of the species diversity analysis. The data on insect species population densities were subjected to multidimensional scaling (MDS), which did not distinguish between the two varieties, LM soybean and the non-LM soybean, in all cultivated fields. However, the results of the MDS analysis were completely divided into six groups based on the yearly survey areas. These results provided the insect diversity for an environmental risk assessment of LM soybean and suggested that the guideline could be useful to detect LM crops.

Keywords

Acknowledgement

본 연구는 농촌진흥청 농촌진흥청 연구개발사업(과제번호: PJ0157502022, PJ01368001)의 지원으로 수행되었습니다.

References

  1. Aijie LSY, Li L. 2014. Structure, trypsin inhibitor activity and functional properties of germinated soybean protein isolate. International Journal of Food Science & Technology 49:911-919.  https://doi.org/10.1111/ijfs.12386
  2. Amin MR, Oh SD, Bae EJ, Park SY, Suh SJ. 2020a. Impact of insect-resistant transgenic rice on above-ground non-target arthropods in Korea. Entomological Research 50:525-538.  https://doi.org/10.1111/1748-5967.12473
  3. Amin MR, Oh SD, Suh SJ. 2020b. Comparing the effects of GM and non-GM soybean varieties on non-target arthropods. Entomological Research 50:423-432.  https://doi.org/10.1111/1748-5967.12461
  4. Bendich A, Olson JA. 1989. Biological actions of carotenoids. FASEB Journal 3:1927-1932.  https://doi.org/10.1096/fasebj.3.8.2656356
  5. Borror DJ, Triplehorn CA, Johnson NF. 1989. An introduction to the study of insects, 6th ed. Saunders College Publishing, Florida, USA. 
  6. Cho JI, Park SH, Lee GS, Kim SM, Lim SM, Kim YS, Park SC. 2020. Current status of GM crop development and commercialization. Korean Journal of Breeding Science 52:40-48. [in Korean]  https://doi.org/10.9787/KJBS.2020.52.S.40
  7. Choi SS, Kim GD. 2022a. Production of carotenoids by bacteria; carotenoid productivity and availability. Journal of Life Science 32:411-419. [in Korean] 
  8. Choi WS, Ahn SJ, Yoon JH, Kim HH, Jang JW, Park JJ. 2015. Comparing of insect fauna between transgenic rice and common rice cultivar based on light tap and sweeping methods. Journal of Agriculture & Life Science 49:1-17. [in Korean] 
  9. Choi Y, Kim BS. 2022b. Domestic environmental release of genetically modified crops: Focusing on the government-NGOs joint environmental assessment. Civil Society and NGO 20:409-443. [in Korean] 
  10. Gullan PK, Cranston PS. 2005. The insect an outline of entomology. Blackwell Publishing Ltd., Oxford, UK. 
  11. Ha SH, Kim JB, Park JS, Lee SW, Cho KJ. 2007. A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: Deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper. Journal of Experimental Botany 58:3135-3144.  https://doi.org/10.1093/jxb/erm132
  12. Ha SH, Liang YS, Jung H, Ahn MJ, Suh SC, Kweon SJ, Kim DH, Kim YM, Kim JK. 2010. Application of two bicistronic synstems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm. Plant Biotech Journal 8:928-938.  https://doi.org/10.1111/j.1467-7652.2010.00543.x
  13. ISAAA (International Service for the Acquisition of Agri-biotech Applications). 2020. Global status of commercialized biotech/GM crops:2019, Brief No. 55-2019. International Service for the Acquisition of Agri-biotech Applications, New York, USA. 
  14. KBCH (Korea Biosafety Clearing House). 2020. Results of public awareness survey of LMO in 2020. KBCH, Daejeon, Korea. [in Korean] 
  15. Kim DY, Eom MS, Kim HJ, Ko EM, Pack IS, Park JH, Park KW, Nam KH, Oh SD, Kim JK, et al. 2020. Gene flow from transgenic soybean, developed to obtain recombinant proteins for use in the skin care industry, to non-transgenic soybean. Applied Biological Chemistry 63:65. 
  16. Kim KH, Kim DS, Park CG, Cho SW, Yoon YN, Lee KY. 2012b. Principles and application in insect pest management. Hyang Moon Sa, Seoul, Korea. [in Korean] 
  17. Kim KM, Ryu TH, Suh SJ. 2010. Studies on insect diversity related to genetically engineered vitamin A rice under large scale production. Korean Journal of Breeding Science 42:157-162. [in Korean] 
  18. Kim MJ, Kim JK, Kim HJ, Pak JH, Lee JH, Kim DH, Choi HK, Jung HW, Lee JD, Chung YS, et al. 2012a. Genetic modification of the soybean to enhance the β-carotene through seed-sepcific expression. PLoS One 7:e48287. 
  19. KOSTAT (Korean statistics). 2021. Results of a survey on the production of chinese cabbage, radish, soybean, apple and pear in 2021. Korean Statistics, Daejeon, Korea. [in Korean] 
