Mycobiology

  • 제50권1호
  • /
  • Pages.79-85
  • /
  • 2022
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Genetic Variation of Strawberry Fusarium Wilt Pathogen Population in Korea

  • Cho, Gyeongjun (Research Institute of Life Science, Gyeongsang National University) ;
  • Kwak, Youn-Sig (Research Institute of Life Science, Gyeongsang National University)
  • 투고 : 2021.09.29
  • 심사 : 2022.01.25
  • 발행 : 2022.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Strawberries are a popular economic crop, and one of the major plantations and exporting countries is Korea in the world. The Fusarium oxysporum species complex (FOSC) is a soil-borne pathogen with genetic diversity, resulting in wilt disease in various crops. In Korea, strawberries wilt disease was first reported in the 1980s due to the infection of FOSC, causing significant economic damage every year. The causal agent, F. oxysporum f. sp. fragariae, is a soil-borne pathogen with a characteristic of FOSC that is difficult to control chemically and mutates easily. This study obtained genetic polymorphism information that was based on AFLP, of F. oxysporum f. sp. fragariae 91 strains, which were isolated from strawberry cultivation sites in Gyeongsangnam-do and Chungcheongnam-do, and compared strains information, which was the isolated location, host variety, response to chemical fungicide, and antagonistic bacteria, and mycelium phenotype. As a result, AFLP phylogeny found that two groups were mainly present, and group B was present at a high frequency in Gyeongsangnam-do. Group B proved less sensitive to tebuconazole than group A through Student's t-test. In addition, the fractions pattern of AFLP was calculated by comparing the strain information using PCA and PERMANOVA, and the main criteria were separated localization and strawberry varieties (PERMANOVA; p< 0.05). And tebuconazole was different with weak confidence (PERMANOVA; p< 0.10). This study suggests that the F. oxysporum f. sp. fragariae should be continuously monitored and managed, including group B, which is less chemically effective.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MIST) [2020R1A2C2004177].

참고문헌

  1. Rana A, Sahgal M, Johri B. 2017. Fusarium oxysporum: genomics, diversity and plant-host interaction. In: Satyanarayana T, Deshmukh SK, Johri BN, editors. Developments in fungal biology and applied mycology. Singapore: Springer; p. 159-199.
  2. Edel-Hermann V, Lecomte C. Current status of Fusarium oxysporum formae speciales and races. Phytopathology. 2019;109(4):512-530. https://doi.org/10.1094/phyto-08-18-0320-rvw
  3. Ploetz RC. Fusarium wilt of banana. Phytopathology. 2015;105(12):1512-1521. https://doi.org/10.1094/PHYTO-04-15-0101-RVW
  4. Rubinstein J. 2015. Fragaria x ananassa: past, present and future production of the modern strawberry. University of Minnesota, University of Minnesota's Digital Conservancy, Minneapolis.
  5. Johnson H, Holland AH, Paulus AO, et al. Soil fumigation found essential for maximum strawberry yields in Southern California. Calif Agric. 1962;16(10):4-6.
  6. Cho CT, Moon BJ. Studies on the wilt of strawberry caused by Fusarium oxysporum f. sp. fragariae in korea. Korean J Appl Entomol. 1984;23(2):74-81.
  7. Koike ST, Gordon TR. Management of Fusarium wilt of strawberry. Crop Prot. 2015;73:67-72. https://doi.org/10.1016/j.cropro.2015.02.003
  8. Vos P, Hogers R, Bleeker M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995;23(21):4407-4414. https://doi.org/10.1093/nar/23.21.4407
  9. Kim JS, Kang NJ, Kwak YS, et al. Investigation of genetic diversity of Fusarium oxysporum f. sp. fragariae using PCR-RFLP. Plant Pathol J. 2017;33(2):140-147. https://doi.org/10.5423/PPJ.FT.01.2017.0011
  10. Chae DH, Kim DR, Kwak YS. Sensitivity variation of Fusarium oxysporum f. sp. fragariae, strawberry wilt pathogen against biocontrol agent. J Agirc Life Sci. 2021;55(3):33-42. https://doi.org/10.14397/jals.2021.55.3.33
  11. Suga H, Hirayama Y, Morishima M, et al. Development of PCR primers to identify Fusarium oxysporum f. sp. fragariae. Plant Dis. 2013;97(5):619-625. https://doi.org/10.1094/pdis-07-12-0663-re
  12. Kwon SK, Jeon CW, Nam MH, et al. Evaluation of fungicides sensitivities of strawberry wilt disease pathogen, Fusarium oxysporum f. sp. fragariae. Korean J Pestic Sci. 2020;24(1):34-42. https://doi.org/10.7585/kjps.2020.24.1.34
  13. Baayen RP, O'Donnell K, Bonants PJ, et al. Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease. Phytopathology. 2000;90(8):891-900. https://doi.org/10.1094/PHYTO.2000.90.8.891
  14. Covarrubias-Pazaran G, Diaz-Garcia L, Schlautman B, et al. Fragman: an R package for fragment analysis. BMC Genet. 2015;17(62):1-8.
  15. Paradis E, Schliep K. Ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R. Bioinformatics. 2019;35(3):526-528. https://doi.org/10.1093/bioinformatics/bty633
  16. Gross J, Ligges U, Ligges MU. 2015. nortest: Tests for normality. R package version 1.0-4. https://CRAN.R-project.org/package=nortest
  17. Oksanen J, Blanchet FG, Friendly M, et al. 2020. vegan: Community ecology package. R package version 2.5-7. https://CRAN.R-project.org/package=vegan.
  18. Arbizu PM. 2017. pairwiseAdonis: Pairwise multilevel comparison using Adonis. R package version 0.0.1.
  19. R Core Team. 2020. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing. https://www.R-project.org/.
  20. Ritz C, Baty F, Streibig JC, et al. Dose-response analysis using R. PloS One. 2015;10(12):e0146021. https://doi.org/10.1371/journal.pone.0146021
  21. Wickham H. ggplot2. WIREs Comp Stat. 2011;3(2):180-185. https://doi.org/10.1002/wics.147