한국균학회지 (The Korean Journal of Mycology)

  • 제50권3호
  • /
  • Pages.217-224
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  • 2022
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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국내 담수환경에서 분리된 국내 미기록 효모 6종 보고

Six Yeast Strains Isolated from Freshwaters for the First Record in Korea

  • 오유선 (국립낙동강생물자원관 미생물연구실, 균류연구팀) ;
  • 서민정 (국립낙동강생물자원관 미생물연구실, 균류연구팀) ;
  • 고재덕 (국립낙동강생물자원관 미생물연구실, 균류연구팀) ;
  • 문혜연 (국립낙동강생물자원관 미생물연구실, 균류연구팀)
  • Yoosun Oh (Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Min Jeong Seo (Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Jaeduk Goh (Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Hye Yeon Mun (Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources)
  • 투고 : 2022.07.25
  • 심사 : 2022.09.13
  • 발행 : 2022.09.30
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초록

본 연구는 광주광역시와 전라남도 여수시의 담수환경으로부터 야생효모를 분리하여 특성 분석을 하였다. 분리된 효모의 동정을 위하여 26S rDNA의 D1/D2부위염기서열을이용하였다. 결과적으로 Apiotrichum chiropterorum (NNIBRFG36995), A. domesticum (NNIBRFG32938), A. dulcitum (NNIBRFG33144), A. laibachii(NNIBRFG36991), S. quercus (NNIBRFG33183), Tausonia pullulans (NNIBRFG33181)등 6종의 야생효모들을 동정하였다. 이들 6종의 효모는 국내에 기록된 바가 없으며, 본 논문이 최초보고이다. 미기록 효모 모두 PD, YM 배지에서 잘 자랐으며, 15-30℃ 온도 범위에서 생육하였다. A. domesticum (NNIBRFG32938), A. laibachii (NNIBRFG36991), Tausonia pullulans (NNIBRFG33181) 균주들은 20% glucose를 함유한 YPD 배지에서 생육하는 내당성을 보였으며A. domesticum (NNIBRFG32938)과 A. laibachii (NNIBRFG36991)는 5% NaCl을 함유한 YPD 배지에서도 생육하여 내염성을 가졌다.

This study aimed to isolated wild yeasts from freshwater in Korea. The strains were identified by using the D1/D2 domains of the 26S rDNA regions. Consequently, six strains were named as Apiotrichum chiropterorum (NNIBRFG36995), A. domesticum (NNIBRFG32938), A. dulcitum (NNIBRFG33144), A. laibachii (NNIBRFG36991), Saprochaete quercus (NNIBRFG33183) and Tausonia pullulans (NNIBRFG33181). These yeasts have not previously been recorded in Korea, this paper is the first report. We were investigated to the morphological and cultural characteristics of these yeasts. All the strains grew well on Yeast extract peptone dextrose (YPD), Potato dextrose (PD), and Yeast mold (YM) media and in temperature range of 15-30℃. A. domesticum (NNIBRFG32938), A. laibachii (NNIBRFG36991) and T. pullulans (NNIBRFG33181) grew even in 20% glucose containing YPD medium, so they had glucose tolerance. A. domesticum (NNIBRFG32938) and A. laibachii (NNIBRFG36991) had salt resistance as growing even in 5% NaCl containing YPD medium.

키워드

과제정보

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR202201107).

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