Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immunostimulatory Effect of Heat-Killed Probiotics on RAW264.7 Macrophages

  • Noh, Hye-Ji (Probiotics Research Laboratory, Chong Kun Dang Bio Research Institute (CKDBIO)) ;
  • Park, Jung Min (R&D Center, Chong Kun Dang Healthcare (CKDHC)) ;
  • Kwon, Yoo Jin (Probiotics Research Laboratory, Chong Kun Dang Bio Research Institute (CKDBIO)) ;
  • Kim, Kyunghwan (Probiotics Research Laboratory, Chong Kun Dang Bio Research Institute (CKDBIO)) ;
  • Park, Sung Yurb (R&D Center, Chong Kun Dang Healthcare (CKDHC)) ;
  • Kim, Insu (R&D Center, Chong Kun Dang Healthcare (CKDHC)) ;
  • Lim, Jong Hyun (R&D Center, Chong Kun Dang Healthcare (CKDHC)) ;
  • Kim, Byoung Kook (Probiotics Research Laboratory, Chong Kun Dang Bio Research Institute (CKDBIO)) ;
  • Kim, Byung-Yong (R&D Center, Chong Kun Dang Healthcare (CKDHC))
  • Received : 2022.01.14
  • Accepted : 2022.03.18
  • Published : 2022.05.28
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Abstract

Probiotics modulate the gut microbiota, which in turn regulate immune responses to maintain balanced immune homeostasis in the host. However, it is unclear how probiotic bacteria regulate immune responses. In this study we investigated the immunomodulatory effects of heat-killed probiotics, including Lactiplantibacillus plantarum KC3 (LP3), Lactiplantibacillus plantarum CKDB008 (LP8), and Limosilactobacillus fermentum SRK414 (LF4), via phagocytosis, nitric oxide (NO), and pro-inflammatory cytokine production in macrophages. We thus found that heat-killed LP8 could promote the clearance of foreign pathogens by enhancing the phagocytosis of macrophages. Treatment with heat-killed LP8 induced the production of NO and pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β. In addition, heat-killed LP8 suppressed the production of NO and cytokines in LPS-induced RAW264.7 cells, suggesting that heat-killed LP8 exerts immunomodulatory effects depending on the host condition. In sum, these results indicate that heat-killed LP8 possesses the potential for immune modulation while providing a molecular basis for the development of functional probiotics prepared from inactivated bacterial cells.

Keywords

References

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