Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Improved Ectoine Production from Methane by Optimization of the Bio-milking Process in Engineered Methylomicrobium alcaliphilum 20Z

  • Lee, Yun Seo (C1 Gas Refinery R&D Center, Sogang University) ;
  • Chai, Hanyu (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Cho, Sukhyeong (C1 Gas Refinery R&D Center, Sogang University) ;
  • Na, Jeong Geol (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Jinwon (C1 Gas Refinery R&D Center, Sogang University)
  • Received : 2021.12.14
  • Accepted : 2022.02.09
  • Published : 2022.08.01
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Abstract

Methane is one of the major greenhouse gases, recently, the biotechnological conversion from methane to high-value added chemicals have emerged as an effort to reduce methane gas emission. In this study, we optimized ectoine bio-milking conditions in which cells were repeatedly used to improve intracellular and extracellular ectoine yield from methane by using Methylomicrobium alcaliphilum 20ZDP2. First, the cultivation and intracellular ectoine accumulation conditions were optimized with respect to the growth phase and medium salinity to achieve the highest yield of synthesis. Second, ectoine excretion was optimized by determining the ectoine secretion time (15 min) in appropriate medium salinity under hypoosmotic conditions (1% NaCl). Finally, bio-milking of ectoine was successfully repeated more than 10 times using M. alcaliphilum 20ZDP2, and the ectoine yield was improved up to 129.29 mg/ DCW g.

Keywords

Acknowledgement

This research was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT (2015M3D3A1A01064929).

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