DOI QR코드

DOI QR Code

Protective Effect of Paulownia tomentosa Fruits in an Experimental Animal Model of Acute Lung Injury

  • Kim, Seong-Man (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Ok-Kyoung (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Min, Jae-Hong (Laboratory Animal Resources Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex) ;
  • Park, Jin-Mi (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Doo-Young (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Sei-Ryang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Seung Jin (College of Pharmacy, Chungnam National University) ;
  • Ahn, Kyung-Seop (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Jae-Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 투고 : 2021.12.29
  • 심사 : 2022.05.10
  • 발행 : 2022.06.28

초록

The fruits of Paulownia tomentosa (Thunb.) (PT) Steud. have been reported to exert a variety of biological activities. A previous study confirmed that compounds isolated from PT fruits (PTF) exerted anti-inflammatory effects on TNF-α-stimulated airway epithelial cells. However, there is no report on the protective effects of PTF on acute lung injury (ALI). Here, we examined the ameliorative effects of PTF in an experimental animal model of lipopolysaccharide (LPS)-induced ALI. In ALI mice, increased levels of inflammatory cell influx were confirmed in the lungs of mice, and an increase of microphage numbers, TNF-α, IL-6 and MCP-1 production and protein content were detected in mouse bronchoalveolar lavage fluid. However, these increases were significantly reversed with PTF pretreatment. In addition, PTF inhibited the increased expression of iNOS and COX-2 in the lungs of ALI mice. Furthermore, the upregulation of MAPK and NF-κB activation was decreased in the lungs of ALI mice by PTF. In the in vitro experiment, PTF pretreatment exerted an anti-inflammatory effect by inhibiting the secretion of nitric oxide, TNF-α and IL-6 in LPS-stimulated RAW264.7 macrophages. Collectively, these results indicated that PTF has ameliorative effects on airway inflammation in an experimental animal model of ALI.

키워드

과제정보

This study was supported by grants from the Korea Research Institute of Bioscience and Biotechnology Research Initiative Program (KRIBB) of the Republic of Korea (grant. nos. KGM5522211 and KGS1232221).

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