Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Lonicera japonica inhibited the oxidative Stress induced by the heavy metal

중금속 유도 산화적 스트레스에 대한 금은화의 세포 보호 효과

  • Yeom, Seung-Hee (School of Korean Medicine, Dongguk University) ;
  • Bak, Seon Been (School of Korean Medicine, Dongguk University) ;
  • Park, Sun-Dong (School of Korean Medicine, Dongguk University) ;
  • Park, Kwang-Il (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Young Woo (School of Korean Medicine, Dongguk University)
  • 염승희 (동국대학교 한의학과 방제학교실) ;
  • 박선빈 (동국대학교 한의학과 방제학교실) ;
  • 박선동 (동국대학교 한의학과 방제학교실) ;
  • 박광일 (경상대학교 수의과대학 수의생리학) ;
  • 김영우 (동국대학교 한의학과 방제학교실)
  • Received : 2022.08.10
  • Accepted : 2022.08.26
  • Published : 2022.08.31
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Abstract

Objectives : Lonicera japonica is known for anti-inflammation and antibiotic effect in Korean medicine. This study aimed for investigating the cytoprotective effect of Lonicera japonica extract (LJE) for HepG2 cells against arachidonic acid (AA)+iron-induced oxidative stress. Methods : The effect of LJE on cell viability was assessed by MTT assay. ROS assay was selected to assess antioxidant effect of LJE. To assess LJE's effect on mitochondrial function, flow cytometric analysis was operated. And immunoblot analysis was used to establish the underlying mechanism of LJE. Results : LJE protected HepG2 cells against AA+iron-induced oxidative stress by phosphorylation of liver kinase B1 and blocked the decline of procaspase 3. Also, LJE preserved the mitochondrial membrane permeability induced by AA+iron. Conclusion : LJE protected the hepatocyte from AA+iron-induced oxidative stress by activation of LKB1 by the preservation of mitochondrial functions.

Keywords

Acknowledgement

This work was supported by National Research Foundation (NRF) grant funded by Korea government (No. 2022R1I1A3053818), and by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (number: HF20C0212).

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