Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Mass rearing system for Neodryinus typhlocybae(Hymenoptera: Dryinidae) as a biological control agent of Metcalfa pruinosa

미국선녀벌레 천적인 선녀벌레집게벌 대량사육 체계

  • Meeja Seo (Crop Protection Division, Dept. of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Jeong Hwan Kim (Crop Protection Division, Dept. of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Hyeon Jung Noh (Crop Protection Division, Dept. of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Bo Yoon Seo (Crop Protection Division, Dept. of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Jum Rae Cho (Crop Protection Division, Dept. of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Hong Hyun Park (Crop Protection Division, Dept. of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA)
  • 서미자 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 김정환 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 노현정 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 서보윤 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 조점래 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 박홍현 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과)
  • Received : 2022.10.31
  • Accepted : 2022.11.17
  • Published : 2022.12.31
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Abstract

The mass-rearing system for Neodryinus typhlocybae as a biological control agent of Metcalfa pruinosa was established. Depending on the density of host nymphs and plants, the average number of cocoons produced by the parasitoids was 5-8 and 70-150 cocoons per leaf and sapling of mulberry, respectively. There is a significant difference in cocoon length between females (6.10-6.46mm) and males (4.20-4.62mm). Sex determination of cocoons before emergence will be helpful for efficiently releasing this parasitoid in fields. The parasitic rate of N. typhlocybae at the semi-field condition was on average 13-17%. The release number of this parasitoid did not affect parasitism. Nevertheless, the population growth rate of M. pruinosa was reduced by increasing the release number of N. typhlocybae. The parasitoid offspring's sex and bivoltine were influenced by the host age. On young host nymphs, the bivoltine portion of parasitoid increased. When parasitized on 4th or 5th nymphs, the offspring's female ratio of N. typhlocybae increased. This result may be useful for potentially controlling mass rearing production of parasitoid.

미국선녀벌레 생물적 방제를 위한 기생성 천적인 선녀벌레집게벌 대량사육 체계를 구축하였다. 선녀벌레집게벌 고치 생산은 기주인 미국선녀벌레 약충밀도와 기주식물의 재식밀도에 따른 차이를 보였지만, 뽕나무 기주식물 잎당 평균 5~8개, 주당 평균 70~150개가 생산되었다. 선녀벌레집게벌 암컷 평균 고치 길이는 6.01~6.46 mm, 수컷은 4.20~4.62 mm로 암수 간 통계적으로 유의미한 차이를 보였으며, 우화 전 고치상태에서의 암수 구분으로 선녀벌레집게벌을 효과적으로 방사할 수 있을 것으로 보인다. 반야외조건에서 선녀벌레집게벌 기생률은 평균 13~17%로, 방사 마리수에 따른 기생률 차이는 확인되지 않았으나 방사 마리수가 많을수록 미국선녀벌레 개체군밀도증가율이 낮아지는 경향을 보였다. 선녀벌레집게벌의 성비나 이화성은 기주의 영기에 따라 달라졌다. 기주의 영기가 낮을수록 이화성 비율이 증가했고, 4~5령 약충에 기생시 암컷의 비율이 높게 나타나 대량사육 시 개체조절이 가능할 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 공동연구사업(침입해충 방제제의 방제효율 증대 기술 개발, 과제번호: PJ014845) 지원에 의해 수행되었으며 이에 감사드립니다.

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