  20. KRIBB (Korea Research Institute of Bioscience and Biotechnology). 2019. Biosafety white paper 2019. KRIBB, Daejeon, Korea. [in Korean] 
  21. Lee BK, Suh SC. 2011. A study on the trends and biosafety assessment of genetically modified crops. Environmental Law Review 33:3-25. [in Korean] 
  22. Lee CY, McCoon PE, LeBowitz JM. 1981. Vitamin A value of sweet corn. Journal of Agriculture and Food Chemistry 29:1294-1295.  https://doi.org/10.1021/jf00108a049
  23. Lee KJ, Shon SI, Lee JY, Yi BY, Oh SD, Kweon SJ, Suh SC, Ryu TH, Kim KH, Park JS. 2011b. Effects of transgenic soybean cultivation on soil microbial community in the rhizosphere. Korean Journal of Environmental Agriculture 30:466-472. [in Korean]  https://doi.org/10.5338/KJEA.2011.30.4.466
  24. Lee KJ, Yi BY, Kim KH, Kim JB, Suh SC, Woo HJ, Shin KS, Kweon SJ. 2011a. Development of efficient transformation protocol for soybean (Glycine max L.) and characterization of transgene expression after Agrobacterium-mediated gene transfer. Journal of the Korean Society for Applied Biological Chemistry 54:37-45. 
  25. Matthews PD, Luo R, Wurtzel ET. 2003. Maize phytoene desaturase and ζ-carotene desaturase catalyse a poly-Z desaturation pathway: Implications for genetic engineering of carotenoid content among cereal crops. Journal of Experimental Botany 54:2215-2230.  https://doi.org/10.1093/jxb/erg235
  26. Oh SD, Ha KH, Park SY, Lee SK, Yun DW, Lee KJ, Suh SJ. 2021. Impact of vitamin-A-enhanced transgenic soybeans on above-ground non-target arthropods in Korea. Korean Journal of Agricultural Science 48:875-890. [in Korean]  https://doi.org/10.7744/KJOAS.20210074
  27. Oh SD, Kim JS, Lee KJ, Ryu TH, Suh SJ. 2014. Studies on insect diversity related to drought-tolerant transgenic (Agb0103) rice under large scale GMO field. Korean Journal of Breeding Science 46:136-142. [in Korean]  https://doi.org/10.9787/KJBS.2014.46.2.136
  28. Oh SD, Lee KJ, Sohn SI, Kweon YJ, Kim JS, Lee JY, Park BS, Kweon SJ, Suh SC, Ryu TH, et al. 2012. Effect on insecticide susceptibility of Lissorhoptrus oryzophilus fed on Bacillus thuringiensis (Bt)-transgenic rice variety. Korean Journal of Environmental Agriculture 24:247-253. [in Korean] 
  29. Oh SD, Min SK, Kim JK, Park JH, Kim CG, Park SY. 2020a. Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna. Korean Journal of Agricultural Science 47:791-802. [in Korean]  https://doi.org/10.7744/KJOAS.20200066
  30. Oh SD, Park SY, Lee SK, Yun DW, Lee GS, Suh SJ. 2020b. Influence of vitamin A-enhanced transgenic soybean cultivation on the diversity of insects in LMO quarantine fields. Korean Journal of Breeding Science 52:310-321. [in Korean]  https://doi.org/10.9787/KJBS.2020.52.4.310
  31. Oh SD, Shin HC, Sohn SI, Lee KJ, Kim HJ, Ryu TH, Lee JY, Park BS, Kweon SJ, Suh SC, et al. 2011. Evaluation and assessment of biosafety for Bacillus thuringiensis (Bt)-transgenic rice: Responses of Daphnia magna fed on Bt-transgenic rice variety. Journal of Applied Biological Chemistry 54:296-302. [in Korean]  https://doi.org/10.3839/jabc.2011.048
  32. Oh SD, Suh SJ, Park SY, Lee KJ, Sohn SI, Yun DW, Chang AC. 2017. Effects of vitamin E enhanced transgenic soybean cultivation on insect diversity. Korean Journal of Breeding Science 49:129-140. [in Korean]  https://doi.org/10.9787/KJBS.2017.49.3.129
  33. Oh SW, Lee SM, Park SY, Lee SY, Lee WH, Cho HS, Yeo YS. 2016. Rice biotechnology and current development. Journal of the Korean Society of International Agriculture 28:24-36. [in Korean]  https://doi.org/10.12719/KSIA.2016.28.1.24
  34. Park TC, Choe HJ, Jeong HJ, Jang HJ, Kim JK, Park JJ. 2018. Comparison of insect fauna in transgenic and common rice paddy fields. Korean Journal of Environmental Biology 36:488-497. [in Korean]  https://doi.org/10.11626/KJEB.2018.36.4.488
  35. Tomkins A. 2001. Nutrition and maternal morbidity and mortality. British Journal of Nutrition 85:S93-S99. https://doi.org/10.1079/BJN2001358
  36. Vagadia BH, Vanga SK, Raghavan V. 2017. Inactivation methods of soybean trypsin inhibitor-a review. Trends in Food Science & Technology 64:115-125.  https://doi.org/10.1016/j.tifs.2017.02.003
  37. Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I. 2000. Engineering the provitamin A (β-carotene) biosynthetic pathway. Science 287:303-305.  https://doi.org/10.1126/science.287.5451.303