Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Dermal and Eye Irritation Studies on Bactonematicide, Photorhabdus temperata Isolated from Heterorhabditis megidis (Nematoda: Heterorhabditidae) in Rabbit

Heterorhabditis megidis (Nematoda: Heterorhabditidae)에서 분리한 Bactonematicide, Photorhabdus temperata의 토끼 피부 및 눈자극 시험

  • Park, Sun-Han (School of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University) ;
  • Chung, Nam-Jun (Nambo Co., Ltd.) ;
  • Choo, Young-Moo (Jinju Bioindustry Foundation) ;
  • Kim, Young-Joon (Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Jin-Ho (School of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University)
  • Received : 2022.06.08
  • Accepted : 2022.07.19
  • Published : 2022.08.31
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Abstract

The acute dermal and eye irritation tests of Photorhabdus temperata, a symbiotic bacterium of Heterorhabditis megidis Gwangju strain, were carried out in New Zealand white rabbit (Oryctolagus cuniculus), following the guidelines of OECD and Rural Development Administration (RDA) of Korea. In both tests, neither dermal nor eye responses were found from all the P. temperata treated rabbits and the results were classified as non-irritating. That is, erythema, eschar, edema, and any other dermal critical signs were not observed from all the experimental rabbits in the dermal irritation test. In the acute eye irritation test, no clinical signs of cornea, iritis, conjunctiva (redness, edema, lacrima, and chemosis) were observed from all the experimental rabbits. Individual ocular irritation, mean ocular irritation, and acute ocular irritation were calculated as 0.0. The results of dermal and eye irritation studies on P. temperata indicated that this bacterium could be a safe and effective alternative bionematicide against the most serious plant-parasitic root-knot nematodes in the genus Meloidogyne.

Keywords

Acknowledgement

This research was financially supported by the Ministry of Agriculture Food and Rural Affairs (MAFRA), Korea, under the "Development of Industrialization Technology for Crop Viruses and Disease Pests" (Ref. No. 120081-05) supervised by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry.

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